Candida albicans biofilm growth and dispersal: contributions to pathogenesis

Wall, Gina; Montelongo-Jauregui, Daniel; Bonifácio, Bruna Vidal; Lopez-Ribot, José L.; Uppuluri, Priya

doi:10.1016/j.mib.2019.04.001

Cited by 186 publications

(149 citation statements)

References 44 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…Coupling constant values are given in Hz. 13 C-NMR spectra are proton decoupled. High-resolution mass spectra (HRMS) were recorded on Waters QTOF XEVO-G2 (ESI+).…”

Section: General Methods Strains and Growth Conditionsmentioning

confidence: 99%

“…Prior to the biofilm assay, yeast cultures were grown in liquid YPD medium for 24 h until the stationary phase (OD 600 [11][12][13][14][15][16][17], cells were then pelleted by centrifugation (1699 g), washed with sterile water, and cells were further inoculated into a test biofilm medium at a final concentration of 0.2 OD 600 /mL and incubated in 96-well flat-bottom polystyrene microtiter plates (Sigma-Aldrich, Corning ® Costar ® culture plates, CLS3596-50EA) for 24 or 48 h at 37 • C. At the defined time points, the biofilm developed on polystyrene was measured either by crystal violet staining as described [42,56] or by improved XTT method, where the 200 mM glucose and XTT (2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide, X4626, Sigma-Aldrich) were added to the reaction mixture [43]. The crystal violet (HT901-8FOZ, Sigma Aldrich) was added to the media at the final concentration of 0.05%.…”

Section: Biofilm Assaymentioning

confidence: 99%

“…Apart from forming biofilms on host surfaces, pathogens often form biofilms on abiotic surfaces of host implants, which brings serious implications for the use of medical devices such as prostheses, joint replacements, catheters, and pacemakers. Compared to planktonic cells, cells in biofilms are more resistant to antibiotics and antifungal drugs [1][2][3][4][5][6][7], host immune responses [8,9], and can promote further pathogen dissemination, forming new biofilms, which are associated with chronic or life threatening infections [10][11][12][13]. Thus, it is important to develop new strategies for the prevention of biofilm formation, and to eliminate already formed biofilm.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simple Carbohydrate Derivatives Diminish the Formation of Biofilm of the Pathogenic Yeast Candida albicans

et al. 2019

View full text Add to dashboard Cite

The opportunistic human fungal pathogen Candida albicans relies on cell morphological transitions to develop biofilm and invade the host. In the current study, we developed new regulatory molecules, which inhibit the morphological transition of C. albicans from yeast-form cells to cells forming hyphae. These compounds, benzyl α-l-fucopyranoside and benzyl β-d-xylopyranoside, inhibit the hyphae formation and adhesion of C. albicans to a polystyrene surface, resulting in a reduced biofilm formation. The addition of cAMP to cells treated with α-l-fucopyranoside restored the yeast-hyphae switch and the biofilm level to that of the untreated control. In the β-d-xylopyranoside treated cells, the biofilm level was only partially restored by the addition of cAMP, and these cells remained mainly as yeast-form cells.Antibiotics 2020, 9, 10 2 of 11 system. For example, the hyphal glucan consists of a unique cyclic (1-3)-linked polymer backbone with long (1-6)-linked side chains [22]. Hyphae also have less mannan, compared to yeast-form cells [23]. The morphological transition from yeast-form cells to hyphae (i.e., switching) is a complex, tightly regulated process during the biofilm maturation, which is regulated by environmental conditions and quorum sensing molecules such as farnesol and tyrosol [21]. Multiple signaling pathways such as cyclic adenosine monophosphate/protein kinase A (cAMP-PKA), high-osmolarity glycerol (HOG), mitogen-activated protein kinases (MAPK), and several transcriptional factors, Efg1, Bcr1, Ndt80, Cph1, Cph2, Tec1, Nrg1, Rfg1, and Tup1 are known to regulate the morphological transition and hyphal adhesion [1,21,[24][25][26]. Upon stimuli favoring hyphae, the yeast cells produce a germ tube that extends by polarized growth by the activity of membrane-bound vesicles supplying the components of the plasma membrane and cell wall to the growing hyphae [21].Different carbohydrates have been explored for their possibilities to disturb or prevent adhesion, biofilm formation, and the extension of an already existing biofilm of C. albicans [27][28][29][30][31][32][33][34]. In addition, some natural products of plant origin were recently shown to reduce the adhesion of C. albicans and its biofilm formation by inhibiting the yeast-to-hyphae transition [35,36].In this study, we synthesized and tested the effect of simple monosaccharides and glycosides (Chart 1) on C. albicans biofilm development on polystyrene surfaces.

show abstract

“…Coupling constant values are given in Hz. 13 C-NMR spectra are proton decoupled. High-resolution mass spectra (HRMS) were recorded on Waters QTOF XEVO-G2 (ESI+).…”

Section: General Methods Strains and Growth Conditionsmentioning

confidence: 99%

Section: Biofilm Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple Carbohydrate Derivatives Diminish the Formation of Biofilm of the Pathogenic Yeast Candida albicans

