Candida species biofilm and Candida albicans ALS3 polymorphisms in clinical isolates

Bruder‐Nascimento, Ariane; Camargo, Carlos Henrique; Mondelli, Alessandro Lia; Sugizaki, Mário Mateus; Sadatsune, Teruê; Bagagli, Eduardo

doi:10.1590/s1517-83822014000400030

Cited by 25 publications

(20 citation statements)

References 56 publications

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“…Muni et al [15] reported biofilm production in 64% of the 50 Candida isolates by Tube method. According to a study by Nascimento et al [16] 60.6% (36.4% high and 63.6% weak) were biofilm producers amongst 327 Candida isolates. Among them 43.1% of C. albicans and 75.8% of non-albicans Candida isolates were found to be positive for biofilm production.…”

Section: Discussionmentioning

confidence: 94%

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Tiwari¹,

Mishra²,

Dogra³

et al. 2019

SJPM

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Introduction: Candida biofilms adversely impact the health of the patients with increasing frequency and severity of disease and with soaring economic sequel. Objective: Qualitative detection of biofilm production and its quantification was performed in Candida isolates from patients infected with health care associated infection (HCAI). Method: A total of 55 Candida isolates were included in the study. Biofilm production was estimated by Tube method (TM) and Tissue culture plate method (TCP). Further quantification of the biofilm produced was performed by XTT (2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) reduction Assay and Dry weight measurement method. Result: All Candida isolates were found to be biofilm producers by all three (TM, TCP and XTT) methods. Quantity of biofilm produced by C. albicans ranged between 2.3 to 9.1 mg/disk. Among non-albicans Candida Candida tropicalis) it was between 2.2 to 7.3 mg/disk whereas non-albicans Candida (except C. tropicalis) weight of the biofilm was 2.0 to 7.1 mg/disk. Conclusion: Dry weight (DW) is the actual quantity of biofilm produced. Candida albicans produced higher quantity of biofilm than non-albicans Candida in the study. It is also concluded that quantitative detection of biofilm is definitely help clinician in deciding modality of treatment.

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Section: Discussionmentioning

confidence: 94%

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Tiwari¹,

Mishra²,

Dogra³

et al. 2019

SJPM

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show abstract

“…On the other hand HWP1 expression was not detected, It could indicate that this gene is transcribed at early stages of biofilm formation [61,62]. Overexpression of ALS3 in C. albicans was observed in initial stages of biofilm formation, that maximal expression is associated with formation of germ tubes and hyphae forms [50]. Other reports suggest that ALS3 gene was expressed in hyphae and pseudohyphae phase of C. albicans but not in yeast forms.…”

Section: Fluorescent In Situ Identification Of Als3 and Eap1 Gene Expmentioning

confidence: 94%

“…Biofilm formation occurred most frequently in isolates of C. tropicalis, followed by C. parapsilosis, C. glabrata, and C. albicans [49]. Candida non-albicans species were more prolific biofilm producers than C. albicans and that the source of isolates might influence the biofilm production [50].…”

Section: Biofilm Forming Capacitymentioning

confidence: 99%

Detection of ALS3 and EAP1 gene expresssion in Candida albicans and Candida maltosa biofilms by FISH

Calle

Cárdenas

Alvarez

2016

JBT

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I S S N 2348-6201Vol u m e 6 N u m b e r 2 J o u r n a l o f A d v a n c e s i n B i o t e c h n o l o g y 8 4 8 | P a g e S e p t e m b e r 2 0 1 6 w w w . c i r w o r l d . c o m mariateresa.alvarezaliaga@gmail.com ABSTRACTBiofilm is regarded as universal forms of microorganism life in aquatic and industrial wastewater systems as well as in a large number of environments and medical devices relevant for public health, where the exact mechanisms by which biofilm-associated microorganisms elicit infection diseases are still poorly understood.Candida biofilm formation is regulated by different mechanisms where adhesins play a clue role in the yeast attachment to certain surfaces. These adhesins are encoding by ALS3, HWP1 and EAP1 genes among others and they are also considered as Candida virulence factors.Methodologies use to study biofilm productions are intended to verify the biofilm composition, formation steps, tridimensional structure and might facilitate the monitoring of biofilm regarding, antibiotic resistance, degradations, inhibitors, enhanciement biofilm formation and other features.Here, FISH expression a modified method to detect gene expression in situ was used in order to detect ALS3, HWP1 and EAP1 in C. albicans and C. maltosa biofilms, constituting a useful tool to monitor biofilm formations. In this regard, ALS3 expression was identified in C. albicans and C. maltosa biofilms.

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“…Biofilm biomass was indirectly measured using the crystal violet (CV) staining method (70). Following incubation, biofilms were washed three times with sterile PBS to remove nonadherent cells.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Antibiofilm Activity of Eucarobustol E against Candida albicans

Liu

Shang

et al. 2017

Antimicrob Agents Chemother

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Formyl-phloroglucinol meroterpenoids (FPMs) are important types of natural products with various bioactivities. Our antifungal susceptibility assay showed that one of the Eucalyptus robusta-derived FPMs, eucarobustol E (EE), exerted a strong inhibitory effect against Candida albicans biofilms at a concentration of 16 g/ml. EE was found to block the yeast-to-hypha transition and reduce the cellular surface hydrophobicity of the biofilm cells. RNA sequencing and real-time reverse transcription-PCR analysis showed that exposure to 16 g/ml of EE resulted in marked reductions in the levels of expressions of genes involved in hyphal growth (EFG1, CPH1, TEC1, EED1, UME6, and HGC1) and cell surface protein genes (ALS3, HWP1, and SAP5). Interestingly, in response to EE, genes involved in ergosterol biosynthesis were downregulated, while the farnesol-encoding gene (DPP3) was upregulated, and these findings were in agreement with those from the quantification of ergosterol and farnesol. Combined with the obvious elevation of negative regulator genes (TUP1, NRG1), we speculated that EE's inhibition of carbon flow to ergosterol triggered the mechanisms of the negative regulation of hyphal growth and eventually led to biofilm inhibition.

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Candida species biofilm and Candida albicans ALS3 polymorphisms in clinical isolates

Cited by 25 publications

References 56 publications

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Detection of Biofilm Production and its Quantification in Candida Isolates in a Tertiary Care Hospital

Detection of ALS3 and EAP1 gene expresssion in Candida albicans and Candida maltosa biofilms by FISH

In Vitro Antibiofilm Activity of Eucarobustol E against Candida albicans

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