Candida albicans SC5314 inhibits NLRP3/NLRP6 inflammasome expression and dampens human intestinal barrier activity in Caco-2 cell monolayer model

Mao, Xiaqiong; Qiu, Xinyun; Jiao, Chunhua; Lu, Meijiao; Zhao, Xiaojing; Li, Xueting; Li, Jiajia; Ma, Jingjing; Zhang, Hongjie

doi:10.1016/j.cyto.2019.154882

Cited by 21 publications

(13 citation statements)

References 30 publications

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“…16,19 Within the Candida genus, the most abundant member in healthy individuals and IBD patients is C. albicans, which has the greatest clinical relevance in humans. 39 Aspergillus spores are ubiquitous and many Aspergillus species, such as Aspergillus flavus, Aspergillus fumigatus, and Aspergillus niger, can cause major invasive diseases. 40 The gastrointestinal tract may represent a portal of entry for Aspergillus species in immunocompromised patients.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of fungal and bacterial dysbiosis in young adult Chinese patients with Crohn’s disease

Qiu

Zhao

Cui

et al. 2020

Therap Adv Gastroenterol

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Intestinal microbiota dysbiosis has been described in inflammatory bowel disease (IBD), but data from China are limited. In this study, we performed molecular analysis of the fecal microbial community from 20 healthy Chinese subjects and 25 patients with Crohn’s disease (CD), and evaluated associations with bacterial and fungal compositions. Decreased richness and diversity of bacterial composition was observed in the CD group compared with healthy (H) subjects. Significant structural differences in bacterial (but not fungal) composition among healthy controls and CD patients were found. A reduction in Firmicutes and Actinobacteria abundance, and overrepresentation of Proteobacteria were observed in the CD patients compared with the H group. The Escherichia-Shigella genus was overrepresented in the CD group, whereas Faecalibacterium, Gemmiger, Bifidobacterium, Romboutsia, Ruminococcus, Roseburia, and Fusicatenibacter abundance were decreased in the CD group compared with H subjects. Differences in fungal microbiota between the H and CD groups were observed at the genus rather than at the phylum level. The Candida genus was overrepresented in the CD (active disease) group compared with the H group, whereas no difference between CD (remission) and H groups was observed. Aspergillus, unclassified_Sordariomycetes, and Penicillium genera had greater representation in the H subjects compared with the CD group. Bacterial and fungal intra- and inter-kingdom correlations were observed between the H and CD groups. Therefore, fecal bacterial and fungal microbiome communities differed considerably between H and CD patients, and between Chinese and Western populations. The role of gut microbiota in homeostasis and in gastrointestinal disorders should be investigated further.

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Section: Discussionmentioning

confidence: 99%

“…16,19 Within the Candida genus, the most abundant member in healthy individuals and IBD patients is C. albicans , which has the greatest clinical relevance in humans. 39…”

Section: Discussionmentioning

confidence: 99%

Characterization of fungal and bacterial dysbiosis in young adult Chinese patients with Crohn’s disease

Qiu

Zhao

Cui

et al. 2020

Therap Adv Gastroenterol

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“…First, we compared cell origins. In TEER evaluation, a widely accepted quantitative technique to measure the integrity of tight junction dynamics in cell culture models [ 41 , 42 , 43 ], the AD-MSC coculture group exhibited a significant increase in TEER values compared with the BM-MSC coculture and non-coculture groups. In addition, AD-MSCs are relatively easy to collect, and a large number of cells can be obtained within a short period of time.…”

