Candida duobushaemulonii: an emerging rare pathogenic yeast isolated from recurrent vulvovaginal candidiasis in Brazil

Boatto, Humberto Fabio; Cavalcanti, Sarah Desirée Barbosa; Negro, G. Del; Girão, Manoel Joâo Batista Castello; Francisco, Elaine Cristina; Ishida, Kelly; Gompertz, Olga Fischman

doi:10.1590/0074-02760160166

Cited by 22 publications

(17 citation statements)

References 22 publications

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“…amphotericin B [54]. Other Brazilian studies reported C. haemulonii complex isolates with low susceptibility to fluconazole or amphotericin B [58,59]. These data suggest that although these species are rare, they could be clinically relevant, and their correct identification is recommended.…”

Section: Discussionmentioning

confidence: 74%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

Preprint

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Candida spp. are the most common cause of fungal infections worldwide. The taxonomy of Candida is controversial and has undergone recent changes due to novel genetically related species. Therefore, some complexes of cryptic species have been proposed. In clinical settings, the correct identification of Candida species is relevant since some species are associated with high resistance to antifungal drugs and increased virulence. This study aimed to identify the species of four Candida complexes (C. albicans, C. glabrata, C. parapsilosis, and C. haemulonii) by molecular methods. This is the first report of six cryptic Candida species in Honduras: C. dubliniensis, C. africana, C. duobushaemulonii, C. orthopsilosis, and C. metapsilosis, and it is also the first report of the allele hwp1-2 of C. albicans sensu stricto. It was not possible to demonstrate the existence of C. auris among the isolates of the C. haemulonii complex. We also propose a simple method based on PCR-RFLP for the discrimination of the multi-resistant pathogen C. auris within the C. haemulonii complex.

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Section: Discussionmentioning

confidence: 74%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

Preprint

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“…vulnera form a fungal complex (named C. haemulonii complex) that is represented by emergent, opportunistic yeasts able to cause human infections with a wide range of clinical manifestations, varying from superficial to deep-seated infections, especially in individuals with immunocompromising health conditions [1]. In this sense, the main isolation sites of the C. haemulonii species complex described in the literature are blood, foot ulcers, nails, bones, skin wounds, and vagina; however, there are reports of isolates obtained from other body fluids such as cerebrospinal fluid, bronchoalveolar lavage, vaginal discharge, pleural effusion, peritoneal and ascitic fluids, bile, and urine [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formed by Candida haemulonii Species Complex: Structural Analysis and Extracellular Matrix Composition

Ramos

Mello

Branquinha

et al. 2020

JoF

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Candida haemulonii species complex (C. haemulonii, C. duobushaemulonii, and C. haemulonii var. vulnera) has emerged as opportunistic, multidrug-resistant yeasts able to cause fungemia. Previously, we showed that C. haemulonii complex formed biofilm on polystyrene. Biofilm is a well-known virulence attribute of Candida spp. directly associated with drug resistance. In the present study, the architecture and the main extracellular matrix (ECM) components forming the biofilm over polystyrene were investigated in clinical isolates of the C. haemulonii complex. We also evaluated the ability of these fungi to form biofilm on catheters used in medical arena. The results revealed that all fungi formed biofilms on polystyrene after 48 h at 37 °C. Microscopic analyses demonstrated a dense network of yeasts forming the biofilm structure, with water channels and ECM. Regarding ECM, proteins and carbohydrates were the main components, followed by nucleic acids and sterols. Mature biofilms were also detected on late bladder (siliconized latex), nasoenteric (polyurethane), and nasogastric (polyvinyl chloride) catheters, with the biomasses being significantly greater than on polystyrene. Collectively, our results demonstrated the ability of the C. haemulonii species complex to form biofilm on different types of inert surfaces, which is an incontestable virulence attribute associated with devices-related candidemia in hospitalized individuals.

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“…Differential diagnosis of vulvovaginal candidiasis from bacterial vaginitis and trichomoniasis is difficult based on signs and symptoms and leads to suboptimal care (7). tion by non-albicans species, such as C. glabrata, has increased (5,8). In addition, the correct identification and differentiation of etiological agents is very important for early treatment and preventing of invasion (9).…”

Section: Introductionmentioning

confidence: 99%

Identification of Candida Species Isolated from Vulvovaginal Candidiasis Patients by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Yasuj Southwestern Iran

Gharaghani

Ahmadi

Sisakht

et al. 2018

Jundishapur J Microbiol

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Background: Vulvovaginal candidiasis (VVC) is a frequent infection in females at the reproductive age. Furthermore, the most common causative agent is Candida albicans yet in the recent years, the incidence of non-albicans species has increased. Objectives: The main aim of this study was the isolation and identification of various Candida species from patients with vulvovaginal candidiasis by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Yasuj, Iran. Methods: Three hundred and ten suspected women females vaginitis were sampled and examined. Genomic DNA was extracted from fresh colonies by phenol-chloroform glass bead methods. Polymerase chain reaction (PCR) amplification was performed based on the ribosomal DNA internal transcribed spacer (rDNA-ITS), and specific electrophoretic patterns of PCR products after digestion with MspI enzyme used for species identification. Results: The cultures were positive for 160 (51.6%) vaginal samples. Candida albicans (86.8%) was the most common species among the isolates, followed by C. glabrata (3.77%) and C. krusei (3%). Multispecies with two Candida were identified in nine patients. Conclusions: Vulvovaginal candidiasis is more prevalent among females in Yasuj and the predominant agent is C. albicans. In addition, correct identification of Candida species could play an important role in management and treatment of VVC.

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Candida duobushaemulonii: an emerging rare pathogenic yeast isolated from recurrent vulvovaginal candidiasis in Brazil

Cited by 22 publications

References 22 publications

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Biofilm Formed by Candida haemulonii Species Complex: Structural Analysis and Extracellular Matrix Composition

Identification of Candida Species Isolated from Vulvovaginal Candidiasis Patients by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Yasuj Southwestern Iran

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