Canine and feline trefoil factor family peptides: Highly conserved molecules with some unique characteristics

Campbell, Bonnie G.; Jabbes, Mohamed

doi:10.1016/j.rvsc.2007.08.008

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…Highest divergence was determined for T368, with the human‐ and canine‐peptides being similar at most. As determined in this work for UTY, substantial homologies and conservation of immune‐reactivity, functionality, proteins, peptides (including MHC‐presentation) and isoforms were already described for canines in comparison to humans, cats, mice, rats, apes and cows by others in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 65%

Minor Histocompatibility Antigen UTY as Target for Graft‐versus‐Leukemia and Graft‐versus‐Haematopoiesis in the Canine Model

et al. 2012

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Male patients with female-stem-cell donors have better prognosis compared to female-to-male combinations due to Y-encoded minor histocompatibility antigens recognized by female-alloimmune-effector lymphocytes in the context of a graft-versus-leukemia (GvL) effect. We provide data in a dog-model that the minor histocompatibility antigen UTY might be a promising target to further improve GvL-immune reactions after allogeneic-stem-cell transplantations. Female-canine-UTY-specific T cells (CTLs) were stimulated in vitro using autologous-DCs loaded with three HLA-A2-restricted-UTY-derived peptides (3-fold-expansion), and specific T cell responses were determined in 3/6 female dogs. CTLs specifically recognized/lysed autologous-female-peptide-loaded DCs, but not na€ ıve-autologous-female DCs and monocytes. They mainly recognized bone-marrow (BM) and to a lower extent DCs, monocytes, PBMCs and B-cells from DLA-identical-male littermates and peptide-loaded T2-cells in an MHC-Irestricted manner. A UTY-/male-specific reactivity was also obtained in vivo after stimulation of a female dog with DLA-identical-male PBMCs. In summary, we demonstrated natural UTY processing and presentation in dogs. We showed that female-dog CTLs were specifically stimulated by HLA-A2-restricted-UTY peptides, thereby enabling recognition of DLA-identical-male cells, mainly BM cells. These observations suggest UTY as a promising candidate-antigen to improve GvL-reactions in the course of immunotherapy.

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Section: Discussionmentioning

confidence: 65%

Minor Histocompatibility Antigen UTY as Target for Graft‐versus‐Leukemia and Graft‐versus‐Haematopoiesis in the Canine Model

et al. 2012

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“…The role of TFF1 or pS2 in gastric carcinogenesis has been investigated in several human and mouse models, and it has been proposed that it might act as a tumor suppressor factor [24,28]. Previously, Campbell and Jabbes [29] have sequenced canine and feline TFFs cDNAs derived from gastric (TFF1, TFF2) and colonic (TFF3) mucosa RNA and showed that the majority of the deduced amino acid sequences of canine and feline TFFs obtained were in agreement with those of other mammalian species (e.g., human, rat, mouse, cow, pig, sheep), supporting the theory that the dog and cat may prove to be useful models for the study of trefoil peptides in various pathologies, such as IBD and gastrointestinal carcinomas. The present study was carried out to investigate whether there is a relationship between TFF1 immunoreactivity and canine gastric carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The genes encoding TFFs were previously characterized in dogs and cats [29]. Schmitz et al [30] assessed TFF gene expression in the gastrointestinal tract from dogs with inflammatory bowel disease (IBD) by PCR and demonstrated that TFF1 expression was significantly upregulated in duodenum of dogs with inflammatory bowel disease (IBD).…”

Section: Introductionmentioning

confidence: 99%

Immunoexpression of Trefoil Factor 1 in Non-Neoplastic and Neoplastic Canine Gastric Tissues

