Candidate pathogenicity islands in the genome of ‘<i>Candidatus</i>Rickettsiella isopodorum’, an intracellular bacterium infecting terrestrial isopod crustaceans

Wang, Yadong; Chandler, Christopher H.

doi:10.7717/peerj.2806

Cited by 12 publications

(6 citation statements)

References 57 publications

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“…These proteins are xenologs to distantly related Gammaproteobacteria (i.e., Xenorhabdus nematophila and Photorhabdus luminescens ) toxins, with the “Cand. Rickettsiella isopodorum” protein encoded within a pathogenicity island that is absent from the closely related Rickettsiella grylli ( Wang and Chandler 2016 ). This exemplifies the mobile nature of these dual PD-(D/E)XK nuclease domains and their association with large modular toxins.…”

Section: Resultsmentioning

confidence: 99%

A Tangled Web: Origins of Reproductive Parasitism

Gillespie

Driscoll

Verhoeve

et al. 2018

Genome Biology and Evolution

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While typically a flea parasite and opportunistic human pathogen, the presence of Rickettsia felis (strain LSU-Lb) in the non-blood-feeding, parthenogenetically reproducing booklouse, Liposcelis bostrychophila, provides a system to ascertain factors governing not only host transitions but also obligate reproductive parasitism (RP). Analysis of plasmid pLbAR, unique to R. felis str. LSU-Lb, revealed a toxin–antitoxin module with similar features to prophage-encoded toxin–antitoxin modules utilized by parasitic Wolbachia strains to induce another form of RP, cytoplasmic incompatibility, in their arthropod hosts. Curiously, multiple deubiquitinase and nuclease domains of the large (3,841 aa) pLbAR toxin, as well the entire antitoxin, facilitated the detection of an assortment of related proteins from diverse intracellular bacteria, including other reproductive parasites. Our description of these remarkable components of the intracellular mobilome, including their presence in certain arthropod genomes, lends insight on the evolution of RP, while invigorating research on parasite-mediated biocontrol of arthropod-borne viral and bacterial pathogens.

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Section: Resultsmentioning

confidence: 99%

A Tangled Web: Origins of Reproductive Parasitism

Gillespie

Driscoll

Verhoeve

et al. 2018

Genome Biology and Evolution

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“…Hepatoplasma crinochetorum (Mollicutes) and Ca . Hepatincola porcellionum (Alphaproteobacteria), both facultative extracellular symbionts of the midgut caeca 34 , 77 – 79 , and the bacterial pathogen Rickettsiella (Gammaproteobacteria) 34 , 80 – 82 . Each of these genera was identified as differentially abundant depending on Wolbachia infection in certain host populations: Ca .…”

Section: Resultsmentioning

confidence: 99%

Feminizing Wolbachia influence microbiota composition in the terrestrial isopod Armadillidium vulgare

Dittmer¹,

Bouchon²

2018

Sci Rep

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Wolbachia are widespread heritable endosymbionts of arthropods notorious for their profound effects on host fitness as well as for providing protection against viruses and eukaryotic parasites, indicating that they can interact with other microorganisms sharing the same host environment. Using the terrestrial isopod crustacean Armadillidium vulgare, its highly diverse microbiota (>200 bacterial genera) and its three feminizing Wolbachia strains (wVulC, wVulM, wVulP) as a model system, the present study demonstrates that Wolbachia can even influence the composition of a diverse bacterial community under both laboratory and natural conditions. While host origin is the major determinant of the taxonomic composition of the microbiota in A. vulgare, Wolbachia infection affected both the presence and, more importantly, the abundance of many bacterial taxa within each host population, possibly due to competitive interactions. Moreover, different Wolbachia strains had different impacts on microbiota composition. As such, infection with wVulC affected a higher number of taxa than infection with wVulM, possibly due to intrinsic differences in virulence and titer between these two strains. In conclusion, this study shows that heritable endosymbionts such as Wolbachia can act as biotic factors shaping the microbiota of arthropods, with as yet unknown consequences on host fitness.

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“…A metagenomics-based approach can also be useful for characterizing microbiomes, including looking for Wolbachia and other symbionts (e.g. Dittmer & Bouchon, 2018 ), and can even provide whole-genome sequence information for the symbiont (e.g., Salzberg et al, 2005 ; Richardson et al, 2012 ; Saha et al, 2012 ; Campana, Robles García & Tuross, 2015 ; Derks et al, 2015 ; Wang & Chandler, 2016 ; Lindsey et al, 2016b ; Gerth & Hurst, 2017 ). While performing a high-throughput sequencing-based screen for Wolbachia involving hundreds of different species would require a huge sampling effort and could be cost-prohibitive, screening existing sequence datasets generated for other projects offers a powerful opportunity to diagnose novel infections and better characterize variation in symbionts.…”

Section: Introductionmentioning

confidence: 99%

A bioinformatics approach to identifyingWolbachiainfections in arthropods

Pascar

Chandler

2018

PeerJ

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Wolbachia is the most widespread endosymbiont, infecting >20% of arthropod species, and capable of drastically manipulating the host’s reproductive mechanisms. Conventionally, diagnosis has relied on PCR amplification; however, PCR is not always a reliable diagnostic technique due to primer specificity, strain diversity, degree of infection and/or tissue sampled. Here, we look for evidence of Wolbachia infection across a wide array of arthropod species using a bioinformatic approach to detect the Wolbachia genes ftsZ, wsp, and the groE operon in next-generation sequencing samples available through the NCBI Sequence Read Archive. For samples showing signs of infection, we attempted to assemble entire Wolbachia genomes, and in order to better understand the relationships between hosts and symbionts, phylogenies were constructed using the assembled gene sequences. Out of the 34 species with positively identified infections, eight species of arthropod had not previously been recorded to harbor Wolbachia infection. All putative infections cluster with known representative strains belonging to supergroup A or B, which are known to only infect arthropods. This study presents an efficient bioinformatic approach for post-sequencing diagnosis and analysis of Wolbachia infection in arthropods.

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Candidate pathogenicity islands in the genome of ‘CandidatusRickettsiella isopodorum’, an intracellular bacterium infecting terrestrial isopod crustaceans

Cited by 12 publications

References 57 publications

A Tangled Web: Origins of Reproductive Parasitism

A Tangled Web: Origins of Reproductive Parasitism

Feminizing Wolbachia influence microbiota composition in the terrestrial isopod Armadillidium vulgare

A bioinformatics approach to identifyingWolbachiainfections in arthropods

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