Candidate genes coding for odorant binding proteins and chemosensory proteins identified from dissected antennae and mouthparts of the southern green stink bug Nezara viridula

Wu, Zhongzhen; Yang, Chen; Qu, Meng-Qiu; Lin, Jia-Hao; Chen, Ming‐Shun; Bin, Shuying; Lin, Jintian

doi:10.1016/j.cbd.2019.100594

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…In H. halys antennal transcriptome, 17 CSPs were identified and half of them had diverse expression patterns ( Sun et al, 2020 ). In southern green stink bug Nezara viridula , 13 CSP genes were identified from antennae and mouthpart transcriptome, and only four genes were primarily expressed in antennae ( Wu et al, 2019 ). The broad and diverse expression patterns of stink bug CSPs are consistent with their possible multiple roles in chemoreception, development, and other processes.…”

Section: Discussionmentioning

confidence: 99%

“…HhalCSP15 is orthologous with NvirCSP4 (GenBank: QCZ25118.1, 81% identity) and both of them are specifically expressed in antennae, which suggests their conserved roles in olfactory perception. NvirCSP4 was expressed roughly equally in both male and female antennae ( Wu et al, 2019 ). Our data also reveal that no obvious difference was observed in expression levels of HhalCSP15 between male and female antennae.…”

Section: Discussionmentioning

confidence: 99%

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Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

Wang¹,

Yang²,

Sun³

et al. 2021

Front. Physiol.

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Chemosensory proteins (CSPs) have been identified in the sensory tissues of various insect species and are believed to be involved in chemical communication in insects. However, the physiological roles of CSPs in Halyomorpha halys, a highly invasive insect species, are rarely reported. Here, we focused on one of the antennal CSPs (HhalCSP15) and determined whether it was involved in olfactory perception. Reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) analysis showed that HhalCSP15 was enriched in nymph and male and female adult antennae, indicating its possible involvement in the chemosensory process. Fluorescence competitive binding assays revealed that three of 43 natural compounds showed binding abilities with HhalCSP15, including β-ionone (Ki=11.9±0.6μM), cis-3-hexen-1-yl benzoate (Ki=10.5±0.4μM), and methyl (2E,4E,6Z)-decatrienoate (EEZ-MDT; Ki=9.6±0.8μM). Docking analysis supported the experimental affinity for the three ligands. Additionally, the electrophysiological activities of the three ligands were further confirmed using electroantennography (EAG). EEZ-MDT is particularly interesting, as it serves as a kairomone when H. halys forages for host plants. We therefore conclude that HhalCSP15 might be involved in the detection of host-related volatiles. Our data provide a basis for further investigation of the physiological roles of CSPs in H. halys, and extend the olfactory function of CSPs in stink bugs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

Wang¹,

Yang²,

Sun³

et al. 2021

Front. Physiol.

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“…The expression of CSPs in various tissues is closely related to their functions ( Gu et al 2012 , Yang et al 2014 ). Increasing studies show that CSPs highly expressed in antennae participate in olfactory recognition ( Khuhro et al 2018 , Wu et al 2019 , Zhang et al 2020 ). In our study, the result that PxylCSP11 was highly expressed in antenna of male and female adults suggested that Pxyl CSP11 may be involved in olfactory perception.…”

Section: Discussionmentioning

confidence: 99%

Expression Profiles and Binding Properties of the Chemosensory Protein PxylCSP11 from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

ShuHui

Yan

et al. 2020

Journal of Insect Science

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The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the most destructive pests to cruciferous plants worldwide. The oligophagous moth primarily utilizes its host volatiles for foraging and oviposition. Chemosensory proteins (CSPs) are soluble carrier proteins with low molecular weight, which recognize and transport various semiochemicals in insect chemoreception. At present, there is limited information on the recognition of host volatiles by CSPs of P. xylostella. Here, we investigated expression patterns and binding characteristics of PxylCSP11 in P. xylostella. The open reading frame of PxylCSP11 was 369-bp encoding 122 amino acids. PxylCSP11 possessed four conserved cysteines, which was consistent with the typical characteristic of CSPs. PxylCSP11 was highly expressed in antennae, and the expression level of PxylCSP11 in male antennae was higher than that in female antennae. Fluorescence competitive binding assays showed that PxylCSP11 had strong binding abilities to several ligands, including volatiles of cruciferous plants, and (Z)-11-hexadecenyl acetate (Z11-16:Ac), a major sex pheromone of P. xylostella. Our results suggest that PxylCSP11 may play an important role in host recognition and spouse location in P. xylostella.

