Candidemia: Speciation and Antifungal susceptibility testing from a Tertiary Care Hospital in Maharashtra, India

Gandham, Nageswari; Vyawahare, Chanda; Jadhav, Savita; Misra, Rabindra Nath

doi:10.4103/0975-2870.192165

Cited by 10 publications

(15 citation statements)

References 2 publications

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“…Voriconazole has high susceptibility rates in many of the earlier studies. [14] In our study, micafungin shows 100% sensitivity to all the 78 Candida isolates tested. Resistance to flucytosine and caspofungin was seen only for C. krusei.…”

Section: Discussionsupporting

confidence: 47%

“…This finding is consistent with many similar Indian studies also. [12] A study from Mumbai in 2019 has shown highest isolation rates for C. tropicalis (10/29) followed by C. parapsilosis ( 8/29), C. albicans (3/29) and C. krusei (3/29) [14] . In a 3 years study conducted in trauma patients at All India Institute of Medical Sciences, C. tropicalis, 82 (38.7%) was the most common isolated followed by C. parapsilosis, 43 (20.3%) and C. albicans, 29 (13.7%).…”

Section: Discussionmentioning

confidence: 99%

“…[16] Candida krusei (15%), Candida parapsilosis (16.25%) and Candida tropicalis (28.75%) are the predominant species from blood stream infections in our study. Candida parapsilosis has emerged as an important agent in nosocomial infections in several studies as reported in a review by Sardi et al [17] In some other Indian studies like that of Singh et al [18] , Chakrabarti A et al [19] , Xess et al [15] , C. tropicalis is identified as the predominant species causing candidemia in North India [15] and Maharashtra [14] . In contrast to these findings C. albicans still constitute the most prevalent fungal species in candidemia (46.3%), followed by C. parapsilosis (19.5%), C. glabrata (15.9%), C. tropicalis (14.6%) in a study from China by Xiao et al [20] In the present study we got 4 isolates of C. pelliculosa also which were isolated from an outbreak of nosocomial fungaemia in neonatal intensive care unit (NNICU).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Speciation and Antifungal Susceptibility Profile of Candida Species in a Tertiary Care Centre in Central Kerala

Bhaskaran¹,

Valsan²,

A³

2020

jemds

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular Speciation and Antifungal Susceptibility Profile of Candida Species in a Tertiary Care Centre in Central Kerala

Bhaskaran¹,

Valsan²,

A³

2020

jemds

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“…Candida non-albicans have immensely replaced Candida albicans in majority of candida infections, although the distribution of species varies with the geographical region and patient population. [1][2][3] Candida tropicalis has been found to be the major fungal isolate from blood stream infection/candidemia in India and Taiwan, whereas Candida parapsilosis and Candida glabrata have been found to be prominent in China and the US respectively. 2,4 Candida has been reported as the fourth to sixth common species isolated from nosocomial infections in the USA and Switzerland.…”

Section: Introductionmentioning

confidence: 99%

“…Mortality rates among these patients are high due to candidemia (30-81% cases) and delayed specific antifungal therapy. 1,5 Approximately 3355% of all candidemia reports arise from intensive care units (ICUs). 5 The portal of entry in majority of cases is the gut, where they thrive and can break the intestinal layers to enter bloodstream, other portals include skin, in patients with indwelling catheters (central venous catheters; CVCs).…”

Section: Introductionmentioning

confidence: 99%

A Study on Prevalence and Characterization of Candida Species in Immunocompromised Patients

