2016
DOI: 10.3389/fpls.2015.01171
|View full text |Cite
|
Sign up to set email alerts
|

CandiSSR: An Efficient Pipeline used for Identifying Candidate Polymorphic SSRs Based on Multiple Assembled Sequences

Abstract: Simple sequence repeats (SSRs), also known as microsatellites, are ubiquitous short tandem duplications commonly found in genomes and/or transcriptomes of diverse organisms. They represent one of the most powerful molecular markers for genetic analysis and breeding programs because of their high mutation rate and neutral evolution. However, traditionally experimental screening of the SSR polymorphic status and their subsequent applicability to genetic studies are extremely labor-intensive and time-consuming. T… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
58
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 55 publications
(59 citation statements)
references
References 48 publications
1
58
0
Order By: Relevance
“…This tool was built to help users to massively detect polymorphic SSRs (PolySSR) in tea plant, an import and widely used genetic markers for tea plant population genetic study and molecular breeding. Our previously developed CandiSSR pipeline was deployed online to achieve this goal (Xia et al ., ). Users can upload the assembled transcript sequences, or genomic sequences, or even organelle sequences to search polySSRs.…”
Section: Facilities and Toolsmentioning
confidence: 97%
See 1 more Smart Citation
“…This tool was built to help users to massively detect polymorphic SSRs (PolySSR) in tea plant, an import and widely used genetic markers for tea plant population genetic study and molecular breeding. Our previously developed CandiSSR pipeline was deployed online to achieve this goal (Xia et al ., ). Users can upload the assembled transcript sequences, or genomic sequences, or even organelle sequences to search polySSRs.…”
Section: Facilities and Toolsmentioning
confidence: 97%
“…The CandiSSR pipeline (Xia et al ., ) was used to identify PloySSRs between tea plant and other 19 representative Camellia species. In total, 1663 polymorphic EST‐SSRs were identified.…”
Section: Data Contentmentioning
confidence: 99%
“…CandiSSR was used to identify potential polymorphic genic SSRs among the six unigenes datasets of T. mongolica [39]. The parameters used in the pipeline were: flanking sequence length 100 bp, blast e-value cutoff 1.0 × e −10 , blast identity cutoff 95%, and blast coverage cutoff 95% [39,40].…”
Section: Identification Of Potential Polymorphic Genic Ssrsmentioning
confidence: 99%
“…PolyMorphPredict (Das et al, ) and PSR (Cantarella & D'Agostino, ) lack the masking of mutations in primer design. PolySSR (Tang et al, ) and CandiSSR (Xia et al, ) depend on assembled sequences. iMSAT (Andersen & Mills, ) is dependent on (insertion and deletion) Indel calling and lacks the masking of mutations in primer design.…”
Section: Introductionmentioning
confidence: 99%
“…PolySSR (Tang et al, 2008) and CandiSSR (Xia et al, 2015) depend on assembled sequences. iMSAT (Andersen & Mills, 2014) is dependent on (insertion and deletion) Indel calling and lacks the masking of mutations in primer design.…”
Section: Introductionmentioning
confidence: 99%