Cancer Therapy Combining Modalities of Hyperthermia and Chemotherapy: in vitro Cellular Response after Rapid Heat Accumulation in the Cancer Cell

Tang, Yuan

doi:10.25148/etd.fi10081202

Cited by 1 publication

(1 citation statement)

References 108 publications

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“…48 It has been observed that when these cells are reincubated at 37°C, HSP expression drops back to basal levels with time. 21,49 However, with an increase in temperature above a distinct threshold (usually above 43ºC), cells cannot achieve any further HSP induction and the protein-folding capacity of HSPs becomes exhausted. Overload of such non-functional HSPs causes protein misfolding and aggregation, resulting in cell death.…”

Section: Effect Of Hyperthermia On Induction Of Hspsmentioning

confidence: 99%

Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3: in vitro studies

Haghniaz¹,

Umrani²,

Paknikar³

2015

IJN

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Background The purpose of this study was to investigate the therapeutic efficacy of dextran-coated (Dex) La 0.7 Sr 0.3 MnO 3 (LSMO) nanoparticles-mediated hyperthermia at different temperatures (43°C, 45°C, and 47°C) based on cell killing potential and induction of heat shock proteins in a murine melanoma cell (B16F1) line. Methods LSMO nanoparticles were synthesized by a citrate-gel method and coated with dextran. B16F1 cells were exposed to the Dex-LSMO nanoparticles and heated using a radiofrequency generator. After heating, the morphology and topology of the cells were investigated by optical microscopy and atomic force microscopy. At 0 hours and 24 hours post heating, cells were harvested and viability was analyzed by the Trypan blue dye exclusion method. Apoptosis and DNA fragmentation were assessed by terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) assay and agarose gel electrophoresis, respectively. An enzyme-linked immunosorbent assay was used to quantify heat shock protein levels. Results Our data indicate that cell death and induction of heat shock proteins in melanoma cells increased in a time-dependent and temperature-dependent manner, particularly at temperatures higher than 43°C. The mode of cell death was found to be apoptotic, as evident by DNA fragmentation and TUNEL signal. A minimum temperature of 45°C was required to irreversibly alter cell morphology, significantly reduce cell viability, and result in 98% apoptosis. Repeated cycles of hyperthermia could induce higher levels of heat shock proteins (more favorable for antitumor activity) when compared with a single cycle. Conclusion Our findings indicate a potential use for Dex-LSMO-mediated hyperthermia in the treatment of melanoma and other types of cancer.

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Section: Effect Of Hyperthermia On Induction Of Hspsmentioning

confidence: 99%