Candida Biofilm Modeling: Past and Present

Sachivkina, Nadezhda; Lenchenko, Ekaterina; Маннапова, Р. Т.; Strizhakov, Alexander; Romanova, E.V.; Lukina, D.M.

doi:10.29296/25419218-2019-03-03

Cited by 12 publications

(12 citation statements)

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“…In recent years, there has been a trend in the growth of candidiasis among people and animals. Fungi of the genus Candida are also very frequent participants in microbial associations [12][13][14][15].…”

Section: Discussionmentioning

confidence: 99%

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Sachivkina

Podoprigora

2021

Vet World

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Background and Aim: Different Candida species isolated in humans and animals have different types of parasite activity. The most pathogenic species is Candida albicans followed by Candida tropicalis. However, the effects of the morphology of Candida krusei, Candida guilliermondii, and Candida glabrata biofilms on the pathogenicity of these species have not been fully characterized. To the best of our knowledge, there is no literature on the effect of farnesol on rare Candida species. This study aimed to check the effect of different farnesol concentrations on the species C. krusei, C. guilliermondii, and C. glabrata compared with the strain C. albicans ATCC 10231, which has been widely studied, and is a strong producer of biofilms. Materials and Methods: We studied the morphological and densitometric parameters of biofilms produced by Candida species under the influence of the drug farnesol (Sigma-Aldrich, St. Louis, MO). We used a heart brain broth with the addition of 2% bovine blood serum in 96-well plates. To each well, we added 100 μL of C. albicans, C. krusei, C. guilliermondii, or C. glabrata culture, and 0.2-400 μM farnesol. The microliter plates were cultured with the lid closed at 37°C for 48 h. Then, the liquid was removed, and the wells were washed 3 times with 200 μL phosphate buffer solution (pH 7.3). Biofilm fixation was performed using 150 μL of 96% ethanol for 15 min. Then, the microliter plates were dried for 20 min at 37°C, a 0.5% solution of crystalline violet was added, and the plates were placed in an incubator at 37°C. After 5 min, the contents of the wells were removed, washed 3 times with 200 μL of phosphate buffer solution (pH 7.2), and dried. The dye was extracted by washing with 200 μL of 96% ethanol for 30 min. The results were obtained using a photometric analyzer of enzyme immunoassay reactions at an optical density (OD) wavelength of 450 nm. Results: All of Candida spp. strains tested were susceptible to farnesol at concentrations ranging from 0.8 to 400 μM for C. albicans, C. krusei, and C. guilliermondii, and 12.5 to 400 μM for C. glabrata. Conclusion: This study provides new insights into the use of farnesol against biofilms produced by Candida species, but further studies in vivo are necessary to evaluate the effectiveness of the reduction of OD. To the best of our knowledge, the antimicrobial activity of farnesol against C. krusei, C. guilliermondii, and C. glabrata has not been reported previously, although studies have confirmed the inhibitory effect of farnesol on the growth of different microorganisms.

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Section: Discussionmentioning

confidence: 99%

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Sachivkina

Podoprigora

2021

Vet World

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“…Tubes were incubated for 1 h at 37 °C under constant shaking. After interaction with farnesol, YLF was washed three times with PhS [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

Reduction in Pathogenicity in Yeast-like Fungi by Farnesol in Quail Model

Sachivkina

Vasilieva

Lenchenko

et al. 2022

Animals

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Candida albicans was the first eukaryotic microorganism to exhibit quorum-sensing through the secretion of the sesquiterpene E, farnesol. This molecule is generated by dephosphorylation of farnesyl pyrophosphate in the mevalonate biosynthetic pathway in mammalian and yeast cells. Exogenous farnesol inhibits yeast-to-hyphal formation in a concentration- and time-dependent manner at the earliest stage of hyphal development. Much research has been devoted to studying the role of farnesol as an inhibitor of hyphal morphogenesis; however, little research has been published regarding the in vivo impacts of farnesol on fungal virulence and the development of Candida infection. While other studies have examined the impact of multiple doses of farnesol in addition to antimycotics, we hypothesize that C. albicans treated with a single dose of this quorum-sensing molecule could reduce fungal virulence in a quail model.

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“…For pathogenic microorganisms to survive in the conditions of the biotope, they must acquire certain Available at www.veterinaryworld.org/Vol.13/January-2020/29.pdf properties, including the formation of biofilms [5][6][7]. As a result, they subsequently acquire the ability to resist the factors of natural forces such as some macroorganisms and antimicrobial agents of various origins [8].…”

Section: Introductionmentioning

confidence: 99%

Morphological and adhesive properties of Klebsiella pneumoniae biofilms

et al. 2020

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Background and Aim: The study of biofilm-forming ability of Gram-negative microflora has great practical importance for assessing the effectiveness of antibiotic therapy and finding new ways to diagnose and inhibit the growth of biofilms. This is because poor penetration of antibacterial drugs into the biofilm can lead to the selection of resistant strains and has a consequence evident by the occurrence of relapse of infection in animals. This study aimed to evaluate morphological and densitometric indicators of biofilm formation as well as adhesive properties of Klebsiella pneumoniae. Materials and Methods: K. pneumoniae was cultured at 37°C for 2-144 h in vitro. The specimens for optical microscopy were prepared by fixation with a 1:1 alcohol-ether mixture for 10 min and stained with a 0.5% solution of gentian violet for 2 min, and the optical density index was evaluated at a wavelength of 490 nm. Further, the adhesive properties of the microorganisms were determined at a concentration of 1 billion/ml and a suspension of ram erythrocytes at a concentration of 100 million/ml when cultured at 37°C for 24 h. Blood smears were prepared and stained with 0.5% gentian violet. Results: K. pneumonia cultured at 37°C after 24 h on the meat peptone agar formed large, convex, mucous, and white colonies (d=3.0-6.0 mm). With the growth in the meat and peptone broth, uniform turbidity of the medium was observed. Analyzing the optical density indices (density, D), it was found that K. pneumoniae were good producers of biofilms (D=0.528±0.31). Data for indicators of adhesive properties of K. pneumoniae were as follows: Average adhesion index, 4.56±0.14; adhesion coefficient, 1.07±0.52; and adhesion index, 4.26±0.07. The studied bacteria had high adhesive activity. A direct correlation dependence (R=0.94) of the optical density of biofilms (D≥0.514-0.551) and AAI (4.15±0.28-4.76±0.75) was established. Conclusion: This study has demonstrated that K. pneumoniae had high adhesive activity, was strong producer of biofilms, and the optical density of the sample exceeded the optical density of the control by more than 4 times.

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Candida Biofilm Modeling: Past and Present

Cited by 12 publications

References 0 publications

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Morphological characteristics of Candida albicans, Candida krusei, Candida guilliermondii, and Candida glabrata biofilms, and response to farnesol

Reduction in Pathogenicity in Yeast-like Fungi by Farnesol in Quail Model

Morphological and adhesive properties of Klebsiella pneumoniae biofilms

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