Cancer–Osteoblast Interaction Reduces Sost Expression in Osteoblasts and Up-Regulates lncRNA MALAT1 in  Prostate Cancer

Sebastian, Aimy; Hum, Nicholas R.; Hudson, Bryan D.; Loots, Gabriela G.

doi:10.3390/microarrays4040503

Cited by 31 publications

(27 citation statements)

References 69 publications

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“…MALAT1 is located at chromosome 11q13.1 and was discovered as a metastatic prognostic marker for lung cancer [ 11 ]. Also, MALAT1 has been linked to several other human tumor entities including osteosarcoma [ 16 , 33 – 38 ]. Recently, Dong found that MALAT1 could activate the PI3K/Akt pathway to promote cell proliferation, invasion and metastasis of osteosarcoma [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA MALAT1 for promoting metastasis and proliferation by acting as a ceRNA of miR-144-3p in osteosarcoma cells

et al. 2017

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Long non-coding RNAs (lncRNAs) are involved in various biological processes and diseases including osteosarcoma. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is overly expressed in osteosarcoma. But the function and mechanism it works on in osteosarcoma proliferation and metastasis mediated by Rho associated coiled-coil containing protein kinase 1 (ROCK1) and Rho associated coiled-coil containing protein kinase 2 (ROCK2) remain unclear. In the present study, an elevated MALAT1 was found in osteosarcoma tissues and cell lines, and the elevated MALAT1 was correlated with a poor prognosis in osteosarcoma patients. The functional experiments show that a decreased MALAT1 could remarkably inhibit osteosarcoma cell metastasis and proliferation but induce cell cycle arrest, indicating that MALAT1 functioned as an oncogene in osteosarcoma. Furthermore, we confirmed that MALAT1 and ROCK1/ROCK2 which were targeted by microRNA-144-3p (miR-144-3p) shared the same miR-144-3p combining site. Furthermore, the constructed luciferase assay verified that MALAT1 was a target of miR-144-3p. Additionally, the results of a qRT-PCR demonstrated that MALAT1 and miR-144-3p repressed each other's expression in a reciprocal manner. Finally, we affirmed that an overexpression of MALAT1 inhibited ROCK1/ROCK2 expression and its mediated metastasis and proliferation by working as a competitive endogenous RNA (ceRNA) via miR-144-3p.In summary, the findings of this study based on the ceRNA theory, combining the research foundation of miR-144-3p, ROCK1 and ROCK2, taking MALAT1 as a new point of study, provided new insights into molecular level proliferation reversal and metastasis of osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNA MALAT1 for promoting metastasis and proliferation by acting as a ceRNA of miR-144-3p in osteosarcoma cells

et al. 2017

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“…Another highly up-regulated lncRNA in PCa is Metastasis-associated Lung Adenocarcinoma Transcript 1 (MALAT1) that is shown to enhance EZH2-mediated repression of Polycomb-dependent target gene, E-Cadherin. Mechanistically, by interacting with the Polycomb protein enhancer of EZH2, MALAT1 is capable of facilitating EZH2 recruitment to target genes, such as E-cadherin and DAB2IP, resulting in enhanced EZH2-mediated migration and invasion in aggressive CRPC cell lines [ 142 , 143 , 144 ]. PlncRNA-1 has been shown to induce N -cadherin expression through modulating TGF-β1 signaling, and hence increase PCa cell migration and invasion motility [ 145 ].…”

Section: Long Non-coding Rna Regulation Of Emt In Pcamentioning

confidence: 99%

The Role and Mechanism of Epithelial-to-Mesenchymal Transition in Prostate Cancer Progression

Lee

et al. 2017

IJMS

100

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In prostate cancer (PCa), similar to many other cancers, distant organ metastasis symbolizes the beginning of the end disease, which eventually leads to cancer death. Many mechanisms have been identified in this process that can be rationalized into targeted therapy. Among them, epithelial-to-mesenchymal transition (EMT) is originally characterized as a critical step for cell trans-differentiation during embryo development and now recognized in promoting cancer cells invasiveness because of high mobility and migratory abilities of mesenchymal cells once converted from carcinoma cells. Nevertheless, the underlying pathways leading to EMT appear to be very diverse in different cancer types, which certainly represent a challenge for developing effective intervention. In this article, we have carefully reviewed the key factors involved in EMT of PCa with clinical correlation in hope to facilitate the development of new therapeutic strategy that is expected to reduce the disease mortality.

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“…Currently, most lncRNAs related studies focus on osteosarcoma, which is the most common primary malignant bone tumor. LncRNAs MAYA and MALAT1 have been shown to be involved in cancer cell colonization to bones. LncRNA MAYA, which acts as a scaffold, brings together LLGL2 and NSUN6 to methylate and inactivate the Hippo/MST1 pathways and is confirmed a promising therapeutic target for breast cancer bone metastasis.…”

Section: Lncrnas As Novel Players In Cancer Metastasismentioning

confidence: 99%

“…LncRNA MAYA, which acts as a scaffold, brings together LLGL2 and NSUN6 to methylate and inactivate the Hippo/MST1 pathways and is confirmed a promising therapeutic target for breast cancer bone metastasis. MALAT1 is upregulated in osteoblasts when coculturing with PC3 cells, and targeting the Sost/Wnt/MALAT1 pathway may open up new avenues of therapeutic intervention in treating prostate cancer metastasis to bones.…”

Section: Lncrnas As Novel Players In Cancer Metastasismentioning

confidence: 99%

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Molecular mechanisms of long noncoding RNAs‐mediated cancer metastasis

Egranov

Yang

et al. 2019

Genes Chromosomes & Cancer

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Cancer metastasis is a multistep process that requires cancer cells to leave the primary site, survive in the blood stream, and finally colonize at a distant organ. It is the major cause of cancer morbidity and mortality. The organ‐specific colonization requires close interaction and communication between cancer cells and host organs. Noncoding RNAs represent the majority of the transcriptome, with long noncoding RNAs (lncRNAs) making up a significant proportion. It has been suggested that lncRNAs play a key role in all stages of tumorigenesis and metastasis. This review will provide an overview of how lncRNAs are involved in cancer cell colonization in specific organ sites and the underlying mechanisms as well as therapeutic strategies.

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Cancer–Osteoblast Interaction Reduces Sost Expression in Osteoblasts and Up-Regulates lncRNA MALAT1 in Prostate Cancer

Cited by 31 publications

References 69 publications

Long non-coding RNA MALAT1 for promoting metastasis and proliferation by acting as a ceRNA of miR-144-3p in osteosarcoma cells

Long non-coding RNA MALAT1 for promoting metastasis and proliferation by acting as a ceRNA of miR-144-3p in osteosarcoma cells

The Role and Mechanism of Epithelial-to-Mesenchymal Transition in Prostate Cancer Progression

Molecular mechanisms of long noncoding RNAs‐mediated cancer metastasis

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