Candida glabrata is a successful pathogen: An artist manipulating the immune response

Castrejón-Jiménez, Nayeli Shantal; Castillo-Cruz, Juan; Baltierra‐Uribe, Shantal Lizbeth; Hernández-González, Juan Carlos; Estela, García-Pérez Blanca

doi:10.1016/j.micres.2022.127038

Cited by 12 publications

(13 citation statements)

References 209 publications

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“…C. glabrata, used for industrial pyruvate production, is also quite efficient at producing pyruvate; however, it is pathogenic in nature . This is the first report of pyruvate being produced using a natural and safe food microorganism.…”

Section: Resultsmentioning

confidence: 95%

“…9 C. glabrata, used for industrial pyruvate production, is also quite efficient at producing pyruvate; 7 however, it is pathogenic in nature. 8 This is the first report of pyruvate being produced using a natural and safe food microorganism. What is unique with this solution is that pyruvate can be generated directly in the food, where it can serve its purpose, e.g., serve as an antioxidant or be converted into the butter aroma compound diacetyl, as we explore below.…”

Section: Pyruvate Production By L Lactis Rd1m5 In Defined Medium With...mentioning

confidence: 97%

“…7 This yeast, however, is an opportunistic pathogen and not compatible with food fermentations. 8 So, there is an apparent need for food-grade microorganisms that can produce pyruvate. Previously, we used Lactococcus lactis to produce pyruvate and obtained 40.1 g/L of pyruvate using residual whey permeate as substrate.…”

Section: ■ Introductionmentioning

confidence: 99%

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Thiamine-Starved Lactococcus lactis for Producing Food-Grade Pyruvate

Zhao,

Solem

2024

J. Agric. Food Chem.

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Lactococcus lactis is a safe lactic acid bacterium widely used in dairy fermentations. Normally, its main fermentation product is lactic acid; however, L. lactis can be persuaded into producing other compounds, e.g., through genetic engineering. Here, we have explored the possibility of rewiring the metabolism of L. lactis into producing pyruvate without using genetic tools. Depriving the thiamine-auxotrophic and lactate dehydrogenase-deficient L. lactis strain RD1M5 of thiamine efficiently shut down two enzymes at the pyruvate branch, the thiamine pyrophosphate (TPP) dependent pyruvate dehydrogenase (PDHc) and αacetolactate synthase (ALS). After eliminating the remaining enzyme acting on pyruvate, the highly oxygen-sensitive pyruvate formate lyase (PFL), by simple aeration, the outcome was pyruvate production. Pyruvate could be generated by nongrowing cells and cells growing in a substrate low in thiamine, e.g., Florisil-treated milk. Pyruvate is a precursor for the butter aroma compound diacetyl. Using an α-acetolactate decarboxylase deficient L. lactis strain, pyruvate could be converted to α-acetolactate and diacetyl. Summing up, by starving L. lactis for thiamine, secretion of pyruvate could be attained. The food-grade pyruvate produced has many applications, e.g., as an antioxidant or be used to make butter aroma.

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Section: Resultsmentioning

confidence: 95%

Section: Pyruvate Production By L Lactis Rd1m5 In Defined Medium With...mentioning

confidence: 97%

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Thiamine-Starved Lactococcus lactis for Producing Food-Grade Pyruvate

Zhao,

Solem

2024

J. Agric. Food Chem.

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“…During biolm development in C. glabrata, the expression of stress response proteins gets upregulated while that of glycolytic proteins becomes downregulated, and imparts higher antifungal resistance. 10,11 Inherently, C. glabrata is less susceptible to azole drugs, and with time they are becoming resistant to other antifungals classes such as echinocandins and polyenes. 1 The prime resistance mechanisms behind antifungal therapy failure include an excessive expression of efflux pumps, alteration in drug targets, stress responses, and mutations at the genomic level.…”

Section: Introductionmentioning

confidence: 99%

“…During biofilm development in C. glabrata , the expression of stress response proteins gets upregulated while that of glycolytic proteins becomes downregulated, and imparts higher antifungal resistance. 10,11…”

Section: Introductionmentioning

confidence: 99%

Molecular investigations on Candida glabrata clinical isolates for pharmacological targeting

Gupta

Kairamkonda

et al. 2022

RSC Adv.

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Antibiofilm Activity of PDMS/TiO₂ against Candida glabrata through Inhibited Hydrophobic Recovery

Nguyen,

Sao,

Kyllo

et al. 2024

ACS Omega

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Coatings with antibiofilm properties are desirable for biomedical applications. Titanium dioxide (TiO2) has been explored as an antimicrobial agent in materials development primarily due to it being an excellent photocatalyst. Candida glabrata (C. glabrata) is an emerging human fungal pathogen with known high resistance to oxidative stress. Here, we fabricated a polydimethylsiloxane/titanium dioxide (PDMS/TiO2) nanocomposite coating and tested its antibiofilm activities against C. glabrata. The resulting nanocomposite exhibited >50% reduction in C. glabrata biofilm formation with 2.5 wt % TiO2 loading, even in the dark. Through ROS detection and surface characterization, the antibiofilm activity was attributed to the synergistic interaction of TiO2 nanoparticles with the PDMS matrix, which resulted in the impediment of hydrophobic recovery. This work provides a design strategy to develop antibiofilm coatings against C. glabrata.

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Candida glabrata is a successful pathogen: An artist manipulating the immune response

Cited by 12 publications

References 209 publications

Thiamine-Starved Lactococcus lactis for Producing Food-Grade Pyruvate

Thiamine-Starved Lactococcus lactis for Producing Food-Grade Pyruvate

Molecular investigations on Candida glabrata clinical isolates for pharmacological targeting

Antibiofilm Activity of PDMS/TiO₂ against Candida glabrata through Inhibited Hydrophobic Recovery

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Candida glabrata is a successful pathogen: An artist manipulating the immune response

Cited by 12 publications

References 209 publications

Thiamine-Starved Lactococcus lactis for Producing Food-Grade Pyruvate

Thiamine-Starved Lactococcus lactis for Producing Food-Grade Pyruvate

Molecular investigations on Candida glabrata clinical isolates for pharmacological targeting

Antibiofilm Activity of PDMS/TiO2 against Candida glabrata through Inhibited Hydrophobic Recovery

Contact Info

Product

Resources

About

Antibiofilm Activity of PDMS/TiO₂ against Candida glabrata through Inhibited Hydrophobic Recovery