Cancer Cells’ Metabolism Dynamics in Renal Cell Carcinoma Patients’ Outcome: Influence of GLUT-1-Related hsa-miR-144 and hsa-miR-186

Morais, Mariana; Dias, Francisca; Nogueira, Inês; Leão, Anabela; Gonçalves, Nuno; Araujo, L; Granja, Sara; Baltazar, Fátima; Teixeira, Ana Luísa; Medeiros, Rui

doi:10.3390/cancers13071733

Cited by 16 publications

(10 citation statements)

References 40 publications

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“…Currently, the functions of hsa-miR-144-5p and hsa-miR-506-3p have been reported mainly in the cancer field. High plasma levels of hsa-miR-144-5p were shown to be associated with renal cell carcinoma [ 30 ], non-small-cell lung cancer [ 31 ] or glioblastoma [ 32 ]. Recent research by Zhang et al showed that miR-144-5p can reduce bone repair and regeneration in type 2 diabetes by suppressing the expression of Smad1 [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Bone-Metabolism-Related Serum microRNAs to Diagnose Osteoporosis in Middle-Aged and Elderly Women

Zhao

Wen

et al. 2022

Diagnostics

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Objective: Postmenopausal osteoporosis (PMOP), a chronic systemic metabolic disease prevalent in middle-aged and elderly women, heavily relies on bone mineral density (BMD) measurement as the diagnostic indicator. In this study, we investigated serum microRNAs (miRNAs) as a possible screening tool for PMOP. Methods: This investigation recruited 83 eligible participants from 795 community-dwelling postmenopausal women between June 2020 and August 2021. The miRNA expression profiles in the serum of PMOP patients were evaluated via miRNA microarray (six PMOP patients and four postmenopausal women without osteoporosis (n-PMOP) as controls). Subsequently, results were verified in independent sample sets (47 PMOP patients and 26 n-PMOP controls) using quantitative real-time PCR. In addition, the target genes and main functions of the differentially expressed miRNAs were explored by bioinformatics analysis. Results: Four highly expressed miRNAs in the serum of patients (hsa-miR-144-5p, hsa-miR-506-3p, hsa-miR-8068, and hsa-miR-6851-3p) showed acceptable disease-independent discrimination performance (area under the curve range: 0.747–0.902) in the training set and verification set, outperforming traditional bone turnover markers. Among four key miRNAs, hsa-miR-144-5p is the only one that can simultaneously predict changes in BMD in lumbar spine 1–4, total hip, and femoral neck (β = −0.265, p = 0.022; β = −0.301, p = 0.005; and β = −0.324, p = 0.003, respectively). Bioinformatics analysis suggested that the differentially expressed miRNAs were targeted mainly to YY1, VIM, and YWHAE genes, which are extensively involved in bone metabolism processes. Conclusions: Bone-metabolism-related serum miRNAs, such as hsa-miR-144-5p, hsa-miR-506-3p, hsa-miR-8068, and hsa-miR-6851-3p, can be used as novel biomarkers for PMOP diagnosis independent of radiological findings and traditional bone turnover markers. Further study of these miRNAs and their target genes may provide new insights into the epigenetic regulatory mechanisms of the onset and progression of the disease.

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Section: Discussionmentioning

confidence: 99%

Bone-Metabolism-Related Serum microRNAs to Diagnose Osteoporosis in Middle-Aged and Elderly Women

Zhao

Wen

et al. 2022

Diagnostics

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“…Higher levels of miR-186-5p in ccRCC individuals dysregulates glucose transporter 1 (GLUT-1), which is responsible for basal glucose uptake to sustain cellular respiration. A disrupted expression of GLUT-1 results in disturbed cellular metabolism, a significant hallmark of cancer [24]. In colorectal neoplasms it was found that miR-627-5p serum levels were increased that leads to NUP160 suppression resulting in cell proliferation inhibition and stimulation of autophagy and apoptosis [25].…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel miRNAs Involved in Cancer Progression and Metastasis in Clear Cell Renal Cell Carcinoma

Hamoode¹,

Rafaa²,

Abduljaleel³

et al. 2022

Math.Biol.Bioinf.