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Results revealed that the F6 fluorinated, hydrophobic copolymers performed much better in reducing the adhesion of C. albicans, with respect to both corresponding zwitterionic, hydrophilic MPC and MSA counterparts, and were similar to the glass negative control, which is well-known to inhibit the adhesion of C. albicans. A composition-dependent activity was also found, with the films of copolymer with 99 mol% F6 fluorinated co-units performing best.Polymers 2020, 12, 398 2 of 15 of sessile fungal cells, and removal of the infected device is often required [9]. However, removal of the contaminated implant can be accompanied by complications, negatively affecting the patient's condition and the economic burden.…”

mentioning

confidence: 99%

“…Polymers 2020, 12, 398 2 of 15 of sessile fungal cells, and removal of the infected device is often required [9]. However, removal of the contaminated implant can be accompanied by complications, negatively affecting the patient's condition and the economic burden.…”

mentioning

confidence: 99%

Fluorinated vs. Zwitterionic-Polymer Grafted Surfaces for Adhesion Prevention of the Fungal Pathogen Candida albicans

et al. 2020

View full text Add to dashboard Cite

Fluorinated (F6) and zwitterionic, as well as phosphorylcholine (MPC) and sulfobetaine (MSA), copolymers containing a low amount (1 and 5 mol%) of 3-(trimethoxysilyl)propyl methacrylate (PTMSi) were prepared and covalently grafted to glass slides by using the trimethoxysilyl groups as anchorage points. Glass-surface functionalization and polymer-film stability upon immersion in water were proven by contact angle and angle-resolved X-ray photoelectron spectroscopy (AR-XPS) measurements. Antifouling performance of the grafted films was assayed against the yeast Candida albicans, the most common Candida species, which causes over 80% of candidiasis. Results revealed that the F6 fluorinated, hydrophobic copolymers performed much better in reducing the adhesion of C. albicans, with respect to both corresponding zwitterionic, hydrophilic MPC and MSA counterparts, and were similar to the glass negative control, which is well-known to inhibit the adhesion of C. albicans. A composition-dependent activity was also found, with the films of copolymer with 99 mol% F6 fluorinated co-units performing best.Polymers 2020, 12, 398 2 of 15 of sessile fungal cells, and removal of the infected device is often required [9]. However, removal of the contaminated implant can be accompanied by complications, negatively affecting the patient's condition and the economic burden. Therefore, prevention of biofilm-associated infections currently represents a major challenge.The surface properties, notably the surface chemical composition, of materials susceptible to fouling are known to significantly affect the biofilm formation on medical devices [11], as well as the micro-and macro-fouler colonization of ship hulls, maritime equipment and industrial implants [12][13][14][15]. One of the most promising strategies to overcome this problem is to develop anti(bio)fouling surfaces, which prevent microorganism adhesion.The first interactions occurring among C. albicans cells and materials surfaces are usually hydrophobic interactions and electrostatic forces, taking place within the first 12 h; subsequent stages involve a stronger adhesion, displayed by cell-wall glycoproteins (e.g., Als or Hwp1 proteins) [16]. This leads to formation of microcolonies (3-4 h) and biofilm aggregates organized in a bilayer composed of yeast and hyphal cells embedded in a self-produced extracellular polymeric matrix (11-30 h). C. albicans mature biofilm is then consolidated up to 38-72 h [17].Among the different parameters that are reported to possibly affect Candida adhesion, including surface preconditioning with different biological fluids [12], surface roughness [8-11] and surface charge [9], one of the most relevant is surface wettability. It is widely acknowledged that C. albicans attaches more rapidly to hydrophobic, nonpolar surfaces, such as Teflon and other plastics, than to hydrophilic surfaces, such as glass and metals [18]. However, results of these studies have at times been contradictory, due to the absence of standardized methods for determining surfa...

show abstract

Microbial Metabolite Inspired β‐Peptide Polymers Displaying Potent and Selective Antifungal Activity

et al. 2022

View full text Add to dashboard Cite

Potent and selective antifungal agents are urgently needed due to the quick increase of serious invasive fungal infections and the limited antifungal drugs available. Microbial metabolites have been a rich source of antimicrobial agents and have inspired the authors to design and obtain potent and selective antifungal agents, poly(DL‐diaminopropionic acid) (PDAP) from the ring‐opening polymerization of β‐amino acid N‐thiocarboxyanhydrides, by mimicking ε‐poly‐lysine. PDAP kills fungal cells by penetrating the fungal cytoplasm, generating reactive oxygen, and inducing fungal apoptosis. The optimal PDAP displays potent antifungal activity with minimum inhibitory concentration as low as 0.4 µg mL−1 against Candida albicans, negligible hemolysis and cytotoxicity, and no susceptibility to antifungal resistance. In addition, PDAP effectively inhibits the formation of fungal biofilms and eradicates the mature biofilms. In vivo studies show that PDAP is safe and effective in treating fungal keratitis, which suggests PDAPs as promising new antifungal agents.

show abstract

Candida albicans biofilm growth and dispersal: contributions to pathogenesis

Cited by 186 publications

References 44 publications

Simple Carbohydrate Derivatives Diminish the Formation of Biofilm of the Pathogenic Yeast Candida albicans

Simple Carbohydrate Derivatives Diminish the Formation of Biofilm of the Pathogenic Yeast Candida albicans

Fluorinated vs. Zwitterionic-Polymer Grafted Surfaces for Adhesion Prevention of the Fungal Pathogen Candida albicans

Microbial Metabolite Inspired β‐Peptide Polymers Displaying Potent and Selective Antifungal Activity

Contact Info

Product

Resources

About