Canine antibody response to Blastomyces dermatitidis WI-1 antigen

Klein, Bruce S.; Squires, Richard A.; Lloyd, Janice K. Foyer; Ruge, Daniel R.; Legendre, Alfred M.

doi:10.2460/ajvr.2000.61.554

Cited by 45 publications

(17 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1,3 Agar gel immunodiffusion against the Aantigen of B. dermatitidis is the most commonly used serologic test; however, it does not provide definitive diagnosis and has low sensitivity ranging from 17.4 to 41%. 1,4,5 A recently available EIA test for detection of B. dermatitidis galactomannan antigen in urine and serum has sensitivities of 93.5 and 87.0%, respectively, and antigen concentrations decrease with treatment. 5 Blastomycosis in the 4 dogs reported herein was diagnosed by cytologic examination of the intranasal portion of an intracranial mass (case 2), pulmonary masses (cases 1 and 4), and a skin lesion (case 3).…”

Section: Discussionmentioning

confidence: 99%

Clinical and Magnetic Resonance Imaging Features of Central Nervous System Blastomycosis in 4 Dogs

Lipitz

Rylander

Forrest

et al. 2010

Journal of Veterinary Internal Medicine

View full text Add to dashboard Cite

A 4-year-old female spayed Golden Retriever was evaluated after generalized seizures for 2 days and a 1-month history of decreased appetite, conjunctivitis in the left eye (OS), and pyoderma. Therapy with oral cephalexin (25.6 mg/kg PO q12h), neopolydex ophthalmic solution (OS q6h), and oral phenobarbital (1.6 mg/ kg PO q12h) did not improve clinical signs. Physical examination revealed multifocal nodular skin lesions, granulomatous fundic lesions in both eyes (OU), and uveitis OS. Neurologic examination abnormalities included an absent menace response and direct pupillary light reflex OS, delayed conscious proprioception in all limbs and generalized ataxia. Neuroanatomic localization was forebrain.CBC and serum biochemistry profile results were within reference intervals. On thoracic radiographs, a bilateral interstitial pulmonary pattern was present in the caudal dorsal lung fields with a round, soft tissue opacity (2.5 Â 2 cm) in the left caudal lung.Magnetic resonance imaging (MRI) of the brain was performed with a 1.0 T magnet.a Images included T2-weighted (T2W), fluid-attenuated inversion recovery (FLAIR), and T1-weighted (T1W) images before and after intravenous contrast administration (0.1 mmol/kg gadodiamide).b Multifocal intra-and extra-axial lesions causing a mild left midline shift were identified in the right cerebrum, thalamus, and midbrain). Lesions were also present in the left ocular posterior chamber and temporalis muscles adjacent to affected brain region. Brain lesions were hypointense to grey matter on T1W images and mildly hyperintense with marked perilesional edema on T2W and FLAIR images. After contrast administration, there was enhancement of lesions and meninges (Fig 1). Radiological diagnosis was multifocal brain disease with secondary meningitis and left ocular granuloma or retinal hemorrhage.Cisternal cerebrospinal fluid (CSF) analysis showed 15 white blood cells/mL (reference range, 5), 2 red blood cells/mL, and 25.9 mg/dL protein (reference range, 25). The differential cell count was 30% neutrophils, 60% lymphocytes, and 10% macrophages. CSF immunoflourescence antibody titer (IFAT) for Neospora caninum was 10 (reference, !10 positive).Microscopic examination of an aspirate of the pulmonary mass showed pyogranulomatous inflammation and Blastomyces dermatitidis organisms. Disseminated blastomycosis was presumptively diagnosed and the dog was treated with fluconazole (5 mg/kg PO q12h); phenobarbital (2.5 mg/kg PO q12h), cephalexin (20 mg/ kg PO q12h), and neopolydex (OU q8h) were continued.The dog represented for examination because of acute obtundation 2 weeks later. Inflammation secondary to death of Blastomyces organisms in the central nervous system (CNS) was suspected. After therapy of dexamethasone sodium phosphate (0.07 mg/kg IV once) and prednisone (0.25 mg/kg PO q12h), the dog's clinical signs rapidly improved. Clindamycin (10 mg/kg PO q12h) was added based on a N. caninum serum titer of 400 (reference, !100 indicates exposure).One month later, neurologic examination was norm...

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical and Magnetic Resonance Imaging Features of Central Nervous System Blastomycosis in 4 Dogs

Lipitz

Rylander

Forrest

et al. 2010

Journal of Veterinary Internal Medicine

View full text Add to dashboard Cite

show abstract

“…If the disease is not diagnosed or goes untreated while in the lungs it may become invasive and disseminate to other organs and possibly to the central nervous system where fatal meningitis may develop [2][3][4][5][6]. In dogs a B. dermatitidis infection may disseminate to the eyes, bones, skin or lymph nodes and produce an immune response directed against the yeast phase antigens [7,8]. Blastomycosis, as well as other systemic mycoses, are termed "emerging fungal threats" since they can not only infect individuals with normal immune systems, but also are a cause for concern in hosts with deficiency diseases that compromise the immune system; therefore, a prompt diagnosis and treatment is important to the survival of the patient [2,5,6].…”