Section: Discussionmentioning

confidence: 99%

Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells

et al. 2021

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With the emergence of coronavirus disease-2019, researchers have gained interest in the therapeutic efficacy of mesenchymal stem/stromal cells (MSCs) in acute respiratory distress syndrome; however, the mechanisms of the therapeutic effects of MSCs are unclear. We have previously reported that adipose-derived MSCs (AD-MSCs) strengthen the barrier function of the pulmonary vessels in scaffold-based bioengineered rat lungs. In this study, we evaluated whether AD-MSCs could enhance the intercellular barrier function of lung epithelial cells in vitro using a transwell coculture system. Transepithelial electrical resistance (TEER) measurements revealed that the peak TEER value was significantly higher in the AD-MSC coculture group than in the AD-MSC non-coculture group. Similarly, the permeability coefficient was significantly decreased in the AD-MSC coculture group compared to that in the AD-MSC non-coculture group. Immunostaining of insert membranes showed that zonula occuldens-1 expression was significantly high at cell junctions in the AD-MSC coculture group. Moreover, cell junction-related gene profiling showed that the expression of some claudin genes, including claudin-4, was upregulated in the AD-MSC coculture group. Taken together, these results showed that AD-MSCs enhanced the barrier function between lung epithelial cells, suggesting that both direct adhesion and indirect paracrine effects strengthened the barrier function of lung alveolar epithelium in vitro.

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“…C. albicans is a polymorphic fungal pathogen which colonizes the human gastrointestinal mucosal tissues, and has effects on the intestinal mucosal barrier (17). C. albicans can serve as a pathogen in various infections and cause mucosal diseases (18).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the potential mechanism of farnesol in protecting the intestinal epithelium barrier from invasion by Candida albicans via untargeted metabolomics

Wu¹,

Yin²,

Y³

et al. 2021

Ann Palliat Med

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Background: This study aimed to explore the potential mechanisms of farnesol in the protection of the intestinal epithelium barrier from invasion by Candida albicans (C. albicans) via untargeted metabolomics.Methods: The C. albicans reference strain SC5314 and Caco-2 cells were used in this study. The effect of different concentrations of farnesol on the co-culture of C. albicans and Caco-2 cells was investigated using the CCK-8 assay. The effect of farnesol on C. albicans biofilm formation was also observed. There were 4 treatment groups, including the Caco-2 + C. albicans (group 1), Caco-2 (group 2), Caco-2 + C. albicans + farnesol (group 3), and a quality control (QC group) for metabolite extraction, followed by LC-MS/MS analysis and bioinformatics analysis.Results: Farnesol treatment significantly reduced the adhesion of C. albicans and inhibited the formation of C. albicans biofilm. A total of 22 differential metabolites were identified in group 1 vs. group 2, such as acetylcarnitine, linoleic acid, spermidine, and glutathione disulfide. These differential metabolites were involved in fatty acid biosynthesis, linoleic acid metabolism, biosynthesis of unsaturated fatty acids, and glutathione metabolism. There were 18 differential metabolites identified in group 3 vs. group 1, including acetylcarnitine, hypoxanthine, L-glutamate, and linoleic acid, which were enriched in fatty acid biosynthesis, linoleic acid metabolism, and biosynthesis of unsaturated fatty acids.Conclusions: C. albicans can damage the intestinal barrier by affecting the metabolism of acetylcarnitine, linoleic acid, glutathione. Farnesol may protect the intestinal epithelium barrier from the invasion of C. albicans by regulating the metabolism of acetylcarnitine, linoleic acid, and L-glutamate.

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Candida albicans SC5314 inhibits NLRP3/NLRP6 inflammasome expression and dampens human intestinal barrier activity in Caco-2 cell monolayer model

Cited by 21 publications

References 30 publications

Characterization of fungal and bacterial dysbiosis in young adult Chinese patients with Crohn’s disease

Characterization of fungal and bacterial dysbiosis in young adult Chinese patients with Crohn’s disease

Increased In Vitro Intercellular Barrier Function of Lung Epithelial Cells Using Adipose-Derived Mesenchymal Stem/Stromal Cells

Investigation of the potential mechanism of farnesol in protecting the intestinal epithelium barrier from invasion by Candida albicans via untargeted metabolomics

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