Cancer stem cells: a new approach to tumor development

Kobayashi, Natália Cristina Ciufa; Noronha, Samuel Marcos Ribeiro de

doi:10.1590/1806-9282.61.01.086

Cited by 15 publications

(11 citation statements)

References 44 publications

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“…The percentage of peritoneal MCAs increased proportionally to the increased concentration of chemotherapeutic agents within a 72-h period. Efficient tumorigenesis in vivo is the most important feature of cancer stem cells 22 . We selected 6 samples of peritoneal MCAs from 14 samples to perform a tumorigenicity assays in nude mice (Fig.…”

Section: Resultsmentioning

confidence: 99%

GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis

et al. 2019

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Peritoneal metastasis is one of the most important causes of postoperative death in patients with gastric cancer, and the exact mechanism remains unclear. The proliferation of multicellular aggregates of exfoliated malignant gastric cells in the abdominal cavity is the focus of current research. However, the mechanism how gastric cancer multicellular aggregates survive remains unclear. In this study, we demonstrated that multicellular aggregates of exfoliated gastric cancer cells in the abdominal cavity expressed a stem cell-Like phenotype. We found that Integrin α v β 3 not only mediated adhesion of gastric cancer multicellular aggregates to form independent functional units, but also maintained their stem cell-like phenotype by the non-classical pathway Integrin α v β 3 /ERK1/2/GLI1. In addition, ERK1/2 directly regulates the transcriptional activity of GLI1. GLI1 is a key effector of the Integrin α v β 3 pathway in regulating stem cell-like phenotype in multicellular aggregates. Our data indicates that although there is a crosstalk between the non-classical Integrin α v β 3 pathway and the classical Hedgehog pathway, the activation of GLI1 is almost independent of the Hedgehog pathway in multicellular aggregates of gastric cancer cells. Our study provides a basis for blocking GLI1 activity in the prevention and treatment of peritoneal metastases of gastric cancer.

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Section: Resultsmentioning

confidence: 99%

GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis

et al. 2019

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“…In solid tumors, tumor stem cells were detected for the first time in a breast tumor by Al-Hajj et al, 21 and exhibit characteristics of self-renewal, differentiation, increased membrane permeability, migration capacity, and antiapoptotic activity. 22,23 Thus, a specific subset of tumor cells with stem cell characteristics may play a role in tumor initiation, progression, and recurrence. 23,24,25 Tumor stem cells and associated proteins have been investigated in some oral neoplasms.…”

Section: Discussionmentioning

confidence: 99%

“…22,23 Thus, a specific subset of tumor cells with stem cell characteristics may play a role in tumor initiation, progression, and recurrence. 23,24,25 Tumor stem cells and associated proteins have been investigated in some oral neoplasms. 26 Recent studies on head and neck squamous cell carcinoma indicated an important role of tumor stem cells in tumor development and progression.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of OCT4 and CD44 in major and minor salivary gland neoplasms

et al. 2021

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The aim of this study was to identify tumor parenchyma cells exhibiting immunohistochemical profile of stem cells by evaluating the immunoreactivity of OCT4 and CD44 in a number of cases of salivary gland neoplasms. The sample consisted of 20 pleomorphic adenomas, 20 mucoepidermoid carcinomas, and 20 adenoid cystic carcinomas located in major and minor salivary glands. The expression of OCT4 and CD44 was evaluated by the percentage of positive cells and the intensity of expression. All studied cases showed positive expression of OCT4 and CD44 and higher values than the control groups. For OCT4, luminal and non-luminal cells were immunostained in the case of pleomorphic adenomas and adenoid cystic carcinomas. Moreover, the immunoreactivity of CD44 was particularly evident in the nonluminal cells of these lesions. In mucoepidermoid carcinomas, there was immunoreactivity for both markers in squamous and intermediate cells and absence of staining in mucous cells. For both markers, a significantly higher immunostaining was verified in neoplasms located in the major salivary glands compared with lesions in minor salivary glands (p<0.001). In the total sample and in minor salivary glands, malignant neoplasms exhibited higher immunoreactivity for OCT4 than pleomorphic adenoma. A significant moderate positive correlation (r = 0.444 and p ≤ 0.001) was found between OCT4 and CD44 immunoexpression in the total sample. The high expression of OCT4 and CD44 may indicate that these proteins play an important role in identifying tumor stem cells.

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“…Studies have shown that minimum amount of cancer stem cells can lead to disease relapse, and removal of cancer stem cells would mean the eradication of the disease. However, the major hurdle is to find treatments that can kill these specific stem cells without harming the normal cells[38]. The screening of cancer stem cells can be used to prognosticate the severity of OSSN and possibly its metastatic potential.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Stem Cell Markers in Ocular Surface Squamous Neoplasia

et al. 2016

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Ocular Surface Squamous Neoplasm (OSSN) is the neoplasia arising from the conjunctiva, cornea and limbus. OSSN ranges from mild, moderate, severe dysplasia, carcinoma in situ (CIS) to squamous cell carcinoma (SCC). Recent findings on cancer stem cells theory indicate that population of stem-like cell as in neoplasia determines its heterogeneity and complexity leading to varying tumor development of metastatic behavior and recurrence. Cancer stem cell markers are not much explored in the cases of OSSN. In the present study, we aim to evaluate the expression of stem cells using stem cell markers mainly p63, ABCG2, c-KIT (CD117) and CD44 in OSSN tissue, which could have prognostic significance. The present study tries for the first time to explore expression of these stem markers in the cases of OSSN. These cases are subdivided into two groups. One group comprises of carcinoma in situ (n = 6) and the second group comprises of invasive carcinoma (n = 6). The mean age at presentation was 52 years; with 53 years for CIS group and 52 years for SCC group. From each group section from the paraffin block were taken for the IHC staining of p63, c-Kit, ABCG2 and CD44. Our experiments show high expression of P63 and CD44 in the cases of CIN and SCC. Both CIS and SCC displayed positive staining with p63, with more than 80% cells staining positive. However minimal expression of c-kit in both CIN and SCC. But surprisingly we got high expression of ABCG2 in cases of carcinoma in situ as compared to that of invasive squamous cell carcinoma. More than 50% of cells showed CD44 positivity in both CIS and SCC groups. Our results show for the first time that these four stem cells especially the limbal epithelium stem cells play a vital role in the genesis of OSSN but we need to explore more cases before establishing its clinical and biological significance.

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Cancer stem cells: a new approach to tumor development

Abstract: Kobayashi NCC et al. 86rev assoC Med bras 2015; 61(1):86-93

Cited by 15 publications

References 44 publications

GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis

GLI1 activation by non-classical pathway integrin αvβ3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis

Immunohistochemical expression of OCT4 and CD44 in major and minor salivary gland neoplasms

Differential Expression of Stem Cell Markers in Ocular Surface Squamous Neoplasia

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