Cancer tissue screening using surface enhanced Raman scattering

Abstract

The present work summarizes our recent results in combining the FT-Raman & micro-Raman techniques with the high sensitivity of the surface enhanced Raman scattering (SERS) to characterize and monitor cancer disease in the tissue biopsy. The Raman imaging technique used for investigating the samples identified SERS signal in the Ag colloidal incubated tissues which provided a great deal of molecular information about the studied samples, indicating at the same time the chemisorption of the Ag nanoparticles inside the biological tissues. A comparison between two different set-ups, a dispersive instrument and an FT-Raman one employed for the investigation of the same samples is also presented and discussed.

A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior

“…Some of these bands were reported by previous SERS studies of cells, as well [10,34] and were assigned to amide II, tryptophan (1555 cm -1 ), CH 2 , CH 3 bending vibrations of proteins (1336 cm -1 ), proteins amide III (995 cm -1 ), CCH bending and ring breathing vibration modes of tyrosine or O-P-O stretching vibration of DNA/RNA (830 cm -1 ). Based on our previous studies focusing on the investigation of the SERS signal from tissues [36], we believe that the clearly resolved bands around 1300 cm -1 are attributable to the nucleic species.…”