Candidate vaccine antigens and genes in Pasteurella multocida

Adler, Ben; Bulach, Dieter; Chung, Jing Y.; Doughty, Stephen W.; Hunt, Meredith Lesley; Rajakumar, Kumar; Serrano, Maria S; Zanden, Angela Van; Zhang, Yamei; Ruffolo, Carmel G

doi:10.1016/s0168-1656(99)00111-x

Cited by 58 publications

(41 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the capsular serogroup of the isolates was A, and two of the five randomly selected isolates were somatic serotype 1. These observations are consistent with previous descriptions that the A: 1 serotype is the most common serotype in birds (Adler et al, 1999;Timoney et al, 1992). This serotype was recently reported in the isolates from turkeys in the United States (Aye et al, 2001) and from layer chicken in Pakistan (Arshad et al, 2003).…”

Section: Discussionsupporting

confidence: 94%

“…Somatic serotypes 1, 3, and 4 are the most prevalent serotypes in birds (Adler et al, 1999). Also, many P. multocida strains that have multiple somatic antigens are commonly found in avian hosts (Windingstad et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

“…All somatic serotypes have been isolated from birds. Among sixteen somatic serotypes, 1, 3 and 4 are most frequently reported (Adler et al, 1999). FC occurs sporadically or enzootically in most countries (Gilsson et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An outbreak of chronic fowl cholera in broiler breeder chickens in Korea

Kim¹,

Yoon²,

Cho³

et al. 2011

Korean Journal of Veterinary Service

View full text Add to dashboard Cite

Fowl cholera is a contagious acute and chronic disease caused by Pasteurella multocida in both domesticated and wild birds. Acute fowl cholera in both chickens and wild birds has recently been documented in Korea, but the chronic form has not been reported in Korea until now. This study describes the first outbreak of chronic fowl cholera in 13-week-old Arbor Acre broiler breeder chickens submitted to the College of Veterinary Medicine, Kyungpook National University in April 2006. The clinical signs of the affected flock of 9,621 chickens were lameness caused by swollen hock joints, diarrhea, ruffled feathers, and an average weekly mortality of 1.0%. At necropsy, purulent or caseous exudates were found in the hock and wing joints, humerus, and eyes, and severe pneumonia and pericarditis were discovered. Eleven bacterial isolates obtained from the liver, joint, infraorbital sinus and sternal bursa of the submitted chickens were all identified as Pasteurella multocida based on their physiological and biochemical characteristics. Five isolates were examined for antimicrobial susceptibility against 21 different antimicrobial agents including ampicillin. All were resistant to kanamycin, neomycin, and streptomycin, and some were resistant to gentamicin. The tested isolates were all susceptible to the other 17 antimicrobial agents. All 11 isolates were capsular serogroup A based on multiplex polymerase chain reaction. In addition, two of five isolates used in the antimicrobial susceptibility test were identified as somatic serotype 1 by an agar gel diffusion precipitin test, while the others were non-typable.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

An outbreak of chronic fowl cholera in broiler breeder chickens in Korea

Kim¹,

Yoon²,

Cho³

et al. 2011

Korean Journal of Veterinary Service

View full text Add to dashboard Cite

show abstract

“…Although P. multocida antigens have been identified that have potential as vaccines (Adler et al, 1999;Confer et al, 1996), an effective P. multocida BRD vaccine is still lacking. Killed bacterins have little effect in preventing BRD (Cardella et al, 1987); however, live vaccines have improved efficacy (Cardella et al, 1987;Chengappa et al, 1989;Panciera et al, 1984).…”

Section: Mouse Virulence Assaymentioning

confidence: 99%

Alteration of DNA adenine methylase (Dam) activity in Pasteurella multocida causes increased spontaneous mutation frequency and attenuation in mice

et al. 2003

View full text Add to dashboard Cite

Pasteurella multocida is one of the primary bacterial pathogens associated with bovine respiratory disease (BRD) complex. Relatively few virulence factors of P. multocida have been characterized, and there is a need for improved vaccines for prevention of BRD. In other Gram-negative species, DNA adenine methylase (Dam) regulates the expression of virulence genes, and appropriate expression of Dam is required for virulence. In this study, the authors cloned and sequenced the P. multocida A1 dam gene and demonstrated that it is able to restore Dam function in an Escherichia coli dam mutant. When P. multocida dam was placed under the control of a constitutively expressed promoter on a plasmid, it caused an increased spontaneous mutation rate in P. multocida. In addition, the plasmid-mediated alteration of Dam production in P. multocida caused it to be highly attenuated in mice. These findings indicate that appropriate expression of Dam is required for virulence of P. multocida, which is believed to be the first report that Dam is required for virulence of a species in the Pasteurellaceae. Therefore, Dam may function as a virulence gene regulator in the Pasteurellaceae, similar to previously reported findings from other Gram-negative species.

show abstract

“…The concentrations of aluminum hydroxide gel and ISA25 were 10 mg/ml and 5% (v/v), respectively. Formalin and gentamicin were used as preservatives at 0.1% (v/v) and 75 µg/ml, respectively [2,6,10,17].…”

Section: Bacterialmentioning

confidence: 99%

Safety and Efficacy Testing of a Novel Multivalent Bovine Bacterial Respiratory Vaccine Composed of Five Bacterins and Two Immunogens

Cho

Lee

Lim

et al. 2008

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Bovine bacterial respiratory diseases have been one of the most serious problems due to their high mortality and economic loss in calves. The vaccinations of bovine bacterial respiratory vaccines have been complex because of no multivalent vaccine. In this study, novel multivalent bovine bacterial respiratory vaccine (BRV) was developed and tested for its safety and efficacy. BRV was composed of two immunogens and five bacterins. These were leukotoxoid and bacterin of Mannheimia haemolytica type A, outer membrane protein and bacterin of Pasteurella multocida type A, and bacterins of Haemophilus somnus, Mycoplasma bovis, and Arcanobacterium pyogenes. ELISA antibody titers to five bacterial antigens in vaccinated guinea pigs increased, compared with those in unvaccinated ones. BRV was safe for calves and pregnant cattle in this study. In calves challenged with M. haemolytica and P. multocida, the average daily weight gain and antibody titers of vaccinated calves increased, and respiratory symptoms (P<0.05) and treatment frequency (P<0.01) of vaccinated calves significantly decreased, compared with those of unvaccinated calves. Interestingly, the antibody titers of M. haemolytica leukotoxoid and Mycoplasma bovis were closely related with the reduction of respiratory symptoms. BRV would be an ecomonical measure for the protection against bovine bacterial respiratory diseases. KEY WORDS: Arcanobacterium pyogenes, Haemophilus somnus, Mannheimia haemolytica leukotoxoid, Mycoplasma bovis, Pasteurella multocida outer membrane protein.

show abstract

Candidate vaccine antigens and genes in Pasteurella multocida

Cited by 58 publications

References 25 publications

An outbreak of chronic fowl cholera in broiler breeder chickens in Korea

An outbreak of chronic fowl cholera in broiler breeder chickens in Korea

Alteration of DNA adenine methylase (Dam) activity in Pasteurella multocida causes increased spontaneous mutation frequency and attenuation in mice

Safety and Efficacy Testing of a Novel Multivalent Bovine Bacterial Respiratory Vaccine Composed of Five Bacterins and Two Immunogens

Contact Info

Product

Resources

About