Canid Reproductive Biology: Norm and Unique Aspects in Strategies and Mechanisms

Nagashima, Jennifer; Songsasen, Nucharin

doi:10.3390/ani11030653

Cited by 19 publications

(4 citation statements)

References 166 publications

(215 reference statements)

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“…Knowledge gained from such cross-species comparisons highlights the diversity that exists [ 33 ]. In a commercial setting, reduced fertility rates may be because of the lack of species-specific knowledge.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of oviductal organoids from farm and companion animals

Lawson

Ghosh

Blanch

et al. 2023

Biology of Reproduction

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Organoid technology has provided a unique opportunity to study early human development and decipher various steps involved in the pathogenesis of disease. The technology is already used in clinics to improve human patient outcomes. However, limited knowledge of the methodologies required to establish organoid culture systems in domestic animals has slowed the advancement and application of organoid technology in veterinary medicine. This is particularly true for the field of reproduction and the application of assisted reproductive technologies (ART). Here, we have developed a platform to grow oviductal organoids from five domestic species – bovine, porcine, equine, feline, and canine. The organoids were grown progressively from single cells derived from the enzymatic digestion of freshly collected infundibular/fimbrial samples. The addition of WNT, TGFβ, BMP, ROCK, and Notch signalling pathway activators or inhibitors to the organoid culture medium suggested remarkable conservation of the molecular signals involved in oviductal epithelial development and differentiation across species. The gross morphology of organoids from all the domestic species was initially similar. However, some differences in size, complexity, and growth rate were subsequently observed and described. After 21 days, well-defined and synchronized motile ciliated cells were observed in organoids. Histopathologically, oviductal organoids mimicked their respective native tissue. In summary, we have carried out a detailed cross-species comparison of oviductal organoids, which would be valuable in advancing our knowledge of oviduct physiology and, potentially, help in increasing the success of assisted reproductive technologies.

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Section: Discussionmentioning

confidence: 99%

Establishment and characterization of oviductal organoids from farm and companion animals

Lawson

Ghosh

Blanch

et al. 2023

Biology of Reproduction

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“…Author details 1 Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. 2 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.…”

Section: Discussionmentioning

confidence: 99%

“…Canines can be bred so long as they are predominantly fertile, and maintaining this species's reproductive ability remains an issue of interest [ 1 ]. A male's potential fertility can be predicted by evaluating the quality of the semen before the beginning of its reproductive life [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs

et al. 2022

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This study aimed to determine the effects of melatonin administration on testicular vascular perfusion in relation to steroid hormones and semen characteristics in dogs. The study included 12 normospermic German shepherd dogs (weighed 35 ± 0.5 kg and aged 4 ± 0.5 years). Males received a single melatonin administration (melatonin dimethyl sulfoxide + corn oil via subcutaneous route; MEL; n = 6), while the rest of the animals served as controls (dimethyl sulfoxide + corn oil; Control; n = 6). Males were subjected to routine examination on days -15, 0, 15, 30, 45, and 60. All examined dogs were subjected to Doppler screening, semen collection, and blood sampling. The MEL group showed a significant (P < 0.05) elevation in semen volume, concentration, percentage of sperm motility, and total sperm × 106 / ejaculate compared to other control males. Doppler indices as resistance (RI) and pulsatility (PI) indices declined (P < 0.05) from D 30 (1.02 ± 0.01) until day 60 (0.87 ± 0.02) of treatment. In MEL males, the peak systolic point of velocity (PSV; cm/sec) of the testicular artery elevated (P < 0.05) on day 60 (20.15 ± 0.99) compared to its value on day 0 (17.39 ± 1.84). On D 60, the levels of testosterone (T), estradiol 17-ß (E2), and nitric oxide (NO) elevated (P < 0.05). A negative correlation was detected between testicular volume, scrotal circumference (SC), T levels, Doppler indices, and velocities. In conclusion, single melatonin administration could improve testicular vascularization via increasing Doppler velocities and intratesticular colored areas. In addition, it could improve semen picture and steroids (T and E2) and nitric oxide.

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“…Given that reproductive science, plays a critical role in human health, it is remarkable that approximately only 10% of mammals have been studied in this field (Wildt et al, 2010). Knowledge gained from such cross-species comparisons highlights the diversity that exists (Nagashima and Songsasen, 2021), and how the lack of species-specific studies in reproductive physiology contributes to reduced fertility rates. In a commercial setting, poor fertility often translates to financial losses for producers and compromised welfare arising from repeated invasive veterinary interventions (Li and Winuthayanon, 2017).…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterisation of oviductal organoids from farm and companion animals

Lawson

Ghosh

Blanch

et al. 2022

Preprint

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Organoid technology has provided us with a unique opportunity to study early human development and decipher various steps involved in the pathogenesis of human diseases. The technology is already used in clinics to improve human patient outcomes. However, limited knowledge of the methodologies required to establish organoid culture systems in domestic animals has slowed the advancement and application of organoid technology in veterinary medicine. Here, we have developed a platform to grow organoids from animal tissue samples and characterized oviductal organoids from five domestic animal species. Organoids were grown progressively from single cells derived from the enzymatic digestion of freshly collected equine, bovine, feline, canine, and porcine oviducts. The addition of WNT, TGFB, BMP, Rock, and Notch signalling pathway activators or inhibitors in the culture medium suggested remarkable conservation of the molecular signals involved in oviductal epithelial development and differentiation across species. The gross morphology of organoids from all the domestic species was initially similar. However, some differences in size, complexity, and growth rate were observed and described. Well-defined and synchronised motile ciliated cells were observed in differentiated organoids in mature populations. Histopathologically, oviductal organoids mimicked their respective native tissue. In summary, we have developed a detailed cross-species comparison of oviductal organoid models, which will be valuable for advancing assisted reproductive technologies and fertility studies in these animal species in the future.

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Canid Reproductive Biology: Norm and Unique Aspects in Strategies and Mechanisms

Cited by 19 publications

References 166 publications

Establishment and characterization of oviductal organoids from farm and companion animals

Establishment and characterization of oviductal organoids from farm and companion animals

Single melatonin injection enhances the testicular artery hemodynamic, reproductive hormones, and semen parameters in German shepherd dogs

Establishment and characterisation of oviductal organoids from farm and companion animals

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