Canine dental pulp and umbilical cord-derived mesenchymal stem cells as alternative sources for cell therapy in dogs

Utumi, Paulo Henrique; Fracaro, Letícia; Senegaglia, Alexandra Cristina; Fragoso, Felipe Yukio Ishikawa; Miyasaki, Dayane Mayumi; Rebelatto, Cármen Lúcia Kuniyoshi; Brofman, Paulo Roberto Slud; Villanova, José Ademar

doi:10.1016/j.rvsc.2021.08.006

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…The authors revealed that the both DLA matched and mismatched allogenic transplantation are safe and effective for pulp regeneration due to the lack of signs for toxicity and the generation of pulp-like tissues 12 weeks post-transplantation (202). The results are supported by another study revealing canine DPSCs do not express HLA-DR (similar to DLA Class II in dogs) by flow cytometry (49). In addition, one study explored the blood type-and breed-associated immune reaction on allogenic transplantation in equines.…”

Section: Oral Hard Tissue Engineering In An Animal Modelmentioning

confidence: 87%

“…Many studies also reported confirmational expression of pluripotency regulators and proliferative marker either as in genes or proteins, e.g., SRY-box transcription factor (TF) 2 (SOX2), REX1 TF (REX1), octamer-binding TF 4 (OCT4), and homeobox TF Nanog (NANOG), and proliferation marker Ki67 antigen (Ki67), respectively (40)(41)(42). In addition, this literature review presented the results of MSC surface markers both from flow cytometry and polymerase chain reaction (PCR) results, including CD146 (MSC multipotency), CD44 (cell-matrix interaction), CD29 (cell adhesion), CD73 (cell migration and anti-inflammatory property) CD90 (cell adhesion, migration, homing, proliferation, apoptosis, and differentiation), CD105 (cell migration, proliferation, and differentiation), and CD45 (hematopoietic stem cell (HSC) marker) (43)(44)(45)(46)(47)(48)(49).…”

Section: Oral Tissue Originsmentioning

confidence: 99%

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Trends of regenerative tissue engineering for oral and maxillofacial reconstruction in veterinary medicine

Purbantoro,

Taephatthanasagon,

Purwaningrum

et al. 2024

Front. Vet. Sci.

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Oral and maxillofacial (OMF) defects are not limited to humans and are often encountered in other species. Reconstructing significant tissue defects requires an excellent strategy for efficient and cost-effective treatment. In this regard, tissue engineering comprising stem cells, scaffolds, and signaling molecules is emerging as an innovative approach to treating OMF defects in veterinary patients. This review presents a comprehensive overview of OMF defects and tissue engineering principles to establish proper treatment and achieve both hard and soft tissue regeneration in veterinary practice. Moreover, bench-to-bedside future opportunities and challenges of tissue engineering usage are also addressed in this literature review.

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Section: Oral Hard Tissue Engineering In An Animal Modelmentioning

confidence: 87%

Section: Oral Tissue Originsmentioning

confidence: 99%

Trends of regenerative tissue engineering for oral and maxillofacial reconstruction in veterinary medicine

Purbantoro,

Taephatthanasagon,

Purwaningrum

et al. 2024

Front. Vet. Sci.

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“…Scanning Electron Microscopy (SEM) was performed to analyze the morphology of DPSCs using the fixation, dehydration, and metallization protocol described earlier (2.4.2). Osteogenic differentiation was quantified following the protocol described in Utumi et al 2021 [ 33 ]. The samples were washed with PBS (Gibco TM , Grand Island, NY, USA) and distilled water and fixed with 100% ethanol (Merck, Germany) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Biocompatibility of ABS and PLA Polymers with Dental Pulp Stem Cells Enhance Their Potential Biomedical Applications

Barchiki,

Fracaro,

Dominguez

et al. 2023

Polymers

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Polylactic Acid (PLA) and Acrylonitrile–Butadiene–Styrene (ABS) are commonly used polymers in 3D printing for biomedical applications. Dental Pulp Stem Cells (DPSCs) are an accessible and proliferative source of stem cells with significant differentiation potential. Limited knowledge exists regarding the biocompatibility and genetic safety of ABS and PLA when in contact with DPSCs. This study aimed to investigate the impact of PLA and ABS on the adhesion, proliferation, osteogenic differentiation, genetic stability, proteomics, and immunophenotypic profile of DPSCs. A total of three groups, 1- DPSC-control, 2- DPSC+ABS, and 3- DPSC+PLA, were used in in vitro experiments to evaluate cell morphology, proliferation, differentiation capabilities, genetic stability, proteomics (secretome), and immunophenotypic profiles regarding the interaction between DPSCs and polymers. Both ABS and PLA supported the adhesion and proliferation of DPSCs without exhibiting significant cytotoxic effects and maintaining the capacity for osteogenic differentiation. Genetic stability, proteomics, and immunophenotypic profiles were unaltered in DPSCs post-contact with these polymers, highlighting their biosafety. Our findings suggest that ABS and PLA are biocompatible with DPSCs and demonstrate potential in dental or orthopedic applications; the choice of the polymer will depend on the properties required in treatment. These promising results stimulate further studies to explore the potential therapeutic applications in vivo using prototyped polymers in personalized medicine.

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“…For the quantification of adipogenic and osteogenic differentiation, the absorbance was measured by spectrophotometry [ 81 ]. Cells induced to undergo adipogenic differentiation and control cells were washed three times with PBS and fixed with 4% PFA (Sigma-Aldrich, St. Louis, MO, USA) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Quality Control Optimization for Minimizing Security Risks Associated with Mesenchymal Stromal Cell-Based Product Development

Rebelatto,

Boldrini-Leite,

Daga

et al. 2023

IJMS

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Mesenchymal stromal cells (MSCs) have been considered a therapeutic strategy in regenerative medicine because of their regenerative and immunomodulatory properties. The translation of MSC-based products has some challenges, such as regulatory and scientific issues. Quality control should be standardized and optimized to guarantee the reproducibility, safety, and efficacy of MSC-based products to be administered to patients. The aim of this study was to develop MSC-based products for use in clinical practice. Quality control assays include cell characterization, cell viability, immunogenicity, and cell differentiation; safety tests such as procoagulant tissue factor (TF), microbiological, mycoplasma, endotoxin, genomic stability, and tumorigenicity tests; and potency tests. The results confirm that the cells express MSC markers; an average cell viability of 96.9%; a low expression of HLA-DR and costimulatory molecules; differentiation potential; a high expression of TF/CD142; an absence of pathogenic microorganisms; negative endotoxins; an absence of chromosomal abnormalities; an absence of genotoxicity and tumorigenicity; and T-lymphocyte proliferation inhibition potential. This study shows the relevance of standardizing the manufacturing process and quality controls to reduce variability due to the heterogeneity between donors. The results might also be useful for the implementation and optimization of new analytical techniques and automated methods to improve safety, which are the major concerns related to MSC-based therapy.

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Canine dental pulp and umbilical cord-derived mesenchymal stem cells as alternative sources for cell therapy in dogs

Cited by 8 publications

References 29 publications

Trends of regenerative tissue engineering for oral and maxillofacial reconstruction in veterinary medicine

Trends of regenerative tissue engineering for oral and maxillofacial reconstruction in veterinary medicine

Biocompatibility of ABS and PLA Polymers with Dental Pulp Stem Cells Enhance Their Potential Biomedical Applications

Quality Control Optimization for Minimizing Security Risks Associated with Mesenchymal Stromal Cell-Based Product Development

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