Section: Introductionmentioning

confidence: 99%

Antigen Detection in Canine Blastomycosis: Comparison of Different Antibody-Antigen Combinations in Two Competitive ELISAs

Andrae

Birch²,

Bybee³

et al. 2012

OJMM

View full text Add to dashboard Cite

This present study was designed to evaluate four different Blastomyces dermatitidis antibody-antigen combinations (B5896 and T-58 antibodies and B5896 and WI-R antigens) for the detection of antigen in 36 urine specimens from dogs with blastomycosis using a standard indirect ELISA (STD) and a biotin-streptavidin ELISA (B-SA). The antigen detection sensitivity values ranged from 81% (B-SA: T-58 Ab + WI-R Ag) to 100% (STD and B-SA: B5896 Ab + WI-R Ag; B5896 Ab + B5896 Ag) with the antibody-antigen combinations in the two assays. Optimal detection was evidenced when the B5896 Ab was allowed to react with the urine specimens for 30 min at 37˚C and then placed in the B-SA ELISA plates containing the B5896 Ag. The greatest absorbance value obtained with this antibody-antigen combination was 0.903 (range of 0.596 -0.903) as compared to the control value of 1.246. The difference between the control absorbance and the test absorbance values was 0.343 which was considerably greater than the control-test values with the other combinations. This study thus showed that the results obtained in antigen detection assays are dependent upon the antibody used to react with the urine specimens as well as the antigen used in the enzyme immunoassay.

show abstract

“…As with many other diseases, time is of particular importance in terms of diagnosis and treatment [15,16]. Current culture methods may be effective, but very time consuming.…”

Section: Introductionmentioning

confidence: 99%

“…Current culture methods may be effective, but very time consuming. Therefore researchers have focused on the development of immunodiagnostic assays in attempts to provide improved methods for the clinical diagnosis of blastomycosis in humans and animals [2,10,11,[14][15][16][17][18]. Our laboratory has developed various B. dermatitidisyeast phase lysate preparations and utilized these antigens in the ELISA for the detection of antibodies in serum specimens from immunized or infected animals [10,11,13,15].…”

Section: Introductionmentioning

confidence: 99%

Comparison of antibody detection with <i>Blastomycesdermatitidis</i> yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

Christenson¹,

Horton²,

Campbell³

et al. 2011

OJI

View full text Add to dashboard Cite

This present study was designed to evaluate B. dermatitidis antigens, prepared from two isolates (B5896, 597), when the yeast cells were allowed to lyse in distilled water for one day or seven days. The indirect enzyme-linked immunosorbent assay (ELISA) was used to determine the ability of the lysate reagents to detect antibodies in 30 rabbit and 30 dog serum specimens. Mean absorbance values with B5896 lysate antigen ranged from 1.637 (day 1) to 1.461 (day 7) and absorbance values with 597 antigen ranged from 1.579(day 1) to 1.396 (day 7) with the serum specimens from immunized rabbits. Serum specimens from infected dogs yielded absorbance values ranging from 1.672 (day 1) to 1.763 (day 7) with the B5896 and values ranging from 1.909 (day 1) to 1.224 (day 7) with the 597. Optimal reactivity was obtained with the day 1 lysate using both lysate antigens against the rabbit sera and with the 597 antigen against the dog sera. Slightly greater reactivity was evidenced with the day 7 B5896 antigen when the dog sera was tested. Comparative studies are continuing in order to produce an optimal antigenic preparation for antibody detection in blastomycosis.

show abstract

Canine antibody response to Blastomyces dermatitidis WI-1 antigen

Abstract: Anti-WI-1 antibodies are readily detected by RIA in dogs with blastomycosis. Titers become high, decline during treatment, and persist for months. Anti-A antibodies are sometimes detected with AGID, but these decrease quickly.

Cited by 45 publications

References 16 publications

Clinical and Magnetic Resonance Imaging Features of Central Nervous System Blastomycosis in 4 Dogs

Clinical and Magnetic Resonance Imaging Features of Central Nervous System Blastomycosis in 4 Dogs

Antigen Detection in Canine Blastomycosis: Comparison of Different Antibody-Antigen Combinations in Two Competitive ELISAs

Comparison of antibody detection with <i>Blastomycesdermatitidis</i> yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

Contact Info

Product

Resources

About

Canine antibody response to Blastomyces dermatitidis WI-1 antigen

Abstract: Anti-WI-1 antibodies are readily detected by RIA in dogs with blastomycosis. Titers become high, decline during treatment, and persist for months. Anti-A antibodies are sometimes detected with AGID, but these decrease quickly.

Cited by 45 publications

References 16 publications

Clinical and Magnetic Resonance Imaging Features of Central Nervous System Blastomycosis in 4 Dogs

Clinical and Magnetic Resonance Imaging Features of Central Nervous System Blastomycosis in 4 Dogs

Antigen Detection in Canine Blastomycosis: Comparison of Different Antibody-Antigen Combinations in Two Competitive ELISAs

Comparison of antibody detection with &lt;i&gt;Blastomycesdermatitidis&lt;/i&gt; yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

Contact Info

Product

Resources

About

Comparison of antibody detection with <i>Blastomycesdermatitidis</i> yeast lysate antigens in serum specimens from immunized rabbits and infected dogs