“Candidatus Neoehrlichia mikurensis” Infection in a Dog from Germany

Diniz, Pedro; Schulz, Bianka; Hartmann, Katrin; Breitschwerdt, Edward B.

doi:10.1128/jcm.02327-10

Cited by 68 publications

(57 citation statements)

References 16 publications

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“…"Candidatus Neoehrlichia mikurensis" can currently be considered an emerging pathogen of veterinary and medical importance. In 2007, the bacterium was identified in a dog with hematological troubles in Germany (17). In 2010, four human cases were reported in Switzerland, Germany, and Sweden (18,60,61), one of which was fatal.…”

Section: Discussionmentioning

confidence: 99%

Infections and Coinfections of Questing Ixodes ricinus Ticks by Emerging Zoonotic Pathogens in Western Switzerland

Lommano

Bertaiola

Dupasquier

et al. 2012

Appl Environ Microbiol

115

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ABSTRACTIn Europe,Ixodes ricinusis the vector of many pathogens of medical and veterinary relevance, among themBorrelia burgdorferisensu lato and tick-borne encephalitis virus, which have been the subject of numerous investigations. Less is known about the occurrence of emerging tick-borne pathogens likeRickettsiaspp.,Babesiaspp., “CandidatusNeoehrlichia mikurensis,” andAnaplasma phagocytophilumin questing ticks. In this study, questing nymph and adultI. ricinusticks were collected at 11 sites located in Western Switzerland. A total of 1,476 ticks were analyzed individually for the simultaneous presence ofB. burgdorferisensu lato,Rickettsiaspp.,Babesiaspp., “CandidatusNeoehrlichia mikurensis,” andA. phagocytophilum. B. burgdorferisensu lato,Rickettsiaspp., and “CandidatusNeoehrlichia mikurensis” were detected in ticks at all sites with global prevalences of 22.5%, 10.2%, and 6.4%, respectively.Babesia- andA. phagocytophilum-infected ticks showed a more restricted geographic distribution, and their prevalences were lower (1.9% and 1.5%, respectively). Species rarely reported in Switzerland, likeBorrelia spielmanii,Borrelia lusitaniae, andRickettsia monacensis, were identified. Infections with more than one pathogenic species, involving mostlyBorreliaspp. andRickettsia helvetica, were detected in 19.6% of infected ticks. Globally, 34.2% of ticks were infected with at least one pathogen. The diversity of tick-borne pathogens detected inI. ricinusin this study and the frequency of coinfections underline the need to take them seriously into consideration when evaluating the risks of infection following a tick bite.

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Section: Discussionmentioning

confidence: 99%

Infections and Coinfections of Questing Ixodes ricinus Ticks by Emerging Zoonotic Pathogens in Western Switzerland

Lommano

Bertaiola

Dupasquier

et al. 2012

Appl Environ Microbiol

115

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“…Dogs are frequently exposed to ticks. In 2007, 'Candidatus Neoehrlichia mikurensis' was detected in an 8-year-old female spayed Irish setter in Germany (Diniz et al, 2011). The dog had undergone bilateral mastectomy and ovariohysterectomy; subsequently, the animal became lethargic and exhibited profuse subcutaneous hemorrhages.…”

Section: Introductionmentioning

confidence: 99%

Detection of ‘Candidatus Neoehrlichia mikurensis’ and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries

Hofmann‐Lehmann

Wagmann

Meli

et al. 2016

SAT

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“…Neoehrlichia mikurensis" was found in rodent populations in China (17), Japan (18), Sweden (19), and the Netherlands (6). Moreover, an infection was described in a dog in Germany with symptoms similar to ehrlichiosis (20).…”

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confidence: 99%

Close Geographic Association of Human Neoehrlichiosis and Tick Populations Carrying “Candidatus Neoehrlichia mikurensis” in Eastern Switzerland

et al. 2013

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e Neoehrlichiosis caused by "Candidatus Neoehrlichia mikurensis" is an emerging zoonotic disease. In total, six patients have been described in Europe, with the first case detected in 2007. In addition, seven patients from China were described in a report published in October 2012. In 2009, we diagnosed the first human case of "Ca. Neoehrlichia mikurensis" infection in the Zurich area (Switzerland). Here, we report two additional human cases from the same region, which were identified by broad-range 16S rRNA gene PCR. Both patients were immunocompromised and presented with similar clinical syndromes, including fever, malaise, and weight loss. A diagnostic multiplex real-time PCR was developed for specific detection of "Ca. Neoehrlichia mikurensis" infections. The assay is based on the signature sequence of a 280-bp fragment of the "Ca. Neoehrlichia mikurensis" 16S rRNA gene and incorporates a "Ca. Neoehrlichia mikurensis" species, a "Ca. Neoehrlichia" genus, and an Anaplasmataceae family probe for simultaneous screening. The analytical sensitivity was determined to be below five copies of the "Ca. Neoehrlichia mikurensis" 16S rRNA gene. Our results show that the assay is suitable for the direct detection of "Ca. Neoehrlichia mikurensis" DNA in clinical samples from, for example, blood and bone marrow. In addition, it allows for monitoring treatment response during antibiotic therapy. Using the same assay, DNA extracts from 1,916 ticks collected in four forests in close proximity to the patients' residences (<3 km) were screened. At all sampling sites, the minimal prevalence of "Ca. Neoehrlichia mikurensis" was between 3.5 to 8% in pools of either nymphs, males, or females, showing a strong geographic association between the three patients and the assumed vector.

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“Candidatus Neoehrlichia mikurensis” Infection in a Dog from Germany

Cited by 68 publications

References 16 publications

Infections and Coinfections of Questing Ixodes ricinus Ticks by Emerging Zoonotic Pathogens in Western Switzerland

Infections and Coinfections of Questing Ixodes ricinus Ticks by Emerging Zoonotic Pathogens in Western Switzerland

Detection of ‘Candidatus Neoehrlichia mikurensis’ and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries

Close Geographic Association of Human Neoehrlichiosis and Tick Populations Carrying “Candidatus Neoehrlichia mikurensis” in Eastern Switzerland

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