Candida albicans Msi3p, a homolog of the Saccharomyces cerevisiae Sse1p of the Hsp70 family, is involved in cell growth and fluconazole tolerance

Nagao, Jun Ichi; Cho, Tamaki; Uno, Jun; Ueno, Keigo; Imayoshi, Rieko; Nakayama, Hironobu; Chibana, Hiroji; Kaminishi, Hidenori

doi:10.1111/j.1567-1364.2012.00822.x

Cited by 21 publications

(23 citation statements)

References 38 publications

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“…aureus staphopain B were found in haemolymph 24 h post-infection. SSA1 can induce host cell endocytosis of C. albicans , which leads to increased virulence [70] and plays a role in resistance to antimicrobial peptides and antifungal agents [71–73]. Peroxiredoxin TSA1-A was also detected in larvae mono-infected with C. albicans [39] and plays a role in cell protection against oxidative stress [74].…”

Section: Discussionmentioning

confidence: 99%

Candida albicans increases the pathogenicity of Staphylococcus aureus during polymicrobial infection of Galleria mellonella larvae

2020

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This study detailed the responses of Galleria mellonella larvae to disseminated infection caused by co-infection with Candida albicans and Staphylococcus aureus . Doses of C. albicans (1×105 larva−1) and S. aureus (1×104 larva−1) were non-lethal in mono-infection but when combined significantly (P<0.05) reduced larval survival at 24, 48 and 72 h relative to larvae receiving S. aureus (2×104 larva−1) alone. Co-infected larvae displayed a significantly higher density of S. aureus larva−1 compared to larvae infected solely with S. aureus . Co-infection resulted in dissemination throughout the host and the appearance of large nodules. Co-infection of larvae with C. albicans and S. aureus (2×104 larva−1) resulted in an increase in the density of circulating haemocytes compared to that in larvae infected with only S. aureus . Proteomic analysis of co-infected larval haemolymph revealed increased abundance of proteins associated with immune responses to bacterial and fungal infection such as cecropin-A (+45.4-fold), recognition proteins [e.g. peptidoglycan-recognition protein LB (+14-fold)] and proteins associated with nodule formation [e.g. Hdd11 (+33.3-fold)]. A range of proteins were also decreased in abundance following co-infection, including apolipophorin (−62.4-fold), alpha-esterase 45 (−7.7-fold) and serine proteinase (−6.2-fold). Co-infection of larvae resulted in enhanced proliferation of S. aureus compared to mono-infection and an immune response showing many similarities to the innate immune response of mammals to infection. The utility of G. mellonella larvae for studying polymicrobial infection is highlighted.

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Section: Discussionmentioning

confidence: 99%

Candida albicans increases the pathogenicity of Staphylococcus aureus during polymicrobial infection of Galleria mellonella larvae

2020

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“…Minimum inhibitory concentration (MIC) of micafungin for the contPHR1 strain at 37ºC based on E-test was 0.032 µg/ml. Concentrations of micafungin were used based on the MIC value and our previous report 25) . The plates were incubated at 37ºC or 39ºC for 36 hr.…”

Section: Spot Assaymentioning

confidence: 99%

“…Although the outer cell wall thickness did not change between cells grown at 37ºC and 39ºC, the inner cell wall at 39ºC was significantly thicker compared to 37ºC (P < 0.01). According to a previous paper, the outer cell wall is the mannan fibrillar layer and the inner cell wall is the chitin and β-glucans layer 25) . Therefore, mild heat stress leads to decrease in electron density of the mannan fibrillar layer and to increase in thickness of the chitin and β-glucans layer.…”

Section: Mild Heat Stress Extends the Period Of Hyphal Maintenance Inmentioning

confidence: 99%

Mild Heat Stress Affects on the Cell Wall Structure in <i>Candida albicans</i> Biofilm

Ikezaki

Cho

Nagao

et al. 2019

Medical Mycology Journal

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We previously reported that Candida albicans responded to mild heat stress in a range of temperature elevations simulating fever, and concluded that mild heat stress increases susceptibility to antifungal drugs. In this study, we show that mild heat stress causes a morphological change in hyphae during the process of biofilm formation. We found that mild heat stress extended the period of hyphal stage maintenance in C. albicans biofilm. Although the rate of hyphal change from yeast form to hyphal form reached the maximum within 3 hr, later, almost every cell quickly reverted to the yeast growth phase within 6 hr at 37ºC but not at 39ºC, or under mild heat stress. Electron microscopy using a smart specimen preparation technique revealed that mild heat stress significantly increased the thickness of the inner cell wall accompanied by a decrease in density of the outer cell wall in the hyphae of C. albicans biofilm. To identify the gene responsible for the morphological changes associated with mild heat stress, we performed microarray gene expression analysis. Eleven genes were upregulated and 17 genes were downregulated under mild heat stress in biofilm cells. The increased PHR1 gene expression in response to mild heat stress was confirmed in quantitative RT-PCR analysis. The mutant upregulated PHR1 expression showed the same sensitivity against antifungal drug micafungin as dependent on mild heat stress. Our findings point to possible therapeutic effects of hyperthermia as well as to the effect of fever during infections.

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“…Hsps are triggered by various stressors and initiate adaptation mechanisms in response to cellular stress as well as protein folding processes (14). Unlike Hsp90, Hsp70 proteins promiscuously interact with all proteins in their unfolded, misfolded, or aggregated forms.…”

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confidence: 99%

Blocking Hsp70 Enhances the Efficiency of Amphotericin B Treatment against Resistant Aspergillus terreus Strains

Blatzer

Blum

Jukic

et al. 2015

Antimicrob Agents Chemother

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The polyene antifungal amphotericin B (AmB) is widely used to treat life-threatening fungal infections. Even though AmB resistance is exceptionally rare in fungi, most Aspergillus terreus isolates exhibit an intrinsic resistance against the drug in vivo and in vitro. Heat shock proteins perform a fundamental protective role against a multitude of stress responses, thereby maintaining protein homeostasis in the organism. In this study, we elucidated the role of heat shock protein 70 (Hsp70) family members and compared resistant and susceptible A. terreus clinical isolates. The upregulation of cytoplasmic Hsp70 members at the transcriptional as well as translational levels was significantly higher with AmB treatment than without AmB treatment, particularly in resistant A. terreus isolates, thereby indicating a role of Hsp70 proteins in the AmB response. We found that Hsp70 inhibitors considerably increased the susceptibility of resistant A. terreus isolates to AmB but exerted little impact on susceptible isolates. Also, in in vivo experiments, using the Galleria mellonella infection model, cotreatment of resistant A. terreus strains with AmB and the Hsp70 inhibitor pifithrin-resulted in significantly improved survival compared with that achieved with AmB alone. Our results point to an important mechanism of regulation of AmB resistance by Hsp70 family members in A. terreus and suggest novel drug targets for the treatment of infections caused by resistant fungal isolates.

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Candida albicans Msi3p, a homolog of the Saccharomyces cerevisiae Sse1p of the Hsp70 family, is involved in cell growth and fluconazole tolerance

Cited by 21 publications

References 38 publications

Candida albicans increases the pathogenicity of Staphylococcus aureus during polymicrobial infection of Galleria mellonella larvae

Candida albicans increases the pathogenicity of Staphylococcus aureus during polymicrobial infection of Galleria mellonella larvae

Mild Heat Stress Affects on the Cell Wall Structure in <i>Candida albicans</i> Biofilm

Blocking Hsp70 Enhances the Efficiency of Amphotericin B Treatment against Resistant Aspergillus terreus Strains

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