Canine distemper virus in primary and continuous cell lines of human and monkey origin

Bussell, Robert H.; Karzon, David T.

doi:10.1007/bf01267905

Cited by 31 publications

(9 citation statements)

References 20 publications

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“…Cytoplasmic inclusions similar to those noted in the present study have been observed in polykaryocytes produced by infection of cell cultures with several different syncytium-forming viruses (3,4,15,17,21,23,28,30), and evidence has been presented which suggests that polykaryocytes contain little or no infective virus (5,34), possibly because of alterations in the cell membrane or other cellular constituents which accompany the fusion process. Cytoplasmic inclusions occurring in polykaryocytes produced by the simian myxovirus SV5 (6) and in cells infected with the syncytium-forming type 2 parainfluenza virus (18,20) or mumps virus (10) have been shown to be composed of fibrils of viral ribonucleoprotein.…”

Section: Discussionsupporting

confidence: 84%

Immunofluorescence and Cytochemical Studies of Visna Virus in Cell Culture

Harter

Hsu

Rose

1967

J Virol

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Sequential morphological changes occurring in sheep choroid plexus cells infected with visna virus were studied by direct immunofluorescence, acridine orange, and hematoxylin and eosin staining methods. Specific immunofluorescence was first detected in the perinuclear cytoplasm of solitary cells 24 hr after infection. As the infection progressed, viral antigen appeared in an increasing number of cells, and rounded globular cells with long slender processes harboring intense fluorescence were seen. Nuclear fluorescence was not observed in infected monolayers. Polykaryocytes formed within 6 hr after inoculation due to the direct cell-fusing effect of the virus inoculum did not show specific fluorescence. Viral antigen was found, however, in the cytoplasm of multinucleated giant cells in cover slips harvested after new infective virus had been released, and later in the course of infection circular fluorescent inclusions were seen in the cytoplasm of polykaryocytes. Comparable eosinophilic inclusions were observed in hematoxylin and eosin preparations, and acridine orange staining of infected monolayers demonstrated similar inclusions which fluoresced with the color characteristic of single-stranded nucleic acid and were susceptible to digestion with ribonuclease. Visna virus appears to be a ribonucleic acid virus which replicates in the cytoplasm.

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Section: Discussionsupporting

confidence: 84%

Immunofluorescence and Cytochemical Studies of Visna Virus in Cell Culture

Harter

Hsu

Rose

1967

J Virol

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“…This conclusion is based upon failure of MAb against hsp 73 to suppress NC polymerase activity and the fact that purified hsp73 does not exhibit the dosage-dependent stimulation/suppression of polymerase observed with hsp72/73. The species-origin of the hsp73 is relevant to CDV transcription since cell lines derived from cattle are permissive to infection by both attenuated and virulent CDV (Bussell & Karzon, 1965;Metzler et al, 1980, I981). Furthermore, bovine and primate hsp73 genes are homologous, exhibiting 70% identity at the nucleotide level (DeLuce-Flaherty & McKay, 1991).…”

Section: Discussionmentioning

confidence: 99%

The Highly Inducible Member of the 70 kDa Family of Heat Shock Proteins Increases Canine Distemper Virus Polymerase Activity

Oglesbee

Liu

Kenney

et al. 1996

Journal of General Virology

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“…The recent field isolated strain of KDK-1 [14] provided by Dr. Mochizuki (Kyoritsu Pharmaceutical Company Ltd, Tokyo, Japan) was isolated from a diseased dog in B95a cells in Japan in 1991 and passaged three times in Vero cells. The attenuated strain of Onderstepoort [2][3][4] provided by Dr. Summers (Cornell University, U.S.A.) was isolated from a North American ranched fox diseased under outbreak of canine distemper in 1930s, and passaged serially in ferrets 57 times, in chicken embryos 208 times, chicken embryo cell culture 62-66 times, ferret kidney cells 13-14 times and in Vero cells more than 100 times. These viruses were passaged once in Vero-DST cells for the stock preparation and stored at -70°C until used.…”

Section: Methodsmentioning

confidence: 99%

The Growth Profiles of Three Types of Canine Distemper Virus on Vero Cells Expressing Canine Signaling Lymphocyte Activation Molecule

Lan

Yamaguchi

Kai

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. To know growth profiles of canine distemper virus (CDV) on Vero cells stably expressing canine signaling lymphocyte activation molecule (Vero-DogSLAMtag; Vero-DST cells), the propagation of three strains of CDV was tested in Vero-DST cells in comparison with parental Vero cells. Strain MD77 could grow well in both cell lines, but demonstrated no syncytium formation or indistinguishable rounding cytopathic effects (CPE) in Vero cells. Strains Onderstepoort and KDK-1 also grew well in Vero-DST cells with apparent syncytium CPE, while they grew less or no efficiently, respectively, in Vero cells. All three CDV strains demonstrated the peak titers, in Vero-DST cells before reaching to an extensive CPE and drastic decrease of titers at/after full CPE. Immunohistochemistry revealed that viral antigens of all CDV strains were found exclusively in the syncytia in Vero-DST cells, while in Vero cells, viral antigen was identified in their single cells for strain MD77 but none for other strains. Thus, every strain of CDV could grow well in Vero-DST cells and behaved differently against Vero cells. These results would be of practical value for workers of CDV because 1) In Vero-DST cells, by observation of distinct syncytium CPE, the highest titer or the best growth of virus could be identified; 2) In Vero cells, various CDV strains could be readily classified after propagation in Vero-DST cells.

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Canine distemper virus in primary and continuous cell lines of human and monkey origin

Cited by 31 publications

References 20 publications

Immunofluorescence and Cytochemical Studies of Visna Virus in Cell Culture

Immunofluorescence and Cytochemical Studies of Visna Virus in Cell Culture

The Highly Inducible Member of the 70 kDa Family of Heat Shock Proteins Increases Canine Distemper Virus Polymerase Activity

The Growth Profiles of Three Types of Canine Distemper Virus on Vero Cells Expressing Canine Signaling Lymphocyte Activation Molecule

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