Cancer Stem Cells

Zubair, Haseeb; Azim, Shafquat; Srivastava, Sanjeev K.; Bhardwaj, Arun; Marimuthu, Saravanakumar; Patton, Mary C.; Singh, Seema; Singh, Ajay P.

doi:10.1002/9781119135449.ch20

Cited by 2 publications

(1 citation statement)

References 324 publications

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“…Cancer stem cells (CSCs) were first discovered in human acute myelogenous leukemia 1 and have since been identified in breast cancer, glioblastoma, multiple myeloma, gastric cancer, pancreatic cancer, and colon cancer, among others. 2 CSCs have an increased capacity to activate antiapoptotic and prosurvival pathways, as well as to overexpress ATP-binding cassette transporters which act as potent efflux pumps to extrude small molecules (e.g., chemotherapeutics) from the cancer cells. 3,4 As such, conventional chemotherapeutics can inadvertently lead to an enrichment of CSCs by killing non-CSCs, which in turn contributes to the emergence of highly aggressive and treatment-resistant phenotypes during relapse.…”

Section: ■ Introductionmentioning

confidence: 99%

Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1

et al. 2018

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Cancer stem cells (CSCs) are progenitor cells that contribute to treatment-resistant phenotypes during relapse. CSCs exist in specific tissue microenvironments that cell cultures and more complex models cannot mimic. Therefore, the development of new approaches that can detect CSCs and report on specific properties (e.g., stem cell plasticity) in their native environment have profound implications for studying CSC biology. Herein, we present AlDeSense, a turn-on fluorescent probe for aldehyde dehydrogenase 1A1 (ALDH1A1) and Ctrl-AlDeSense, a matching nonresponsive reagent. Although ALDH1A1 contributes to the detoxification of reactive aldehydes, it is also associated with stemness and is highly elevated in CSCs. AlDeSense exhibits a 20-fold fluorescent enhancement when treated with ALDH1A1. Moreover, we established that AlDeSense is selective against a panel of common ALDH isoforms and exhibits exquisite chemostability against a collection of biologically relevant species. Through the application of surface marker antibody staining, tumorsphere assays, and assessment of tumorigenicity, we demonstrate that cells exhibiting high AlDeSense signal intensity have properties of CSCs. Using these probes in tandem, we have identified CSCs at the cellular level via flow cytometry and confocal imaging, as well as monitored their states in animal models.

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Section: ■ Introductionmentioning

confidence: 99%

Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1

et al. 2018

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Deep-Red/Near-Infrared Turn-On Fluorescence Probes for Aldehyde Dehydrogenase 1A1 in Cancer Stem Cells

Miki

Ueda

et al. 2021

ACS Sens.

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Accumulating evidence supports that cancer stem cells (CSCs) are responsible for cancer proliferation, metastasis, and therapy resistance; therefore, an effective strategy to identify and isolate CSCs is required urgently. Because of their low invasiveness and high signal/noise ratio, “turn-on” fluorescence probes working in the deep-red/near-infrared (DR/NIR) region are one of the most attractive yet undeveloped tools for CSC detection. Herein, we report DR/NIR turn-on fluorescence probes, CS5-A and CS7-A, targeted to aldehyde dehydrogenase 1A1 as an intracellular CSC marker. In contrast to the conventional “always-on” green-fluorescent ALDEFLUOR, we succeeded in generating high-contrast (signal/noise ratio > 8.3) and wash-free in vitro CSC imaging with the DR probe C5S-A. This probe can facilitate CSC isolation with minimal contamination by autofluorescence from other tissues through fluorescence-activated cell sorting. Furthermore, the NIR absorbance/emission and turn-on properties of C7S-A allow simple and rapid CSC detection in vivo within 15 min.

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Cancer Stem Cells

Cited by 2 publications

References 324 publications

Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1

Surveillance of Cancer Stem Cell Plasticity Using an Isoform-Selective Fluorescent Probe for Aldehyde Dehydrogenase 1A1

Deep-Red/Near-Infrared Turn-On Fluorescence Probes for Aldehyde Dehydrogenase 1A1 in Cancer Stem Cells

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