Candida albicans Infections: a novel porcine wound model to evaluate treatment efficacy

Abstract

Candida albicans is a common cause of opportunistic mycoses worldwide and a major contributor in wound infections. The purpose of this study was to establish a fungal wound model and analyze the effects of a common antifungal agent against the proliferation of three C. albicans strains. Second degree burns were created, and then inoculated with one of three different C. albicans ATCC strains: 10261 reference strain, 64550 fluconazole resistant and 26310 fluconazole sensitive. After fungal inoculation, every wound was covered with dressings for 4 h to allow fungal colonization on every wound bed. After 4 h, the dressings were removed, and each wound was treated either once or twice daily with a topical terbinafine hydrochloride or left untreated. On days 2, 4 and 7 post inoculation, three wounds from each treatment group were scrub cultured and quantified. On day 2, wounds infected with the sensitive strains 26310 and 10261 and treated twice showed a significant reduction when compared against those infected wounds receiving once daily treatment. On day 4, wounds which were infected with C. albicans fluconazole sensitive (ATCC 26310) showed a significant reduction in fungal cell counts with treatment applied twice daily. A significant reduction in the colony counts was exhibited in all three strains at the seventh day with active as compared to the non-treated wounds. Twice daily treatment resulted in a lower fungal count than once daily treatment. Neither treatment was able to entirely eradicate C. albicans during the duration of this study. Establishing a reliable fungal wound model will help in the translational goal of identifying new antifungal that could be used clinically by wound care providers.

Propolis-Loaded Poly(lactic-co-glycolic Acid) Nanofibers: An In Vitro Study

“…Developing an effective wound dressing against C. albicans is crucial for the treatment of life-threatening infections in burn patients . Our results indicated that nanofibers containing 50 and 100% propolis can be effective when used as potential wound dressings in S.…”

Quercetin‐loaded sodium alginate/collagen/h‐boron nitride potential wound dressings prepared using the Box‐Behnken experimental design

“…Recent studies have shown that C. albicans is a 22% infectious agent in chronic wounds. [ 79 ] Within the scope of the study, the antimicrobial activity of the membrane, which gave the best results, on pathogens found at risk in wound sites was investigated. While no antimicrobial activity was observed on gram negative pathogens ( E. coli , E. aerogenes and K. pneumonia ), high activity was determined on S. aureus (21.5 mm) and C. albicans (16.0 mm) (Table 6 and Figure 11).…”

Our current clinical understanding of Candida biofilms: where are we two decades on?

“…Drosophila melanogaster , Caenorhabditis elegans , and Galleria mellonella) have been applied to candidal biofilm studies, each of them having advantages and disadvantages (172). There are a significant number of studies using mammalian animal models, such as the rat indwelling catheter model (8) and a rabbit catheter model (173), in addition to porcine wound models (174). While these are all immensely useful, they are costly and can prohibit progress in evaluating new innovative therapies.…”