Cancer characterization and diagnosis with SERS-encoded particles

Guerrini, Luca; Pazos-Pérez, Nicolás; Garcia‐Rico, Eduardo; Alvarez-Puebla, Ramón A.

doi:10.1186/s12645-017-0031-3

Cited by 61 publications

(50 citation statements)

References 111 publications

(162 reference statements)

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“…One can see from Figures 2(a)-2(f) that the main peaks observed for the tissue samples correspond to DNA/RNA, amino acids, lipids, and proteins. Our analysis confirms preliminary observation by González-Solís et al [33], Guerrini et al [34], Karabeber et al [35], and our previous experiments using spontaneous Raman spectroscopy [7][8][9][10][11][12][13]. More detailed analysis of band intensity at ∼1396 cm −1 observed in all SERS spectra of the three proposed methods shows that the mean ± SD are 527.1 ± 94.9, 906.5 ± 281.9, and 15.2 ± 3.3 for NP nos.…”

Section: Resultssupporting

confidence: 93%

Surface-Enhanced Raman Spectroscopy Analysis of Human Breast Cancer via Silver Nanoparticles: An Examination of Fabrication Methods

Brożek-Płuska

Kopeć

Surmacki

2018

Journal of Spectroscopy

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Breast cancer in a traditional way is diagnosed using mammography, computer tomography, ultrasounds, biopsy, and finally, histopathological analysis. Histopathological analysis is a gold standard in breast cancer diagnostics; however, it is time consuming and prone to the human interpretations. That is why new methods based on optical properties of analyzed human tissue samples are needed to be introduced to the clinical practice objective, costless and fast diagnostic protocols. Nowadays, Raman spectroscopy-based methods are gaining more and more importance. Raman spectroscopy and imaging allow to characterize human tissue samples using an electromagnetic radiation from a safe range, and simultaneously, a minimal sample preparation is required. During measurements, a natural differentiation in tissues components’ scattering cross sections is used to build 2D and 3D maps of the chemical component distribution. The paper presents the application of SERS (surface-enhanced Raman spectroscopy) measurements for analysis of human breast cancer (adenocarcinoma). The advantages of SERS application in cancer diagnostics are also discussed. Moreover, the detailed chemical composition of human breast cancer tissue based on Raman bands of DNA/RNA, amino acids, lipids, and proteins which are significantly enhanced is presented. Three different methods of NP preparation are presented, and the effectiveness of Raman signal enhancement of Ag nanoparticles synthetized by these methods is compared. The enhancement effect of NPs synthetized by reduction of silver nitrate with sodium borohydride (method no. 1) and silver nitrate-hydroxylamine hydrochloride reduction (method no. 2) was stronger when compared with the polyol method (method no. 3). Presented SERS results confirmed that the clearly resolved and high-intensity Raman spectra of cancer human breast tissue can be recorded using integration times of the order of fractional seconds and one milliwatt of the excitation laser power.

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Section: Resultssupporting

confidence: 93%

Surface-Enhanced Raman Spectroscopy Analysis of Human Breast Cancer via Silver Nanoparticles: An Examination of Fabrication Methods

Brożek-Płuska

Kopeć

Surmacki

2018

Journal of Spectroscopy

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“…Although the general use of SERS-active nanomaterials has been extensively reviewed in recent times, a specific focus on the latest usage of SERS-active nanomaterials for decoding tumor-host immune interactions is yet to be summarized [30,33,[39][40][41]. In a bid to provide a unique outline on the emerging use of SERS in the exciting field of cancer immunotherapy, we herein discuss the recent progress in SERS nanomaterials that have exhibited application potential for patient-tailored cancer immunotherapy selection and monitoring, including multiplexed in vitro and in vivo immune-sensing, -imaging, and -drug screening/delivery.…”

Section: Sers Immunomarker Sensing On Cells and In Circulationmentioning

confidence: 99%

“…However, both techniques are limited by image interpretation (i.e., pathology) expertise and variability or autofluorescence and photobleaching issues. In recent years, various new SERS-active nanomaterials have been developed to enhance performance in multiplexed SERS immunomarker imaging [40,[56][57][58][59][60].…”

Section: Sers Single Cell-immunoimagingmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies

2020

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Cancer immunotherapy encompasses a variety of approaches which target or use a patient’s immune system components to eliminate cancer. Notably, the current use of immune checkpoint inhibitors to target immune checkpoint receptors such as CTLA-4 or PD-1 has led to remarkable treatment responses in a variety of cancers. To predict cancer patients’ immunotherapy responses effectively and efficiently, multiplexed immunoassays have been shown to be advantageous in sensing multiple immunomarkers of the tumor microenvironment simultaneously for patient stratification. Surface-enhanced Raman spectroscopy (SERS) is well-regarded for its capabilities in multiplexed bioassays and has been increasingly demonstrated in cancer immunotherapy applications in recent years. This review focuses on SERS-active nanomaterials in the modern literature which have shown promise for enabling cancer patient-tailored immunotherapies, including multiplexed in vitro and in vivo immunomarker sensing and imaging, as well as immunotherapy drug screening and delivery.

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“…The SERS spectrum provides a complete structural characterization of target molecule functionalizing AuNPs being SERS a vibrational fingerprint, which characterizes the molecular chemical bonds and symmetry without the need for staining or expressing fluorogenic proteins [ 78 , 79 ]. Owing to its ultrahigh sensitivity, non-destructive character, specificity and due to the possibility to perform Raman measurements under infrared excitation SERS is readily applicable in vivo with negligible background signal [ 80 ]. By using the SERS technique in combination with near-infrared absorbing molecules in resonance with the excitation laser line, one can develop promising ultrasensitive contrast agents for in vivo cancer imaging.…”

Section: Detection and Tracking Techniques Of Intracellular Aunpsmentioning

confidence: 99%

Detection of Intracellular Gold Nanoparticles: An Overview

D’Acunto

2018

Materials

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Photothermal therapy (PTT) takes advantage of unique properties of gold nanoparticles (AuNPs) (nanospheres, nanoshells (AuNSs), nanorods (AuNRs)) to destroy cancer cells or tumor tissues. This is made possible thanks principally to both to the so-called near-infrared biological transparency window, characterized by wavelengths falling in the range 700–1100 nm, where light has its maximum depth of penetration in tissue, and to the efficiency of cellular uptake mechanisms of AuNPs. Consequently, the possible identification of intracellular AuNPs plays a key role for estimating the effectiveness of PTT treatments. Here, we review the recognized detection techniques of such intracellular probes with a special emphasis to the exploitation of near-infrared biological transparency window.

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Cancer characterization and diagnosis with SERS-encoded particles

Cited by 61 publications

References 111 publications

Surface-Enhanced Raman Spectroscopy Analysis of Human Breast Cancer via Silver Nanoparticles: An Examination of Fabrication Methods

Surface-Enhanced Raman Spectroscopy Analysis of Human Breast Cancer via Silver Nanoparticles: An Examination of Fabrication Methods

Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies

Detection of Intracellular Gold Nanoparticles: An Overview

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