Çanakkale ili börülce üretim alanlarında Hıyar mozaik virüsü (Cucumber mosaic virus; CMV)’nün tespiti ve kılıf protein genine göre moleküler karakterizasyonu

Abstract

In 2015-2016, surveys were carried out in areas of Çanakkale province where cowpea and bean were cultivated. Twenty-two samples (14 bean and 8 cowpea) showing virus and virus-like symptoms were collected from these areas. The collected samples were tested by DAS-ELISA to determine the presence of Cucumber mosaic virus (CMV) infection and 6 samples out of 22 were found to be infected with CMV. While the determined all CMV infection was found in cowpea samples, there was no infection found in bean samples. Coat protein (CP) gene of samples infected with CMV was amplified with RT-PCR and corresponding bands of 638 bp were obtained from all of them. RT-PCR products of CWP17 isolate chosen from infected samples randomly was purified and sequenced. Sequence data of CWP17 isolate was deposited (accession number: KY474380) in GenBank. Results of multiple sequence alignment analysis, CWP17 isolate was found to be identical 80-99% and 90-100% with world CMV isolates in nucleotide and amino acid level, respectively. Also, CWP17 isolates was found in Subgroup IB accordingly phylogenetic tree created. With carrying out this study, CMV infection was the first time detected in commercial cowpea production areas in Turkey. In addition, the first molecular characterization work was carried out in legume production areas of Turkey.

The molecular characterization of the coat protein sequence and differentiation of CMV- subgroup I on tobacco from native flora in Turkey

“…Based on previous studies carried out in Turkey, CMV SI-A has been found in pepper, tomato and watermelon (Caglar, 2006), CMV SI and SII in pepper, tomato, cucumber, watermelon and melon (Sarı, 2015), CMV SI-B in cowpea (Karanfil and Korkmaz, 2017) and, CMV SI-A in spinach (Kurtoglu and Korkmaz, 2018). Even though CMV has been detected on a wide range of hosts in Turkey, subgroup categories and their classification of most of them were not currently completed at the nucleotide levels.…”

“…The presence of CMV in tobacco has been reported by several researchers as both natural hosts (Chatzivassiliou et al, 2004;Dai et al, 2012;Zhang et al, 2013) and experimental host (Tian et al, 2009;Chikh Ali et al, 2012). It has a nationwide host range with the exception of tobacco, which includes: Myrtle leaf milkwort, globe artichoke, parsley, mint, broccoli, squash, olives, cabbage, peppers, lettuce, spinach, cowpea, gladiolus, tomatoes, zucchini, cucumber, ornamental plants (daffodils, hyacinths, lilies), bean and spinach (Gümüs et al, 2004;Beler and Acıkgöz, 2005;Sevik and Akcura, 2011;Culal Kılıc and Yardımcı, 2012;Erkan et al, 2013;Ergün et al, 2013;Culal Kılıc et al, 2015;Uzunoğulları and Gümüs, 2015;Sertkaya, 2015;Karanfil et al, 2016;Gökdag et al, 2016;Güngör et al, 2017;Karanfil and Korkmaz, 2017;Koc and Fidan, 2017;Sevik, 2012Sevik, , 2017. Since its transmission to tobacco is not seed-borne (De Bokx and Huttinga, 1981), its presence and prevalence can stem from other virus sources like weeds, the suitable climate environment for active aphid populations throughout tobacco production season and acquired from other cultivated plants grown from CMV-infected seeds (Kaplan et al, 1997;Tsitsipis et al, 2001).…”

Türkiye’nin Bingöl ilinde Virüs Belirtisi Gösteren Kavun Bitkilerinde Hıyar mozaik virüsü ve Karpuz mozaik virüsünün Varlığı ve Filogenetik Yakınlıkları

“…As myrtifolia, bean and spinach, pepper, tomato, cucumber, olive, and others (Beler and Açıkgöz, 2005;Çulal Kılıç and Yardımcı, 2012;Ergün et al, 2013;Erkan et al, 2013;Gökdağ et al, 2016;Güngör et al, 2017;Gümüş et al, 2004;Karanfil and Korkmaz, 2017;Karanfil et al, 2016;Sevik and Akcura, 2011;Sevik, 2012;Sertkaya, 2015, Uzunoğulları andGümüş, 2015). Figure 3.…”

Samsun İli Kanola Üretim Alanlarda Enfeksiyon Oluşturan Virüslerin Belirlenmesi

“…TuMV, afit türleri tarafından kolayca taşınabilmektedir [13]. Ülkemizin birçok bölgesinde farklı illerde lahanagil bitkilerinde TuMV tespit edilmiş ve populasyon yapıları aydınlatılmıştır [14][15][16].…”

“…Ülkemizde kanola bitkilerinde virüs enfeksiyonu Trakya Bölgesi'nde yürütülen çalışmalar ile tespit edilmiştir. Bu çalışmalar ülkemizde kanola virüsleri ile ilgili yapılan ilk araştırmalardır [16]. Ancak Karadeniz Bölgesi'nde Samsun ilinde özellikle birçok lahanagil grubu bitki türlerinin yoğun olduğu ve kanola üretiminin yapıldığı önemli tarımsal potansiyele sahip bir bölgede, kanola hastalıklarına yönelik herhangi bir çalışma bulunmamaktadır.…”