Can ultrasound counteract bone loss? Effect of low-intensity ultrasound stimulation on a model of osteoclastic precursor

A Feasibility of Low Intensity Ultrasound Stimulation for Treatment or Prevention of Osteoporosis and Its-Related Fracture

“…LIUS could improve the defective or damaged bone healing; enhancement of the mechanical properties on the healing callus, bone bridging, nonunion fractures healing and distraction osteogenesis and reduction of healing time (Eberson, 2003;Pilla et al, 1990). Furthermore, in vitro cellular studies supported these in-vivo results (Kokubu et al, 1999;Li et al, 2003;Monici et al, 2007;Naruse et al, 2000;Unsworth et al, 2007;Yang et al, 2005) . LIUS could regulate bone cells; enhancing osteoblast formation and function and suppressing osteoclast formation and function.…”

“…Based on these rationales, external biophysical stimuli have been suggested as alternatives to pharmacological therapies. Ultrasound stimulation is one such promising stimulus (Monici et al, 2007;Perry et al, 2009;Rubin et al, 2001b). Ultrasound is a high-frequency non-audible acoustic pressure wave with mechanical energy and can be transmitted at osteoporotic sites through biological tissues.…”

Effect of low-intensity pulsed ultrasound on the activity of osteoclasts: An in vitro study

“…This hypothesis requires further validation. Monici et al (2007) investigated the behavior of an osteoclast precursor cells (FLG 29.1 human cell line) after different regimens of LIPUS application for 2 days. They reported that ultrasound stimulation decreased the expression of cytoskeletal components and markers of cell growth and differentiation, and induced cell damage dose-dependently.…”

“…To the best of our knowledge, there is only a couple of in vitro experiments that investigated the effect of LIPUS on OC-only cultures (Monici et al, 2007;Chen, Wu, Wang, & Li, 2012). Therefore, the objective of this in vitro experiment was to evaluate the effect of LIPUS on the resorption activity of OC-like cells.…”

Application of Ultrasound Stimulation in Bone Tissue Engineering

“…Less than 120 mW/cm 2 of intensity and 1∼3 MHz of frequency were considered good condition for regeneration of bone tissue (9)(10)(11). Increased osteoblast cells and higher mineralized nodule formation explain the enhancement of proliferation by LIPUS (12,22,23). In addition, LIPUS affects on differentiation of osteoblast cells, which is shown by increased ALPase, and transcriptional factors, Runx2 (3,22,(24)(25)(26)(27)(28).…”