Canine Adipose‐Derived Stem Cells: Purinergic Characterization and Neurogenic Potential for Therapeutic Applications

Roszek, Katarzyna; Makowska, Noemi; Czarnecka, Joanna; Porowińska, Dorota; Dąbrowski, Marcin; Danielewska, Justyna; Nowak, Wiesław

doi:10.1002/jcb.25610

Cited by 12 publications

(20 citation statements)

References 41 publications

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“…The stimulatory role of Ado in the proliferation of murine and human MSCs due to the A2A receptors activation is well documented (Katebi et al, ; Riddle et al, ). Consistently, similar results were also obtained for canine MSCs isolated from the adipose tissue (Roszek et al, ). It seems that, independently from the tissue of origin, the undifferentiated MSCs are characterized by high proliferative and regenerative potential additionally increased by Ado supplemented into the extracellular environment.…”

Section: Purinergic Signaling System In Mscssupporting

confidence: 86%

How to influence the mesenchymal stem cells fate? Emerging role of ectoenzymes metabolizing nucleotides

Roszek

Wujak

2018

Journal Cellular Physiology

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Extracellular purines, principally adenosine triphosphate and adenosine, are among the oldest evolutionary and widespread chemical messengers. The integrative view of purinergic signaling as a multistage coordinated cascade involves the participation of nucleotides/nucleosides, their receptors, enzymes metabolizing extracellular nucleosides and nucleotides as well as several membrane transporters taking part in the release and/or uptake of these molecules. In view of the emerging data, it is evident and widely accepted that an extensive network of diverse enzymatic activities exists in the extracellular space. The enzymes regulate the availability of nucleotide and adenosine receptor agonists, and consequently, the course of signaling events. The current data indicate that mesenchymal stem cells (MSCs) and cells induced to differentiate exhibit different sensitivity to purinergic ligands as well as a distinct activity and expression profiles of ectonucleotidases than mature cells. In the proposed review, we postulate for a critical role of these enzymatic players which, by orchestrating a fine-tune regulation of nucleotides concentrations, are integrally involved in modulation and diversification of purinergic signals. This specific hallmark of the MSC purinome should be linked with cell-specific biological potential and capacity for tissue regeneration. We anticipate this publication to be a starting point for scientific discussion and novel approach to the in vitro and in vivo regulation of the MSC properties.

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Section: Purinergic Signaling System In Mscssupporting

confidence: 86%

How to influence the mesenchymal stem cells fate? Emerging role of ectoenzymes metabolizing nucleotides

Roszek

Wujak

2018

Journal Cellular Physiology

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“…Moreover, we postulate that during differentiation, cells become more sensitive to ATP‐mediated signaling, which is often related to cell stress or damage. It was previously observed that MSCs are resistant to the cytotoxic effect induced by ATP [Ferrari et al, ; Roszek et al, ]. Here we present that ATP increases neurogenic transdifferentiation efficiency.…”

Section: Discussionsupporting

confidence: 71%

Neurogenic Differentiation of Mesenchymal Stem Cells Induces Alterations in Extracellular Nucleotides Metabolism

et al. 2016

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The presented results show for the first time that the neurogenic transdifferentiation of hUC-MSCs considerably changes the elements of purinergic signaling profile. Although, it has been demonstrated in the literature that extracellular nucleotides and nucleosides determine the fate of mesenchymal and neural stem cells, there is lack of comprehensive studies on the activity of ecto-enzymes metabolizing nucleotides on the surface of neurogenically induced cells. Our study shows that human UC-MSCs sense the microenvironment and adjust their response to the environmental signals for example, adenine nucleotides and nucleosides. Nucleotides, and not adenosine, signaling alters the biological potential of MSCs-decreases their proliferation rate, increases the neurogenic transdifferentiation efficiency expressed as the number of positively labeled NCAM and A2B5 cells and simultaneously increases the ecto-nucleotidases activity on neural- and glial-committed precursors. Purines implication in the proliferative and neurogenic potential of hUC-MSCs is of strong importance for the in vitro propagation of hUC-MSCs and for their successive therapeutic applications. J. Cell. Biochem. 118: 478-486, 2017. © 2016 Wiley Periodicals, Inc.

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“…GFAP, MAP2, A2B5, S100, TUBB3, nestin, and NEUN are markers of neural cells and can be used as markers of cellular differentiation in vitro. Canine adipose tissue-derived multipotent mesenchymal stromal cells (ASCs) could be induced to express some of these neuronal genes after growing in the presence of the neurogenic inductors valproic acid and forskolin [14,16], basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) [15], in a commercial neurogenic differentiation medium [18] and in a medium containing N2 supplement, brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) [19]. Neurospheres generated from the canine ASCs were used to enrich for neural lineages sufficiently expanded to transplant [19] and, when grown in hypoxia, showed higher expression of the neuronal marker nestin [20].…”

Section: Introductionmentioning

confidence: 99%

Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells

et al. 2020

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Background: The ability of adipose tissue-derived multipotent mesenchymal stromal cells/mesenchymal stem cells (ASCs) to differentiate in neural lineages promises progress in the field of regenerative medicine, especially for replacing neuronal tissue damaged by different neurological disorders. Reprogramming of ASCs can be induced by the growth medium with neurogenic inductors and specific growth factors. We investigated the neural differentiation potential of canine ASCs using several growth media (KEM, NIMa, NIMb, NIMc) containing various combinations of neurogenic inductors: B27 supplement, valproic acid, forskolin, N2-supplement, and retinoic acid. Cells were first preconditioned in the pre-differentiation neural induction medium (mitogenically stimulated; STIM1), followed by the induction of neuronal differentiation. Results: After 3, 6, and 9 days of neural induction, elongated neural-like cells with bipolar elongations were observed, and some oval cells with light nuclei appeared. The expression of neuronal markers tubulin beta III (TUBB3), neurofilament H (NF-H), microtubule-associated protein-2 (MAP2), and glial fibrillary acidic protein (GFAP) was observed using immunocytochemistry, which confirmed the differentiation into neurons and glial cells. Flow cytometry analysis showed high GFAP expression (between 70 and 90% of all cells) after cells had been growing three days in the neural induction medium a (NIMa). Around 25% of all cells also expressed adult neuronal markers NF-H and MAP2. After nine days of ASCs differentiation, the expression of all neural markers was reduced. There were no differences between the neural differentiation of ASCs isolated from female or male dogs. Conclusions: The differentiation repertoire of canine ASCs extends beyond mesodermal lineages. Using a defined neural induction medium, the canine ASCs differentiated into neural lineages and expressed markers of neuronal and glial cells, and also displayed the typical neuronal morphology. Differentiated ASCs can thus be a source of neural cellular lineages for the regenerative therapy of nerve damage and could be useful in the future for therapy or the modelling of neurodegenerative diseases.

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Canine Adipose‐Derived Stem Cells: Purinergic Characterization and Neurogenic Potential for Therapeutic Applications

Cited by 12 publications

References 41 publications

How to influence the mesenchymal stem cells fate? Emerging role of ectoenzymes metabolizing nucleotides

How to influence the mesenchymal stem cells fate? Emerging role of ectoenzymes metabolizing nucleotides

Neurogenic Differentiation of Mesenchymal Stem Cells Induces Alterations in Extracellular Nucleotides Metabolism

Neural differentiation of canine mesenchymal stem cells/multipotent mesenchymal stromal cells

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