Canine conceptus-maternal communication during maintenance and termination of pregnancy, including the role of species-specific decidualization

Kowalewski, Mariusz P.; Pereira, Miguel Tavares; Kazemian, Ali

doi:10.1016/j.theriogenology.2020.01.082

Cited by 27 publications

(33 citation statements)

References 99 publications

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“…The decreased signaling of P4 is a key event in the termination of pregnancy. Accordingly, in the dog, either the decreased availability of circulating P4 closer to term, or the functional blockage of the P4 response with antigestagens, results in parturition or abortion [ 13 , 21 , 35 ]. Consequently, due to the importance of decidual cells for the maintenance of pregnancy, understanding the effects of P4/PGR signaling in these cells is crucial to unveiling the mechanisms of canine parturition.…”

Section: Discussionmentioning

confidence: 99%

Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells

Kazemian

Pereira

Hoffmann

et al. 2022

Animals

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Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigate possible PGR-mediated mechanisms in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with functional PGR-blockers, mifepristone and aglepristone. Effects on decidualization markers, epithelial and mesenchymal factors, and markers of cellular viability were assessed. Decidualization increased the expression of PTGES, PGR, IGF1, and PRLR, along with ECM1, COL4 and CX43, but downregulated IGF2. DUS cells retained their mesenchymal character, and the expression of COL4 indicated the mesenchymal-epithelial transformation. Antigestagen treatment decreased the availability of PTGES, PRLR, IGF1 and PGR. Furthermore, antigestagens decreased the mRNA and protein expression of CX43, and transcriptional levels of ECM1 and COL4. Additionally, antigestagens increased levels of activated-CASP3 (a proapoptotic factor), associated with lowered levels of PCNA (a proliferation marker). These data reveal important aspects of the functional involvement of PGR in canine decidual cells, regarding the expression of decidualization markers and acquisition of epithelial-like characteristics. Some of these mechanisms may be crucial for the maintenance and/or termination of canine pregnancy.

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Section: Discussionmentioning

confidence: 99%

Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells

Kazemian

Pereira

Hoffmann

et al. 2022

Animals

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“…The PC group was considered to represent the uterine status before prepartal luteolysis, namely, the status before birth. As reviewed and investigated in detail, significant endocrine changes occur before birth, such as the prepartum luteolysis (Concannon 2011 ; Kowalewski 2012 ; Kowalewski et al 2015 ; Kowalewski et al 2020 ) as well as significant changes in placental gene expression. Although Kowaleweski and coworkers investigated in detail the changes during canine pregnancy, they focused on the comparison of early, mid pregnancy and prepartum luteolysis (Kowalewski et al 2020 ; Nowak et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…As reviewed and investigated in detail, significant endocrine changes occur before birth, such as the prepartum luteolysis (Concannon 2011 ; Kowalewski 2012 ; Kowalewski et al 2015 ; Kowalewski et al 2020 ) as well as significant changes in placental gene expression. Although Kowaleweski and coworkers investigated in detail the changes during canine pregnancy, they focused on the comparison of early, mid pregnancy and prepartum luteolysis (Kowalewski et al 2020 ; Nowak et al 2019 ). To the best of our knowledge, uterine gene expressions have not been compared between tissues obtained from before and after prepartal luteolysis, with the latter being represented by OD in our study.…”

Section: Discussionmentioning

confidence: 99%

Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?

et al. 2021

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The aetiology of primary uterine inertia (PUI), which is the most common cause of canine dystocia, is still not elucidated. Prostaglandins (PGs) play a crucial role in parturition. We hypothesized that the expression of prostaglandin endoperoxidase synthase 2 (PTGS2), PGF2α synthase (PGFS), and corresponding receptor (PTGFR) is altered in PUI. We investigated PTGS2, PGFS, and PTGFR mRNA expression, and PTGS2 and PGFS protein expression in interplacental (IP) and uteroplacental sites (UP) in bitches with PUI, obstructive dystocia (OD), and prepartum (PC). PTGS2, PGFS, and PTGFR mRNA expression did not differ significantly between PUI and OD (IP/UP). PTGFR ratio in UP was higher in PC than in OD (p = 0.014). PTGS2 immunopositivity was noted in foetal trophoblasts, luminal and superficial glandular epithelial cells, smooth muscle cells of both myometrial layers, and weakly and sporadically in deep uterine glands. PGFS was localized in luminal epithelial cells and in the epithelium of superficial uterine glands. PTGS2 and PGFS staining was similar between PUI and OD, while PGFS protein expression differed between OD and PC (p = 0.0215). For PTGS2, the longitudinal myometrial layer of IP stained significantly stronger than the circular layer, independent of groups. These results do not support a role for PTGS2, PGFS, and PTGFR in PUI. Reduced PGFS expression in IP during parturition compared with PC and the overall lack of placental PGFS expression confirm that PGFS is not the main source of prepartal PGF2alpha increase. The difference in PTGS2 expression between IP myometrial layers warrants further investigation into its physiological relevance.

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“…Presence of pregnancy during the pre‐implantation stage was confirmed by embryo flushing. The onset of the parturition cascade in dogs is associated with trophoblast‐derived PGF2α, which induces the cessation of luteal activity (prepartum luteolysis), reflected in a steep decline of circulating P4 levels (reviewed in Kowalewski et al., 2020 ). Thus, to determine prepartum luteolysis, circulating P4 was measured every 6h from day 58 of pregnancy until the detected levels were below 3ng/ml in three consecutive measurements.…”

Section: Methodsmentioning

confidence: 99%

Luteal expression of factors involved in the metabolism and sensitivity to oestrogens in the dog during pregnancy and in non‐pregnant cycle

Pereira

Papa

Reichler

et al. 2021

Reprod Domestic Animals

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Canine conceptus-maternal communication during maintenance and termination of pregnancy, including the role of species-specific decidualization

Cited by 27 publications

References 99 publications

Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells

Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells

Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?

Luteal expression of factors involved in the metabolism and sensitivity to oestrogens in the dog during pregnancy and in non‐pregnant cycle

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