‘Candidatus Phytoplasma hispanicum’, a novel taxon associated with Mexican periwinkle virescence disease of Catharanthus roseus

Davis, Robert E.; Harrison, N. A.; Zhao, Yan; Wei, Wei; Dally, E. L.

doi:10.1099/ijsem.0.001218

Cited by 41 publications

(23 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The identification of a 16SrXIII‐A phytoplasma revealed amaranth as a new host for this subgroup, contributing for extend the knowledge about the diversity of plant hosts. In addition, we report the first occurrence of 16SrXIII‐A phytoplasma in Argentina and consequently in South America, because this strain was previously identified only in Mexico (Davis et al, ).…”

Section: Discussionmentioning

confidence: 81%

“…The sequence of the ASP‐Ar1 phytoplasma shared 98.8% similarity with sequence of the reference phytoplasma of the group 16Sr XIII (Mexican periwinkle virescence phytoplasma) a ‘Ca. Phytoplasma hispanicum’‐related strain (Davis, Zhao, & Dally, ). Based on computer‐simulated virtual RFLP patterns, a value of similarity coefficient (F) equal to 0.98 was obtained, when the ASP‐Ar1 phytoplasma identified in amaranth was contrasted with the representative of the subgroup 16SrXIII‐A (Table ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Amaranthus caudatus subsp. mantegazzianus: A new host of ‘Candidatus Phytoplasma hispanicum’ (subgroup 16Sr XIII‐A)

Noelting

Ferreira

Galvão

et al. 2019

Journal of Phytopathology

View full text Add to dashboard Cite

In Argentina, amaranth is a promising crop due to high nutritional quality and ability to grow in a diversity of environments. In areas cultivated with amaranth, were observed plants exhibiting slow growth, deformed leaves, proliferation of shoots and malformed lateral panicles. Field survey revealed up to 96% disease incidence and 92% of the seeds collected from mother plants produced diseased seedlings. A phytoplasma was detected in association with seedlings and adult plants using nested PCR assays. Molecular identification by computer‐simulated RFLP and phylogenetic analysis evidenced the occurrence of a ‘Candidatus Phytoplasma hispanicum’‐related strain, affiliated with 16SrXIII‐A subgroup. The findings implicate amaranth as a new host for this subgroup and as a potential reservoir of the pathogen for other cultivated species. In addition, to the best of our knowledge, this study reports for the first time the presence of 16SrXIII‐A phytoplasma in Argentina and in South America. Furthermore, transmission assays pointed that naturally infected seed is an important vehicle of dissemination of the pathogen, threatening the expansion of the crop for new geographical areas.

show abstract

Section: Discussionmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Amaranthus caudatus subsp. mantegazzianus: A new host of ‘Candidatus Phytoplasma hispanicum’ (subgroup 16Sr XIII‐A)

Noelting

Ferreira

Galvão

et al. 2019

Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…Recently, two species of ‘ Candidatus Phytoplasma’ have been described among the members of the 16SrXIII group, ‘ Ca . Phytoplasma hispanicum’ 28 and ‘ Ca . Phytoplasma maliae’ 52 which have been reported affecting periwinkle 27 , strawberry 24, 26 , potato 29 , papaya 53 , broccoli 47 , and china tree 54 plants.…”

Section: Discussionmentioning

confidence: 99%

“…Phytoplasma’ species, ‘ Ca . Phytoplasma hispanicum’ 28 . Other plants, such as potato, have also been identified as hosts, but members of this phytoplasma group have not been described outside of the Americas 29 .…”

Section: Introductionmentioning

confidence: 99%

Molecular diagnostic assays based on cpn60 UT sequences reveal the geographic distribution of subgroup 16SrXIII-(A/I)I phytoplasma in Mexico

Pérez‐López

Rodríguez-Martínez

Olivier

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Geographically diverse samples from strawberry exhibiting symptoms of Strawberry Green Petal (SbGP), periwinkle plants with virescence, and blackberry, blueberry, and raspberry plants displaying yellowing and inedible fruits, were assayed for the presence of phytoplasma DNA. PCR targeting the 16S rRNA-encoding gene and chaperonin-60 (cpn60) showed that the plants were infected with phytoplasma subgroup16SrXIII-(A/I)I (SbGP/MPV). To examine the geographic distribution of this pathogen in Mexico, we designed an array of cpn60-targeted molecular diagnostic assays for SbGP/MPV phytoplasma. A fluorescent microsphere hybridization assay was designed that was capable of detecting SbGP/MPV phytoplasma in infected plant tissues, successfully differentiating it from other known phytoplasma cpn60 UT sequences, while identifying a double infection with SbGP/MPV and aster yellows (16SrI) phytoplasma. Two quantitative assays, quantitative real-time PCR (qRT-PCR) and droplet digital PCR (ddPCR), gave similar results in infected samples. Finally, a loop-mediated isothermal amplification (LAMP) assay provided rapid detection of SbGP/MPV phytoplasma DNA. Application of these assays revealed that SbGP/MPV phytoplasma is widely distributed in Central Mexico, with positive samples identified from eleven localities within three states separated by hundreds of kilometres. These results also provide tools for determining the presence and geographic distribution of this pathogen in plant and insect samples in other localities.

show abstract

“…musculi' and 'Candidatus Mycoplasma haemomuris subsp. ratti' [6]; 'Candidatus Phytoplasma cirsii' [7]; 'Candidatus Phytoplasma hispanicum' [8]. There was discussion regarding the standards for subspecies of 'Candidatus' mollicutes.…”

Section: Minute 10 New Species Of Mollicutesmentioning

confidence: 99%

International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Mollicutes. Minutes of the closed meeting, 3rd July 2016, Brisbane, Australia

May

Brown

2017

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

‘Candidatus Phytoplasma hispanicum’, a novel taxon associated with Mexican periwinkle virescence disease of Catharanthus roseus

Cited by 41 publications

References 27 publications

Amaranthus caudatus subsp. mantegazzianus: A new host of ‘Candidatus Phytoplasma hispanicum’ (subgroup 16Sr XIII‐A)

Amaranthus caudatus subsp. mantegazzianus: A new host of ‘Candidatus Phytoplasma hispanicum’ (subgroup 16Sr XIII‐A)

Molecular diagnostic assays based on cpn60 UT sequences reveal the geographic distribution of subgroup 16SrXIII-(A/I)I phytoplasma in Mexico

International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Mollicutes. Minutes of the closed meeting, 3rd July 2016, Brisbane, Australia

Contact Info

Product

Resources

About