Candida albicans isolated from urine: Phenotypic and molecular identification, virulence factors and antifungal susceptibility

Wiebusch, Laura; Almeida-Apolonio, Adriana Araújo de; Rodrigues, Luana Mireli Carbonera; Bicudo, Bruna de Paula; Silva, Danielly Beraldo dos Santos; Lonchiati, Danielle Ferreira; Araújo, Renata Pires de; Barufatti, Alexéia; Oliveira, Kelly Mari Pires de

doi:10.1016/j.apjtb.2017.06.006

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…showed 100% sensitivity to amphotericin-B [24]. Similarly, in this study, C krusei showed 100% resistance to uconazole similar to the study conducted by Ravinder et al [3].…”

Section: Discussionsupporting

confidence: 89%

Study of Candida species in various clinical specimens at UCMS-TH, Bhairahawa, Nepal

Ghimire¹,

Reddy

Raut³

2023

Preprint

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Introduction: The incidence of fungal infections has increased significantly in the recent times contributing to high rates of morbidity and mortality. Although being the normal flora of various parts of human body, Candida species are recognized as a major fungal pathogen causing both superficial and deep-seated infection in immunosuppressed patients. Increase in antimicrobial resistance recently has rendered treatment difficult due to restricted number of antifungal drugs. Methods: This is a descriptive cross-sectional study done for a period of 18 months at a tertiary care center. Ethical clearance was obtained from Institutional Review Committee with a referece number (UCMS/IRC/036/18). Two hundred isolates of Candida species were identified from various clinical samples by using phenotypic tests such as CHROM agar, sugar fermentation test, sugar assimilation test and germ tube test. Antifungal susceptibility test was performed for five drugs namely ketoconazole (10µg), fluconazole (10µg), itraconazole (10µg), nystatin (100µg) and amphotericin-B(20µg). All the statistical evaluation was done by using SPSS version 20.0 software (IBM Corp., Armonk, NY). Results: Among 200 isolates of Candida, the most frequently isolated species was C.albicanswhich was seen in 69% of total isolates followed by C.tropicalis, C.krusei, and C.dubliniensis. Maximum Candida isolates were from urine sample (41.5%) followed by sputum (22.5%). Amphotericin –B was found to be the most sensitive drug with a sensitivity of 97.1% whereas ketoconazole was the least sensitive drug with a sensitivity of 40.5% among the isolates. Conclusion: C.albicans were the major isolates in this study, however, there is an increased incidence of non- albicans Candida species. Antifungal susceptibility test revealed increased resistance to different antifungal drugs among Candida species. This may conclude that increase in resistant strains may lead to difficult management of Candida infection as there are very few antifungal agents available.

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“…showed 100% sensitivity to amphotericin-B [24]. Similarly, in this study, C krusei showed 100% resistance to uconazole similar to the study conducted by Ravinder et al [3].…”

Section: Discussionsupporting

confidence: 89%

Study of Candida species in various clinical specimens at UCMS-TH, Bhairahawa, Nepal

Ghimire¹,

Reddy

Raut³

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The plates were incubated for 48 h at 37°C. The results expressing the enzyme activity were interpreted as follows: Pz = 1 (no enzyme activity), 0.63 < Pz < 1.0 (moderate enzyme activity), and Pz < 0.63 (strong enzymatic activity) (Wiebusch et al 2017).…”

Section: Methodsmentioning

confidence: 99%

Resensitization of Fluconazole-Resistant Urinary Candida spp. Isolates by Amikacin through Downregulation of Efflux Pump Genes

Edward

Mohamed

Zakaria

2020

Polish Journal of Microbiology

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A b s t r a c tThe contribution of fluconazole-resistant Candida spp. isolates to urinary tract infections in Egypt has become a nationwide problem. A recent approach to overcome such disaster is combining conventional antifungals with non-antifungals. This study investigated the interaction of amikacin with fluconazole against resistant Candida strains isolated from the urine culture of patients admitted to Alexandria Main University Hospital. Among the collected Candida spp. isolates, 42.9% were resistant to fluconazole with MICs ranging between 128 and 1,024 µg/ml. The resistance-modifying activity of amikacin (4,000 µg/ml) was studied against fluconazole-resistant isolates where amikacin sensitized 91.7 % of resistant Candida spp. isolates to fluconazole with a modulation factor ranging between 32 and 256. The rhodamine efflux assay was performed to examine the impact of amikacin on efflux pump activity. After 120 minutes of treatment, amikacin affected the efflux pump activity of the isolates tested with a percentage of reduction in the fluorescence intensity of 8.9%. Quantitative real-time PCR was applied to assess the amikacin effect on the expression of the efflux pump genes MDR1, CDR1, and CDR2. The downregulatory effect of amikacin on the expression of the studied genes caused a percentage of reduction in the expression level ranging between 42.1 and 94%. In conclusion, amikacin resensitized resistant Candida spp. isolates to fluconazole and could be used in combination in the management of candiduria with a higher efficiency or at lower administration doses. To the best of our knowledge, this is the first study evaluating the enhancement of fluconazole activity in combination with amikacin against Candida spp. K e y w o r d s: Candida spp., amikacin, fluconazole, rhodamine efflux assay, quantitative real-time PCR Edward E.A. et al.

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Antifungal activity of aminoalcohols and diamines against dermatophytes and yeast

et al. 2020

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Candida albicans isolated from urine: Phenotypic and molecular identification, virulence factors and antifungal susceptibility

Cited by 5 publications

References 24 publications

Study of Candida species in various clinical specimens at UCMS-TH, Bhairahawa, Nepal

Study of Candida species in various clinical specimens at UCMS-TH, Bhairahawa, Nepal

Resensitization of Fluconazole-Resistant Urinary Candida spp. Isolates by Amikacin through Downregulation of Efflux Pump Genes

Antifungal activity of aminoalcohols and diamines against dermatophytes and yeast

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