Cancer Stem Cells in Tumor Modeling: Challenges and Future Directions

Dogan, Elvan; Kısım, Aslı; Bati-Ayaz, Gizem; Kubicek, Gregory J.; Pesen-Okvur, Devrim; Miri, Amir K.

doi:10.1002/anbr.202170111

Cited by 6 publications

(1 citation statement)

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“…The addition of PEGDA led to more toxicity of encapsulated cells, and indeed 30%-40% v/v concentrations of PEGDA might be unsuitable for biological components. The bioink used in the fabrication processes is a potential candidate for OoCs [6,68]. The proposed PI concentration and UV exposure were found to be safe for the proposed bioink formulation.…”

Section: Microfluidic Chipsmentioning

confidence: 95%

Multi-material digital light processing bioprinting of hydrogel-based microfluidic chips

et al. 2021

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Recent advancements in digital-light-processing (DLP)-based bioprinting and hydrogel engineering have enabled novel developments in organs-on-chips. In this work, we designed and developed a multi-material, DLP-based bioprinter for rapid, one-step prototyping of hydrogel-based microfluidic chips. A composite hydrogel bioink based on poly-ethylene-glycol-diacrylate (PEGDA) and gelatin methacryloyl (GelMA) was optimized through varying the bioprinting parameters such as light exposure time, bioink composition, and layer thickness. We showed a wide range of mechanical properties of the microfluidic chips for various ratios of PEGDA:GelMA. Microfluidic features of hydrogel-based chips were then tested using dynamic flow experiments. Human-derived tumor cells were encapsulated in 3D bioprinted structures to demonstrate their bioactivity and cell-friendly environment. Cell seeding experiments then validated the efficacy of the selected bioinks for vascularized micro-tissues. Our biofabrication approach offers a useful tool for the rapid integration of micro-tissue models into organs-on-chips and high-throughput drug screening platforms.

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Section: Microfluidic Chipsmentioning

confidence: 95%