Candida zeylanoides as a new yeast model for lipid metabolism studies: effect of nitrogen sources on fatty acid accumulation

Abstract

Lipid homeostasis is well-known in oleaginous yeasts, but there are few non-oleaginous yeast models apart from Saccharomyces cerevisiae. We are proposing the non-oleaginous yeast Candida zeylanoides QU 33 as model. The aim of this study was to investigate the influence of the carbon/nitrogen ratio and the type of nitrogen source upon oil accumulation by this yeast grown on shake flask cultures. The maximum biomass was obtained in yeast extract (2.39 ± 0.19 g/l), followed by peptone (2.24 ± 0.05 g/l), while the highest content of microbial oil (0.35 ± 0.01 g/l) and the maximum lipid yield (15.63%) were achieved with peptone. Oleic acid was the predominant cellular fatty acid in all culture media (>32.23%), followed by linoleic (>15.79%) and palmitic acids (>13.47%). The highest lipid yield using glucose and peptone was obtained at the C/N ratio of 200:1.

New yeast-based approaches in production of palmitoleic acid

“…In this context, we investigated the effect of the N source. Cell growth and accumulation of lipids were also monitored in cultivations with acetic acid and propionic acid as sole C sources or in combination with glucose, since these conditions can affect the synthesis of specific lipids (da Rosa et al, 2014). Out of the more than 600 known yeast species only about 5% are able to accumulate amounts of lipids greater than 5% of dry matter.…”

“…The FA is part of microbial lipids and can therefore be obtained in larger quantities by modifying the culture conditions that induce an increase in microbial lipid production (da Rosa et al, 2014) or synthesis of specific lipids (Papanikolaou and Aggelis, 2010). Lipid content can be influenced by carbon source (hydrophobic or hydrophilic substrates), limitations by certain elements (usually N or P), culture temperature, pH of the medium, the inoculum size, length and type of cultivation (Braunwald et al, 2013;da Rosa et al, 2014;Chen et al, 2013). To our knowledge there are currently no available studies that focus on the possibility of using yeast for the production of palmitoleic acid which, as stated above, has broad pharmaceutical and cosmetic applications.…”

The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization

“…Vieira et al ( 2014 ) assessed wild yeasts to produce microbial lipids using Brazilian molasses as raw material to accumulate fatty acids. In our group, we previously analyzed yeasts isolated from artisanal cheese as lipid producers for biodiesel or feed supplement (Poli et al, 2013 , 2014a ; Mattanna et al, 2014a , b ), or as model for lipid metabolism studies (Rosa et al, 2014 , 2015 ). In the present study, we assessed a Brazilian collection of yeasts isolated from the phylloplane of bromeliads and macrophytes, and selected the oleaginous yeast M. guilliermondii BI281A.…”

Production of Palmitoleic and Linoleic Acid in Oleaginous and Nonoleaginous Yeast Biomass

“…, R. glutinis , and S. cerevisiae . It was also found to ensure the highest lipid yield in Lipomyces starkeyi [ 42 ] whereas da Rosa et al [ 56 ] mentioned ammonium sulfate as the least appropriate nitrogen source for lipid yield in C. zeylanoides .…”