Candida albicans Biofilms Produce More Secreted Aspartyl Protease than the Planktonic Cells

Mendes, Aline; Mores, Alinne Ulbrich; Carvalho, Alessandra P.; Rosa, Rosimeire Takaki; Samaranayake, LP; Rosa, Edvaldo Antônio Ribeiro

doi:10.1248/bpb.30.1813

Cited by 51 publications

(26 citation statements)

References 10 publications

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“…In the present study, we also observed that T. inkin biofilms displayed more proteolytic activity than their planktonic counterparts. Similar results were found by Mendes et al (2007) in C. albicans biofilms. Taken together, these data suggest the importance of proteases in the development and maturation of T. inkin biofilms.…”

supporting

confidence: 89%

“…The role of proteases in fungal biofilms has recently been studied in C. albicans. Secretory aspartic proteases genes are expressed in biofilm systems in vitro (Mendes et al, 2007;Nailis et al, 2010) and it has been hypothesized that these could be important for adhesion and nutrient acquisition in mature biofilms (Nailis et al, 2010). In the present study, we also observed that T. inkin biofilms displayed more proteolytic activity than their planktonic counterparts.…”

supporting

confidence: 72%

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Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Cordeiro

Serpa

Alexandre

et al. 2015

Journal of Medical Microbiology

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The aim of this study was to determine experimental conditions for in vitro biofilm formation of clinical isolates of Trichosporon inkin, an important opportunistic pathogen in immunocompromised patients. Biofilms were formed in microtitre plates in three different media (RPMI, Sabouraud and CLED), with inocula of 10 4 , 10 5 or 10 6 cells ml 21, at pH 5.5 and 7.0, and at 35 and 28 8C, under static and shaking conditions for 72 h. Growth kinetics of biofilms were evaluated at 6, 24, 48 and 72 h. Biofilm milieu analysis were assessed by counting viable cells and quantification of nucleic acids released into biofilm supernatants. Biofilms were also analysed for proteolytic activity and antifungal resistance against amphotericin B, caspofungin, fluconazole, itraconazole and voriconazole. Finally, ultrastructural characterization of biofilms formed in microtitre plates and catheter disks was performed by scanning electron microscopy. Greater biofilm formation was observed with a starter inoculum of 10 6 cells ml 21, at pH 7.0 at 35 8C and 80 r.p.m., in both RPMI and Sabouraud media. Growth kinetics showed an increase in both viable cells and biomass with increasing incubation time, with maximum production at 48 h. Biofilms were able to disperse viable cells and nucleic acids into the supernatant throughout the developmental cycle. T. inkin biofilms produced more protease than planktonic cells and showed high tolerance to amphotericin B, caspofungin and azole derivatives. Mature biofilms were formed by different morphotypes, such as blastoconidia, arthroconidia and hyphae, in a strain-specific manner. The present article details the multicellular lifestyle of T. inkin and provides perspectives for further research.

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supporting

confidence: 89%

supporting

confidence: 72%

Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Cordeiro

Serpa

Alexandre

et al. 2015

Journal of Medical Microbiology

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“…Mutant strains in which single SAP genes are deleted are viable, as are strains with deletions of multiple SAPs (69,149,186,334,375,403). The proteinase activity in biofilm culture supernatant is greater than that in planktonic organisms (241).…”

Section: Non-cell-wall Functionsmentioning

confidence: 99%

Candida albicansCell Wall Proteins

Chaffin

2008

Microbiol Mol Biol Rev

435

404

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SUMMARY The Candida albicans cell wall maintains the structural integrity of the organism in addtion to providing a physical contact interface with the environment. The major components of the cell wall are fibrillar polysaccharides and proteins. The proteins of the cell wall are the focus of this review. Three classes of proteins are present in the candidal cell wall. One group of proteins attach to the cell wall via a glycophosphatidylinositol remnant or by an alkali-labile linkage. A second group of proteins with N-terminal signal sequences but no covalent attachment sequences are secreted by the classical secretory pathway. These proteins may end up in the cell wall or in the extracellular space. The third group of proteins lack a secretory signal, and the pathway(s) by which they become associated with the surface is unknown. Potential constituents of the first two classes have been predicted from analysis of genome sequences. Experimental analyses have identified members of all three classes. Some members of each class selected for consideration of confirmed or proposed function, phenotypic analysis of a mutant, and regulation by growth conditions and transcription factors are discussed in more detail.

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“…The main function of SAPs is to degrade proteins, but they also play a role in cell-cell adhesion [22]. [49] showed that C. albicans biofilms secrete more SAPs than do planktonic cells, while [50] showed that an in vitro C. albicans biofilm induced SAP activity, and that SAP8 expression within the biofilm correlated with in vivo denture stomatitis severity.…”

Section: Detection Of Als3 Sap5 Plb1 and Lip8 Genes In Candida Albimentioning

confidence: 99%

Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

2017

ijs

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Biofilm formation (BF) is one of the most important virulence factors of Candida spp. The aim of this study was to detect the prevalence of genes responsible in biofilm formation of C. albicans by conventional PCR technique. Among 49 vaginal specimens (VC), C. albicans was the most predominant species in percentage 22/49 (45%) and 27(55%) were non albicans. Out of 47 oral specimens (OS), 22/47(47%) were C. albicans, whereas 25(53%) were non albicans. At the present study; all C. albicans were biofilm producers with variable strength, out of 44 BF producers, 18 (40.9%) were low biofilm (LBF) with significant differences (P<0.05) between HVS and OS, 25 (56.8%) moderate or high biofilm (HBF) and just one isolate from oral was strong (very high) biofilm in percentage about 2.3% with no significant differences between HVS and OS.Molecular study of virulence genes correlated with biofilm C. albicans show that HWP1, ALS1, ALS3, SAP5, PLB1 and LIP8 genes were detected in 100% of C. albicans from vaginal and oral infections. Keywords ISSN: 0067-2904Iraqi Journal of Science, 2017, Vol. 58, No.2C, pp: 988-

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Candida albicans Biofilms Produce More Secreted Aspartyl Protease than the Planktonic Cells

Cited by 51 publications

References 10 publications

Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals

Candida albicansCell Wall Proteins

Detection The Prevalence of Adhesins and Extracellular hydrolytic enzymes genes in Candida albicans Biofilm Formation

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