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MicroRNAs are non-coding molecules 21–23 nucleotides long that are involved in post-transcriptional regulation of gene expression by mRNA silencing. There is evidence that miRNA activity is associated with various types of human cancer, such as breast cancer, colorectal cancer, ovarian cancer and hepatocellular carcinoma, as well as with clear cell renal cell carcinoma. In 2020, the incidence of kidney cancer worldwide was 319,016. Clear cell renal cell carcinoma is associated with loss or mutation of the VHL gene. The aim of this study was to identify miRNAs that are expressed differently in samples of normal kidney tissue and tissue affected by clear cell renal cell carcinoma. For this study, a public miRNA dataset from ArrayExpress was obtained and post-processed. Mapping, identification of known and new microRNAs, and quantification were performed using the miRDeep2 computer program from the DESeq R/Bioconductor package. We performed target identification and functional load analysis using GeneCodis4. A total of 2656 miRNAs were found to be differentially expressed, of which 229 miRNAs were overexpressed and 302 were underexpressed. In this study, we identified five miRNAs that were already known from the literature to be significantly associated with clear cell renal cell carcinoma. In addition, we found a set of five microRNAs that had not previously associated with clear cell renal cell carcinoma.

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“…The previous study for directly investigated the expression level of miR‐144‐5p in uremia was extremely limited. In other renal‐related diseases, such as renal cell carcinoma, it has been validated that miR‐144‐5p displays a low level in renal cell carcinoma patients, and the deficiency of miR‐144‐5p is associated with the worst overall survival [17]. It has been found that rat models of renal ischemia–reperfusion injury also exhibit decreased miR‐144‐5p expression [19].…”

Section: Discussionmentioning

confidence: 99%

“…It has been elucidated that miR-144-5p is silenced in renal cell carcinoma and human papillary thyroid carcinoma [16,17]. We examined miR-144-5p levels in the normal controls and the uremic patients.…”

Section: Mir-144-5p Is Downregulated In Uremic Patientsmentioning

confidence: 99%

Clinical expression of microRNA‐144‐5p and its regulatory effect on renal function in uremia

Zang

Li²,

Zhou

2022

Ther Apher Dial

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Objective The study commits to probing the clinical expression of microRNA‐144‐5p (miR‐144‐5p) and its modulatory effect on the renal function of uremia. Methods Levels of blood urea nitrogen (BUN), β2‐microglobulin (β2‐MG), serum creatinine (Scr), blood calcium (Ca), phosphorus (P), and intact parathyroid hormone (iPTH) and miR‐144‐5p expression in serum of uremia patients were detected. The correlation among miR‐144‐5p expression with BUN, β2‐MG, Scr, Ca, P, and iPTH levels in uremic patients was analyzed. The rats were injected with miR‐144‐5p agomir to detect the change of BUN, Scr, β2‐MG, Scr, Ca, P, and iPTH levels in uremic rats. Results miR‐144‐5p expression in uremic patients was negatively correlated with BUN, Scr, β2‐MG, P, and iPTH levels, and positively correlated with free Ca concentration in blood. miR‐144‐5p elevation reduced BUN, Scr, β2‐MG, P, and iPTH levels, and increased free Ca concentration in blood in uremic rats. Conclusion miR‐144‐5p is lowly expressed, and miR‐144‐5p has a regulatory effect on renal function in uremia.

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Cancer Cells’ Metabolism Dynamics in Renal Cell Carcinoma Patients’ Outcome: Influence of GLUT-1-Related hsa-miR-144 and hsa-miR-186

Cited by 16 publications

References 40 publications

Bone-Metabolism-Related Serum microRNAs to Diagnose Osteoporosis in Middle-Aged and Elderly Women

Bone-Metabolism-Related Serum microRNAs to Diagnose Osteoporosis in Middle-Aged and Elderly Women

Identification of Novel miRNAs Involved in Cancer Progression and Metastasis in Clear Cell Renal Cell Carcinoma

Clinical expression of microRNA‐144‐5p and its regulatory effect on renal function in uremia

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