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“…The phylogenetic tree of Hemiptera was built using CSP sequences from phytophagous bugs: (1) three Aphidae species: Myzus persicae (Wang et al, 2019), Aphis gossypii (Gu et al, 2013), and Acyrthosiphon pisum (see Wang et al, 2019), (2) three Miridae plant bug species: Adelphocoris suturalis (Cui et al, 2017), Apolygus lucorum (in Wang et al, 2019), and Adelphocoris lineolatus (Sun et al, 2015), (3) three Delphacidae plant hopper species Nilaparvata lugens (in Wang et al, 2019), Laodelphax striatellus (Zhou et al, 2015), and Sogatella furcifera (Zhou et al, 2015), and (4) one Pentatomidae, Nezara viridula (Wu et al, 2019). For the Triatominae family, we used T. brasiliensis (Marchant et al, 2016a), R. prolixus (Mesquita et al, 2015), and only one sequence from R. robustus (RFG3).…”

Section: Gene Phylogeniesmentioning

confidence: 99%

Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease

et al. 2021

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Two closely related species, Rhodnius prolixus and Rhodnius robustus, are the vectors of Trypanosoma cruzi, which is the causative agent of Chagas disease, but clearly exhibit clear-cut differences in their ecological behavior. R. prolixus is considered as a domiciliated species, whereas R. robustus only sporadically visits human houses in Amazonia. We performed a chemosensory gene expression study via RNA-sequencing (RNA-seq) for the two species and also included a laboratory introgressed R. robustus strain. We built an assembled transcriptome for each sample and for both sexes and compiled all in a reference transcriptome for a differential gene expression study. Because the genes specifically expressed in one condition and not expressed in another may also reflect differences in the adaptation of organisms, a comparative study of the presence/absence of transcripts was also performed for the chemosensory transcripts, namely chemosensory proteins (CSPs), odorant-binding proteins (OBPs), odorant receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs), as well as takeout (TO) transcripts because TO proteins have been proposed to be associated with chemosensory perception in both olfactory and taste systems. In this study, 12 novel TO transcripts from the R. prolixus genome were annotated. Among the 199 transcripts, out of interest, annotated in this study, 93% were conserved between R. prolixus and the sylvatic R. robustus. Moreover, 10 transcripts out of interest were specifically expressed in one sex and absent in another. Three chemosensory transcripts were found to be expressed only in the reared R. prolixus (CSP19, OBP9, and OR89) and only one in sylvatic R. robustus (OR22). A large set of transcripts were found to be differentially expressed (DE) between males and females (1,630), with a majority of them (83%) overexpressed in males. Between environmental conditions, 8,596 transcripts were DE, with most (67%) overexpressed in the sylvatic R. robustus samples, including 17 chemosensory transcripts (4 CSPs, 1 OBP, 5 ORs, 1 GR, 4 IR, and 2 TO), but 4 genes (OBP19, OR13, OR40, and OR79) were overexpressed in the reared samples.

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Candidate genes coding for odorant binding proteins and chemosensory proteins identified from dissected antennae and mouthparts of the southern green stink bug Nezara viridula

Cited by 5 publications

References 43 publications

Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

Expression Profiles and Functional Characterization of Chemosensory Protein 15 (HhalCSP15) in the Brown Marmorated Stink Bug Halyomorpha halys

Expression Profiles and Binding Properties of the Chemosensory Protein PxylCSP11 from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

Chemosensory Gene Expression for Two Closely Relative Species Rhodnius robustus and R. prolixus (Hemiptera, Reduviidade, Triatominae) Vectors of Chagas Disease

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