Candidate Vaccine Focused on a Classical Swine Fever Virus Epitope Induced Antibodies with Neutralizing Activity

Qi, Y.; Zhang, Bing-Qing; Shen, Zhenjiang; Chen, Yinghua

doi:10.1089/vim.2009.0007

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…Many indirect ELISAs based on recombinant E2, E rns or NS3 are available for specific antibody detection [ 8 , 20 , 31 , 34 , 35 ]. Because E2 is the primary target for neutralizing antibody [ 9 , 36 ], the E2 -based ELISAs are widely used for CSF diagnosis and evaluation of vaccination efficiency. However, the available E2 -based ELISA tests can not distinguish CSFV from BVDV infection and usually lead to false positive [ 34 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

The recombinant Erns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

Zhu

et al. 2022

Virol J

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Background Classical swine fever (CSF) virus is the causative agent of an economically important, highly contagious disease of pigs. CSFV is genetically and serologically related to bovine viral diarrhea virus (BVDV). BVDV infection in pigs can mimic CSF clinical signs, which cause difficulty in differentiation. Serological test for detection of virus specific antibodies is a valuable tool for diagnosis and surveillance of CSFV and BVDV infections in animals. The aim of this study was to develop the CSFV Erns and BVDV tE2 -based ELISAs to distinguishably test specific antibodies against CSFV and BVDV. Methods The CSFV Erns and truncated E2 (tE2, residues 690–865) of BVDV were expressed in E. coli and purified by Ni–NTA affinity chromatography, respectively. Employing Erns or tE2 protein as diagnostic antigen, indirect ELISAs were developed to distinguishably test specific antibodies against CSFV and BVDV. The specificity and sensitivity of ELISAs were evaluated using a panel of virus specific sera of pigs, immunized rabbits and immunized mice. A total 150 clinical serum samples from farm pigs were measured by the developed ELISAs and compared with virus neutralizing test (VNT). Results Indirect ELISA was established based on recombinant CSFV Erns or BVDV tE2 protein, respectively. No serological cross-reaction between antibodies against CSFV and BVDV was observed in sera of immunized rabbits, immunized mice or farm pigs by detections of the Erns and tE2 -based ELISAs. Compared to VNT, the CSFV Erns -based ELISA displayed a high sensitivity (93.3%), specificity (92.0%) and agreement rate (92.7%), and the sensitivity, specificity and agreement rate of BVDV tE2 -based ELISA was 92.3%, 95.2% and 94.7%, respectively. Conclusion The newly developed ELISAs are highly specific and sensitive and would be valuable tools for serological diagnosis for CSFV and BVDV infections.

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Section: Discussionmentioning

confidence: 99%

The recombinant Erns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

Zhu

et al. 2022

Virol J

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“…Sự thành công này sẽ là cơ sở quan trọng để chúng tôi tiếp tục nghiên cứu tạo ra protein E2 tái tổ hợp, là nguyên liệu cho các loại vaccine tiểu đơn vị chống lại bệnh CSF. Thực tế, protein E2 chịu trách nhiệm chính trong việc kích ứng tạo ra các kháng thể trung hòa CSFV, hình thành miễn dịch bảo hộ trong quá trình nhiễm trùng [15]. Trong số các protein của CSFV, E2 là một trong hai kháng nguyên chủ yếu được dùng để sản xuất vaccine [16].…”

Section: Kết Quả Giải Trình Tự đOạn Gene E2unclassified

Tạo Dòng Và Giải Trình Tự Toàn Bộ Gene E2 Của Virus Dịch Tả Heo Cổ Điển Ở Một Số Trại Heo Tại Miền Nam Việt Nam