Flores

Castro

Rêma

et al. 2021

Animals

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TFF1 expression is markedly reduced in human GCs, suggesting that TFF1 is a tumor suppressor for human gastric cancer. The present study evaluated the expression and distribution pattern of TFF1 in paraffin-embedded canine gastric tissue samples, including normal mucosa (n = 3), polyps (n = 8), carcinomas (n = 31) and their adjacent non-neoplastic mucosa (n = 30), neoplastic emboli (n = 14), and metastatic lesions (n = 8), by immunohistochemistry (IHC). All normal gastric tissues expressed TFF1 in the superficial foveolar epithelium and mucopeptic cells of the neck region. Most gastric polyps (GPs) displayed immunoreactivity for TFF1 in >75% of the epithelial component. In GCs, the expression of TFF1 was found reduced in 74.2% of the cases. The level of TFF1 expression had a decreased tendency from normal gastric mucosa to GPs and GCs (p < 0.05). No significant differences in the expression of TFF1 were found in GCs, according to age, sex, histological type based on World Health Organization (WHO) and Lauren classification, tumor location, depth of tumor invasion, presence of neoplastic emboli or metastatic lesions. The median survival time of GC patients with preserved and reduced TFF1 immunoexpression were 30 and 12 days, respectively. Kaplan–Meier analysis revealed no significant survival differences between the two groups (p > 0.05). These findings suggest that TFF1 protein may play a role in canine gastric carcinogenesis, and further studies are necessary to define its usefulness as a prognostic indicator in canine gastric carcinoma.

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“…The genes encoding TFFs have been characterized from multiple mammals such as human, mouse, rat, dog, cat, cow, wolf, rhesus monkey, short-tailed opossum, sheep, chimpanzee and pig, as well as frog and toad [5] [9] [13] [14]. Mammalian TFFs are predominantly and profoundly expressed in the gastrointestinal tract, where the expression of each gene and peptide is delicately regulated in a tissue-specific and also topographically complementary manner [15] [16].…”

Section: Introductionmentioning

confidence: 99%

Evolution of Trefoil Factor(s): Genetic and Spatio-Temporal Expression of Trefoil Factor 2 in the Chicken (Gallus Gallus Domesticus)

2011

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Trefoil factors are essential healing initiators participating in mucosal reconstitution and tissue morphogenesis, especially on the surfaces of the gastrointestinal tract. This family has been cloned and characterized predominantly from mammals and amphibians. Avian species ingest stone and grit to help digest food, which may expose their gut to severe physical conditions. To further the understanding of the function of the TFF gene family across species, we undertook this research to clone, sequence, and characterize the spatio-temporal expression patterns of chicken TFF2 (ChTFF2) cDNA. Bioinformatics analysis of the promoter region and deduced amino acid sequence demonstrated that ChTFF2 contained unique characteristics; specifically the chicken promoter has multiple start sites and the protein contains a series of Lys-Lys-Val repeats. Unlike mammals, where TFF2 is detected primarily in the stomach, and occasionally in the proximal duodenum, chicken TFF2 transcripts are found throughout the gastrointestinal tract, with major expression sites in the glandular and muscular stomach as well as evident expression in the colon, small intestine, cecal tonsil and crop. Temporal analysis of intestinal ChTFF2 transcripts by quantitative RT-PCR showed high levels in embryos and a trend of constant expression during embryonic and post-hatch development, with a reduction occurring around hatch. Phylogenetic analysis highlighted the conservation of TFF proteins and functional divergence of trefoil domains, which suggest a transitional role in the bird during evolution.

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Canine and feline trefoil factor family peptides: Highly conserved molecules with some unique characteristics

Cited by 8 publications

References 30 publications

Minor Histocompatibility Antigen UTY as Target for Graft‐versus‐Leukemia and Graft‐versus‐Haematopoiesis in the Canine Model

Minor Histocompatibility Antigen UTY as Target for Graft‐versus‐Leukemia and Graft‐versus‐Haematopoiesis in the Canine Model

Immunoexpression of Trefoil Factor 1 in Non-Neoplastic and Neoplastic Canine Gastric Tissues

Evolution of Trefoil Factor(s): Genetic and Spatio-Temporal Expression of Trefoil Factor 2 in the Chicken (Gallus Gallus Domesticus)

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