Nandini¹,

Manonmoney²,

Lavanya³

et al. 2021

J. Pure Appl. Microbiol.

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Candida spp. is one among the major causes of nosocomial infection, with candidemia gaining increasing prevalence worldwide in parallel with mortality rates ranging from 10-49%. Epidemiology and predisposing factors of candidemia have changed since the number of patients receiving transplants and immunosuppressive therapy, the use of broadspectrum antimicrobials, and the number of AIDS patients have increased. Candidemia is more common among patients with subcutaneous and cutaneous candida infections, through percutaneous inoculation. Major predisposing factors for invasive candidiasis includes neutropenia, haematological malignancies, bone marrow transplantation, total parenteral nutrition, chemotherapy, invasive procedures, and immune-suppressive agents. This study analyses the risk factors of immunocompromised patients with candidemia and antibiogram of Candida spp. isolated from ICU patients. To evaluate the prevalence, distribution and antibiogram of Candida spp., associated risk factors, and outcome in candidemia patients. Blood samples received from patients with clinically suspected fungal infections were subjected to gram staining, culture, sugar assimilation & fermentation, Candida Chrome agar (CCA) & Corn meal agar for identification and speciation. Antifungal susceptibility tests were performed by disk-diffusion tests. Among a total of 337 samples received, 22 (6.5%) samples were positive for candida infections, of which Candida tropicalis 9 (41%) was the predominant isolate followed by C. albicans 5 (23%), Candida glabrata 4 (18%), Candida parapsilosis 2 (9%) and Candida krusei 2 (9%). Male patients had a higher prevalence of candidemia 15 (68.2%). Among the age group of 51-70 years, uncontrolled DM(Diabetes mellitus) and CKD (chronic kidney disease) were found to be the predominant co-morbidities with candidemia.

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A retrospective investigation of Candida spp. and its antifungal susceptibility profile in cancer patients at a South Indian tertiary care hospital

Vijayakumar,

Emelda,

Radhakrishnan

et al. 2023

IJMMTD

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Among the most frequently recognized pathogenic fungi, . from Clinical samples plays a vital role. In recent decades, poor antifungal therapy usage critically in immunocompromised patients has led to antifungal drug resistance among . Hence, expeditious and dependable species identification is crucial for the effective implantation of treatment strategies and the management of diseases. The purpose of the study was to look at the presence of in various Clinical samples taken from cancer patients who are being treated at a tertiary care hospital in South India. The primary objective of this investigation is to ascertain the susceptibility patterns shown by antifungal drugs. This retrospective investigation was undertaken between January 2022 to April 2023. The isolates were subjected to antifungal susceptibility (AFST) by both Conventional (disc diffusion assay) and Automated methods (VITEK-2 compact - AST YSO1). The susceptibility pattern was recorded against antifungal agents like Fluconazole (FLU), Voriconazole (VOR), Amphotericin B (AMB), and Caspofungin. . was identified by conventional method (Chrome ID) as per standard laboratory protocols and by an automated method using MALDI – TOF. Among 215 samples received from cancer patients suggestive of Candidiasis, albicans (64.6%) was the predominant isolate, and the lowest one was (2.25%). Male patients had a higher prevalence of (55.8%). Similarly, the prevalence was higher in patients over 60, with male patients having an incidence of 38.3% and female patients having 23%. By disk diffusion assay and showed 100% sensitivity to all antifungals used in this study. We conclude that the disc diffusion assay is a more cost-effective, user-friendly, and effective screening test than the VITEK 2, and it has the potential to be used as a workable method for Antifungal susceptibility test. The use of Chrome ID for identification of . is cost-effective and reliable. However, MALDI TOF has the advantage of rapidity, providing correct findings promptly. The timely delivery of diagnostic reports facilitates early diagnosis of patients. Therefore, it is recommended in this research to use MALDI TOF for the identification of . and the disc diffusion technique for antifungal susceptibility testing (AFST).

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Candidemia: Speciation and Antifungal susceptibility testing from a Tertiary Care Hospital in Maharashtra, India

Cited by 10 publications

References 2 publications

Molecular Speciation and Antifungal Susceptibility Profile of Candida Species in a Tertiary Care Centre in Central Kerala

Molecular Speciation and Antifungal Susceptibility Profile of Candida Species in a Tertiary Care Centre in Central Kerala

A Study on Prevalence and Characterization of Candida Species in Immunocompromised Patients

A retrospective investigation of Candida spp. and its antifungal susceptibility profile in cancer patients at a South Indian tertiary care hospital

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