Mai¹,

Bình²,

Danh³

et al. 2022

TNUJST

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Bệnh dịch tả heo cổ điển (Classical swine fever disease) là một bệnh lâu đời, nguy hiểm và gây thiệt hại kinh tế đáng kể cho ngành chăn nuôi heo trên toàn thế giới. Trình tự gene mã hóa protein E2 là một cơ sở quan trọng để đánh giá, so sánh đa dạng di truyền của CSFV và phát triển vaccine. Nghiên cứu này được tiến hành nhằm tạo ra các dòng E. coli mang plasmid chứa đầy đủ trình tự gene E2. 21 mẫu dương tính với CSFV đã được thu thập được biến nạp vào các dòng E. coli DH5α. Các plasmid sau đó được tinh sạch và giải trình tự nhằm đánh giá hiệu quả của phương pháp tạo dòng. Kết quả cho thấy nghiên cứu đã tạo thành công 21 dòng vi khuẩn E. coli mang toàn bộ gene E2 của CSFV. Các trình tự này tương đồng cao (87,94 – 98,84%) khi so sánh với 100 trình tự có độ tương đồng cao nhất trên NCBI. Ngoài ra, 19 trình tự tương đồng rất cao với các chủng thuộc phân nhóm 2.1c (95,00 – 96,34%) và 2 trình tự với phân nhóm 2.2 (92,05 – 93,03%). Nghiên cứu này là cơ sở cho việc áp dụng kĩ thuật tạo dòng E. coli mang gene E2 trong việc phân tích di truyền của CSFV một cách ổn định, cũng là cơ sở cho sự tạo ra các protein E2 tái tổ hợp phục vụ việc phát triển vaccine tiểu đơn vị chống CSFV sau này.

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“…E2 contains four relatively antigenic domains: A, B, C, and D [7]. e E2 protein is the preferred candidate antigen for the development of novel vaccine and diagnostic methods for CSF [8,9]. e variable domain fragments of camelid immunoglobulin heavy-chain antibodies (HCAbs), also called nanobodies (Nbs) or single-domain antibodies (sdAbs), have been identified and characterized over the past few decades.…”

Section: Introductionmentioning

confidence: 99%

Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots

Yang¹,

Li²,

Shang³

et al. 2020

BioMed Research International

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Outbreak of classical swine fever (CSF) results in high mortality and thus causes severe economic losses in the swine industry. Single-domain antibody (sdAb) is the smallest antigen-binding molecule derived from camelid heavy-chain antibodies and has the potential to be used as a molecular probe for detection of CSF virus (CSFV). In this study, two sdAb fragments against the E2 antigen of CSFV were obtained, expressed in vitro. e functional characteristics analysis indicated that the recombinant sdAbE2-1 and sdAbE2-2 have excellent binding activity, specificity, and high affinity with equilibrium constant value of 3.34 × 10 − 7 and 1.35 × 10 − 8 M to E2 protein. en, sdAbE2s were conjugated with quantum dots (QD)/AF488 to synthesize two molecular probes for imaging CSFV distribution in cells. e sdAbE2-1 was also labeled with carboxyl-magnetic beads to construct immunomagnetic nanobeads (IMNBs) able to capture CSFV virions and recombinant E2 protein. QD/AF455-sdAbE2s probes colocalised with CSFV virions in swine testis cells, and IMNBs were used as a detection template and proved to bind specifically with CSFV virions and E2 protein. e selected sdAb fragments and sdAb-based molecular probes may be used for the rapid identification of CSFV during field outbreaks and for research on CSFV and host interactions.

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Candidate Vaccine Focused on a Classical Swine Fever Virus Epitope Induced Antibodies with Neutralizing Activity

Cited by 13 publications

References 31 publications

The recombinant Erns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

The recombinant Erns and truncated E2-based indirect enzyme-linked immunosorbent assays to distinguishably test specific antibodies against classical swine fever virus and bovine viral diarrhea virus

Tạo Dòng Và Giải Trình Tự Toàn Bộ Gene E2 Của Virus Dịch Tả Heo Cổ Điển Ở Một Số Trại Heo Tại Miền Nam Việt Nam

Selection and Characterization of CSFV-Specific Single-Domain Antibodies and Their Application along with Immunomagnetic Nanobeads and Quantum Dots

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