Candida albicans cellular internalization: a new pathogenic factor?

Drago, Lorenzo; Mombelli, B.; Vecchi, Elena De; Bonaccorso, C.; Fassina, Maria Cristina; Gismondo, Maria Rita

doi:10.1016/s0924-8579(00)00296-x

Cited by 34 publications

(41 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…albicans (14), while others have shown that epithelial cells are unable to phagocytize particles greater than 1.0 m in diameter (32), which is approximately half the size of the blastoconidia form of C. albicans. Our studies found no evidence of phagocytosis.…”

Section: Discussionmentioning

confidence: 99%

Potential Role for a Carbohydrate Moiety in Anti-CandidaActivity of Human Oral Epithelial Cells

et al. 2001

View full text Add to dashboard Cite

Candida albicans is both a commensal and a pathogen at the oral mucosa. Although an intricate network of host defense mechanisms are expected for protection against oropharyngeal candidiasis, anti-Candida host defense mechanisms at the oral mucosa are poorly understood. Our laboratory recently showed that primary epithelial cells from human oral mucosa, as well as an oral epithelial cell line, inhibit the growth of blastoconidia and/or hyphal phases of several Candida species in vitro with a requirement for cell contact and with no demonstrable role for soluble factors. In the present study, we show that oral epithelial cell-mediated anti-Candida activity is resistant to gamma-irradiation and is not mediated by phagocytosis, nitric oxide, hydrogen peroxide, and superoxide oxidative inhibitory pathways or by nonoxidative components such as soluble defensin and calprotectin peptides. In contrast, epithelial cell-mediated anti-Candida activity was sensitive to heat, paraformaldehyde fixation, and detergents, but these treatments were accompanied by a significant loss in epithelial cell viability. Treatments that removed existing membrane protein or lipid moieties in the presence or absence of protein synthesis inhibitors had no effect on epithelial cell inhibitory activity. In contrast, the epithelial cell-mediated anti-Candida activity was abrogated after treatment of the epithelial cells with periodic acid, suggesting a role for carbohydrates. Adherence of C. albicans to oral epithelial cells was unaffected, indicating that the carbohydrate moiety is exclusively associated with the growth inhibition activity. Subsequent studies that evaluated specific membrane carbohydrate moieties, however, showed no role for sulfated polysaccharides, sialic acid residues, or glucose-and mannose-containing carbohydrates. These results suggest that oral epithelial cell-mediated anti-Candida activity occurs exclusively with viable epithelial cells through contact with C. albicans by an as-yet-undefined carbohydrate moiety.

show abstract

Section: Discussionmentioning

confidence: 99%

Potential Role for a Carbohydrate Moiety in Anti-CandidaActivity of Human Oral Epithelial Cells

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Slime production was observed by scanning electron microscopy (SEM): after bacterial incubation for 18 h on glass slides with or without subinhibitory concentrations of antibiotics, the slides were removed and processed as described elsewhere (6).…”

mentioning

confidence: 99%

Effect of Linezolid in Comparison with That of Vancomycin on Glycocalix Production: In Vitro Study

Drago

Vecchi

Valli

et al. 2002

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

“…An increased prevalence of microbial resistance to commercial antifungal drugs has been noted (Drago et al 2000). Low therapeutic efficacy observed in some infections could be due to the poor penetration of the drugs into infected cells, preventing the drug from reaching the yeast (Drago et al 2000). All these aspects make the search for new antimicrobial compounds important, including those that may be present in plant extracts (Filoche et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…In the search of plant extracts with antimicrobial activity, it is very important to show that they are not toxic to these cells, which should be included as a control in the screening procedure. An interesting strategy for the discovery and development of novel compounds with antimycotic activity is based on the search for compounds that interfere with the adhesion process of C. albicans to host cells (Drago et al 2000). In this context, the study of extracts from medicinal plants used to treat infections is attractive.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory effect of extracts from Brazilian medicinal plants on the adhesion of Candida albicans to buccal epithelial cells

Johann

Silva

Martins

et al. 2008

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Plants are known to produce a plethora of secondary metabolites which are recognized as a useful source of new drugs or drug leads. Extracts and fractions of Schinus terebinthifolius Raddi (Anacardiaceae), Piper regnellii C.D.C. (Piperaceae), Rumex acetosa L. (Polygonaceae), and Punica granatum L. (Punicaceae) were assessed for their antifungal activity against eight clinical isolates of C. albicans. They were also evaluated for their effect on the adhesion of these C. albicans isolates to buccal epithelial cells (BECs). The ethyl acetate fraction from the leaves of S. terebinthifolius showed promising activity, inhibiting the growth of three C. albicans isolates at 7.8 lg ml -1 and significantly inhibiting their adhesion to BEC at 15 lg ml -1 . In addition, this fraction did not show cytotoxic activity against murine macrophages. The results show the potential of the plant extracts studied as a source of new antifungal compounds. Further studies are necessary for isolation and characterization of the active compounds of these plants.

show abstract

Candida albicans cellular internalization: a new pathogenic factor?

Cited by 34 publications

References 6 publications

Potential Role for a Carbohydrate Moiety in Anti-CandidaActivity of Human Oral Epithelial Cells

Potential Role for a Carbohydrate Moiety in Anti-CandidaActivity of Human Oral Epithelial Cells

Effect of Linezolid in Comparison with That of Vancomycin on Glycocalix Production: In Vitro Study

Inhibitory effect of extracts from Brazilian medicinal plants on the adhesion of Candida albicans to buccal epithelial cells

Contact Info

Product

Resources

About