Cancer associated ﬁbroblasts are distinguishable from peri‑tumor ﬁbroblasts by biological characteristics in TSCC

Ba, Pengfei; Zhang, Xiaojuan; Yu, Miao; Li, Linxia; Duan, Xiaoyu; Wang, Mingying; Lv, Shuyan; Fu, Guo; Yang, Pishan; Yang, Chengzhe; Qi-zhong, Sun

doi:10.3892/ol.2019.10556

Cited by 6 publications

(6 citation statements)

References 32 publications

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“…S3D). Moreover, we found that TAFs are morphologically distinct from CAFs, consistent with a study of TAFs, which again referred them as “peri-tumor fibroblasts,” isolated from tongue squamous cell carcinoma and showed different cell organization in coculture compared to CAFs (45). Ba et al observed that CAFs grow in a more disorganized manner compared to peri-tumor fibroblasts.…”

Section: Discussionsupporting

confidence: 90%

Multi-Omics Analysis of Fibroblasts from the Invasive Tumor Edge Reveals that Tumor-Stroma Crosstalk Induces O-glycosylation of the CDK4-pRB Axis

Bouchard

Fjg²,

et al. 2021

Preprint

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The invasive leading edge represents a potential gateway for tumor invasion. We hypothesize that crosstalk between tumor and stromal cells within the tumor microenvironment (TME) results in the activation of key biological pathways depending on their location in the tumor (edge vs core). Here, we highlight phenotypic differences between Tumor-Adjacent-Fibroblasts (TAFs) from the invasive edge and Cancer-Associated Fibroblasts (CAFs) from the tumor core, established from human lung adenocarcinomas. We use an innovative multi-omics approach that includes genomics, proteomics and, O-glycoproteomics to characterize crosstalk between TAFs and cancer cells. Our analysis shows that O-glycosylation, an essential post-translational modification resulting from sugar metabolism, alters key biological pathways including the CDK4-pRB axis in the stroma, and indirectly modulates pro-invasive features of cancer cells. In summary, aside from improving the efficacy of CDK4 inhibitors anti-cancer agents, the O-glycoproteome poses a new consideration for important biological processes involved in tumor-stroma crosstalk.

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Section: Discussionsupporting

confidence: 90%

Multi-Omics Analysis of Fibroblasts from the Invasive Tumor Edge Reveals that Tumor-Stroma Crosstalk Induces O-glycosylation of the CDK4-pRB Axis

Bouchard

Fjg²,

et al. 2021

Preprint

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“…Our previous work has indicated that CAFs are distinguishable from PTFs in TSCC by specific biological characteristics, and PTFs could be used as control cells for investigating CAFs (Ba et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…All procedures were approved by the Medical Ethics Committee of Qilu Hospital (Shandong University; 2016015), and all six participants were informed of the procedures and signed the consent form. The separation, purification, and identification of CAFs were performed as described in our previous publication (Ba et al, 2019).…”

Section: Cell and Cell Culturementioning

confidence: 99%

“…Cancer-associated fibroblasts/PTFs were seeded into 6-well plates (approximately 1 × 10 5 cells/well) overnight. Then, the cells were exposed to 0-10 μM curcumin for 24 hr, after which the whole-cell protein was extracted and quantitated using the protocol described previously (Ba et al, 2019). Proteins (20 μg/ lane) from different samples were separated through 10% SDS-PAGE (Boster) and transferred to 0.2-μm polyvinylidene fluoride (PVDF) membranes (Millipore).…”

Section: Western Blottingmentioning

confidence: 99%

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Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer‐associated fibroblasts of TSCC

et al. 2020

Oral Diseases

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Cancer‐associated fibroblasts (CAFs) are “activated” fibroblasts in the tumor microenvironment (TME) and play a vital role in all steps of cancer development. Increasing evidence focusing on the function of CAFs suggests that CAFs are candidate therapeutic targets and that drugs targeting the modification of CAFs would suppress tumor progression and be beneficial to tumor treatment and prevention. In the present study, we found that curcumin reversed the phenotype of CAFs to that of peri‐tumor fibroblast (PTF)‐like cells by downregulating the expression of α‐SMA (a special marker for CAFs) and inhibiting the secretion of pro‐carcinogenic cytokines, including transforming growth factor‐β1 (TGF‐β1), matrix metalloproteinases 2 (MMP2), and stromal cell‐derived factor‐1 (SDF‐1). We further demonstrated that the conditioned medium (CM) derived from CAFs promoted the proliferation of Cal27, and this effect was confirmed by the xenograft model. More importantly, we found that curcumin blocked the CAF‐mediated enhancement of Cal27 proliferation in vitro and in vivo. In conclusion, our data suggest that curcumin reverses cell phenotype from CAF to PTF‐like cells and suppresses the CAF‐mediated proliferation and tumorigenicity of Cal27 by inhibiting TSCC CAFs.

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“…Esse mecanismo foi demonstrado, pela primeira vez, em nosso estudo em pacientes com CECCO. Da mesma forma, Ba et al163 avaliaram a diferença no comportamento biológico de CAFs e fibroblastos peritumorais, e identificaram que a expressão de MMP-2, analisada por PCRqt e ELISA, foi superior no grupo dos CAFs em relação aos fibroblastos peritumorais.…”

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Expressão de micro-RNAs associados a invasão tumoral e prognóstico em carcinoma de células escamosas de cavidade oral

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Cancer associated ﬁbroblasts are distinguishable from peri‑tumor ﬁbroblasts by biological characteristics in TSCC

Cited by 6 publications

References 32 publications

Multi-Omics Analysis of Fibroblasts from the Invasive Tumor Edge Reveals that Tumor-Stroma Crosstalk Induces O-glycosylation of the CDK4-pRB Axis

Multi-Omics Analysis of Fibroblasts from the Invasive Tumor Edge Reveals that Tumor-Stroma Crosstalk Induces O-glycosylation of the CDK4-pRB Axis

Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer‐associated fibroblasts of TSCC

Expressão de micro-RNAs associados a invasão tumoral e prognóstico em carcinoma de células escamosas de cavidade oral

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