Candida spp./Bacteria Mixed Biofilms

Rodrigues, Maria Elisa; Gomes, Fernanda; Rodrigues, Célia Fortuna

doi:10.3390/jof6010005

Cited by 93 publications

(66 citation statements)

References 171 publications

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“…By virtue of their characteristics, the cells within these biofilms are protected against host immune mechanisms and also display high levels of resistance against most antibiotics [ 27 , 28 ]. Mixed fungal/bacterial biofilm infections are particularly hard to treat [ 10 , 29 ]. Together, C. albicans and S. aureus are responsible for a majority of opportunistic nosocomial infections [ 9 , 15 ], and they are often co-isolated from a host [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…Prior to each experiment, plates were incubated for 16–24 h at 37 °C, then a loopful of each stock culture was inoculated into 10 mL of Tryptic Soy Broth (TSB) liquid media at 37 °C for 24 h. The bacterial culture was sedimented by centrifugation (3600× g for 10 min at 4 °C), washed and resuspended in PBS and used for counting. The bacterial count of the inoculum was determined and resuspended to the final concentration (1 × 10 7 CFU/mL) on BHI broth supplemented with 10% human serum on 96 well plates and incubated at 37 °C for 18 h. TSB and BHI have been previously determined to be optimal media for supporting both C. albicans / Staphylococcus aureus (dual species) biofilm [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

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Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Lara

Lopez-Ribot

2020

Pathogens

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Both bacterial and fungal organisms display the ability to form biofilms; however, mixed bacterial/fungal biofilms are particularly difficult to control and eradicate. The opportunistic microbial pathogens Candida albicans and Staphylococcus aureus are among the most frequent causative agents of healthcare-acquired infections, and are often co-isolated forming mixed biofilms, especially from contaminated catheters. These mixed species biofilms display a high level of antibiotic resistance; thus, these infections are challenging to treat resulting in excess morbidity and mortality. In the absence of effective conventional antibiotic treatments, nanotechnology-based approaches represent a promising alternative for the treatment of highly recalcitrant polymicrobial biofilm infections. Our group has previously reported on the activity of pure positively charged silver nanoparticles synthesized by a novel microwave technique against single-species biofilms of C. albicans and S. aureus. Here, we have expanded our observations to demonstrate that that silver nanoparticles display dose-dependent activity against dual-species C. albicans/S. aureus biofilms. Moreover, the same nanoparticles were used to functionalize catheter materials, leading to the effective inhibition of the mixed fungal/bacterial biofilms. Overall, our results indicate the potent activity of silver nanoparticles against these cross-kingdom biofilms. More studies are warranted to examine the ability of functionalized catheters in the prevention of catheter-related bloodstream infections.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Lara

Lopez-Ribot

2020

Pathogens

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“…Then in 1980, Singer and Blom developed a VP made of medical-grade silicone [ 5 ]. The indwelling low-resistance Provox voice prosthesis, developed in the Netherlands Cancer Institute in 1988, and the Blom–Singer VP are the most commonly used ones nowadays [ 6 , 7 ]. They both have a similar voice quality and lifetime [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…In some cases, voice prosthesis dysfunction is not only associated with valve blockage, but also with the destruction and deformation of the entire prosthesis and fistula inflammation. Fungal biofilms on medical devices are a reservoir of pathogens that can potentially lead to local or systemic infections [ 7 , 23 ]. However, in the context of VP, it is worth noting that except for local inflammatory reactions of tracheoesophageal fistula, there is no evidence to date of serious life-threatening infection caused directly by colonized microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

et al. 2020

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Voice prosthesis implantation with the creation of a tracheoesophageal fistula is the gold standard procedure for voice rehabilitation in patients after a total laryngectomy. All patients implanted with a voice prosthesis (VP) have biofilms of fungi and bacteria grow on their surface. Biofilm colonization is one of the main reasons for VP degradation that can lead to VP dysfunction, which increases the high risk of pneumonia. In a 20-month evaluation period, 129 cases of prostheses after replacement procedures were investigated. Microbiological examination of the biofilms revealed that there were four of the most common fungi species (Candida spp.) and a large variety of bacterial species present. We studied the relationship between the time of proper function of Provox VP, the microorganism composition of the biofilm present on it, and the degradation level of the silicone material. Evaluation of the surface of the removed VP using an atomic force microscope (AFM) has demonstrated that biofilm growth might drastically change the silicone’s mechanical properties. Changes in silicone stiffness and thermal properties might contribute to the failure of VP function. Our data can serve in future studies for the development of methods to prevent or inhibit biofilm formation on the VP surface that would translate to an increase in their durability and safety.

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“…Biofilm formation involves several steps, namely the attachment to biotic or abiotic surfaces, maturation, and dispersal of mature biofilm and has long been recognized serve as a reservoir for pathogenic cells. Thus, biofilms can cause septicemia, and evolve into invasive systemic infections of organs and tissues [11]. The multifactorial nature of biofilms caused by mixed fungal-bacterial infections, along with the rapid emergence of antimicrobial resistance in pathogenic fungi and bacteria, imposes great challenges for the use of conventional antimicrobials, and further limits therapeutic options [12].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Efficacy of Chelerythrine against Mono- and Dual-Species Biofilms of Candida albicans and Staphylococcus aureus and Its Properties of Inducing Hypha-to-Yeast Transition of C. albicans

Qian

Zhang

Wang

et al. 2020

JoF

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Candida albicans and Staphylococcus aureus specifically often resulted in biofilm-associated diseases, ranging from superficial mucosal to life-threatening systemic infections. Recent studies reported that chelerythrine displayed antimicrobial activities against a few microorganisms, but its effects on mono- and dual-species biofilms of C. albicans and S. aureus have never been reported. The purpose of this study was to evaluate the efficacy of chelerythrine against mono- and dual-species biofilms, and explore its effect on the hyphal growth and the hypha-to-yeast transition of C. albicans. The results showed that minimum inhibitory concentrations (MICs) and minimum biofilm inhibitory concentration (MBIC90S) of chelerythrine against planktonic cells of mono-species were 4 and 2 μg/mL, while the MIC and MBIC90 were 6 and 3 μg/mL for dual-species. Meanwhile, the decrease in three matrix component levels and tolerance to antibiotics of biofilms formed by mono- and dual-species exposed to chelerythrine were confirmed by a confocal laser scanning microscope, in conjugation with five fluorescent dyes and a gatifloxacin diffusion assay. Moreover, C. albicans and S. aureus mono-species showed a 96.4, and 92.3% reduction, respectively, in 24-h preformed biofilm biomass in the presence of 128 µg/mL of chelerythrine. Similarly, preformed (24 h) dual-species biofilm biomass also displayed a significant reduction (90.7%) when treated with 192 μg/mL chelerythrine. Chelerythrine inhibited hyphae formation of C. albicans at 4 μg/mL, and C. albicans in hypha-form can be converted into yeast-form at 8 μg/mL of chelerythrine. Therefore, chelerythrine shows promise as a potential antimicrobial and antibiofilm agent for clinical effective treatments of mono- and mixed-species and/or biofilm-associated infections.

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Candida spp./Bacteria Mixed Biofilms

Cited by 93 publications

References 171 publications

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Inhibition of Mixed Biofilms of Candida albicans and Methicillin-Resistant Staphylococcus aureus by Positively Charged Silver Nanoparticles and Functionalized Silicone Elastomers

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

RETRACTED: Efficacy of Chelerythrine against Mono- and Dual-Species Biofilms of Candida albicans and Staphylococcus aureus and Its Properties of Inducing Hypha-to-Yeast Transition of C. albicans

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