Canine Allogeneic Bone Marrow Transplantation

Methotrexate and Citrovorum Factor after Histoincompatible Allogeneic Bone Marrow Transplants in Dogs

“…The survival of the nonengrafted animals in the AMC group is markedly longer than in the nonengrafted animals in the A group (p < 0.005, one-tailed test). No animal given similar TBI alone without a bone marrow transplant lives as long [6]. We actually have no test and no pathognomonic histological finding to distinguish between primary and secondary rejection or early GVHD of the bone marrow in these animals living up to 16 days after transplantation with peripheral blood cell counts below 0.5 X 10® cells/liter.…”

“…However, some comparisons are possible. The incidence of early deaths in the AM group is very high (30%) compared to about 10% of early deaths with 1,000 rad TBI and no posttransplantation immunosuppression [6]. These animals die as a result of septicemia as documented with positive blood cultures and bacterial colonization at post mortem examination.…”

“…Closed-colony, randomly bred, male and female English-American fox hounds were used as donors and beagles as recipients in all experiments. Details of the preparation and selection of the animals, radiation technique, grafting procedure and posttransplantation care have been described earlier [6]. All donor-recipient pairs were mismatched for sex and were histoincompatible at the LD locus as con firmed by mixed leukocyte culture [6].…”

“…Details of the preparation and selection of the animals, radiation technique, grafting procedure and posttransplantation care have been described earlier [6]. All donor-recipient pairs were mismatched for sex and were histoincompatible at the LD locus as con firmed by mixed leukocyte culture [6]. The means of the response indices donor-re cipient and recipient-donor were identical in the A and AMC groups.…”

“…In most animals, bone marrow karyotype analysis was performed to confirm engraftment with previously described techniques [26], Animals showing a progressive decline in the total leuko cyte count after engraftment with a hypocellular post mortem bone marrow were classified as graft rejections. Animals with a cellular bone marrow at post mortem examination and having had clinical signs of GVHD [6] and/or abnormal liver func tion tests were considered to have GVHD.…”