Canine Epidermal Keratinocytes (CPEK) Grown in Monolayer Are Not Representative of Normal Canine Keratinocytes for Permeability Studies: Pilot Studies

Marsella, Rosanna; Wilkes, Rachel; Ahrens, Kim

doi:10.3390/vetsci9010025

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…These results suggest that TSLP might be involved in the induction of flares in cAD, although CPEK might not be an adequate model for cAD skin. 25 Furthermore, the skin of a HDM-induced cAD model showed a distinctive distribution pattern of TSLP (i.e. a condensed staining pattern at the stratum basale) compared to normal control skin.…”

Section: Interleukin-33mentioning

confidence: 88%

“…PAR‐2 and TLR1/2 are receptors for house dust mite (HDM)‐derived proteases and for membrane proteins of Staphylococcus spp., respectively, and activation may follow exposure. These results suggest that TSLP might be involved in the induction of flares in cAD, although CPEK might not be an adequate model for cAD skin 25 . Furthermore, the skin of a HDM‐induced cAD model showed a distinctive distribution pattern of TSLP (i.e.…”

Section: Updates On Cytokines and Chemokines In Canine Atopic Dermatitismentioning

confidence: 96%

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Update on the role of cytokines and chemokines in canine atopic dermatitis

Tamamoto-Mochizuki

Santoro

Saridomichelakis

et al. 2023

Veterinary Dermatology

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BackgroundCytokines and chemokines play central roles in the pathogenesis of canine atopic dermatitis (cAD). Numerous studies have been published and provide new insights into their roles in cAD.ObjectivesTo summarise the research updates on the role of cytokines and chemokines in the pathogenesis of cAD since the last review by the International Committee on Allergic Diseases of Animals in 2015.Material and MethodsOnline citation databases, abstracts and proceedings from international meetings on cytokines and chemokines relevant to cAD that had been published between 2015 and 2022 were reviewed.ResultsAdvances in technologies have allowed the simultaneous analysis of a broader range of cytokines and chemokines, which revealed an upregulation of a multipolar immunological axis (Th1, Th2, Th17 and Th22) in cAD. Most studies focused on specific cytokines, which were proposed as potential novel biomarkers and/or therapeutic targets for cAD, such as interleukin‐31. Most other cytokines and chemokines had inconsistent results, perhaps as a consequence of their varied involvement in the pathogenesis of different endotypes of cAD.Conclusions and Clinical RelevanceInconsistent results for many cytokines and chemokines illustrate the difficulty of studying the complex cytokine and chemokine networks in cAD, and highlight the need for more comprehensive and structured studies in the future.

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Section: Interleukin-33mentioning

confidence: 88%

Section: Updates On Cytokines and Chemokines In Canine Atopic Dermatitismentioning

confidence: 96%

Update on the role of cytokines and chemokines in canine atopic dermatitis

Tamamoto-Mochizuki

Santoro

Saridomichelakis

et al. 2023

Veterinary Dermatology

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“…Also, to the best of the authors’ knowledge, this is the first report in veterinary medicine on the application of TEER in canine atopic dermatitis. Another study on TEER was done by our group comparing normal keratinocytes and CPEKs [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Differences in Behavior between Normal and Atopic Keratinocytes in Culture: Pilot Studies

Marsella

Ahrens

Wilkes

2022

Veterinary Sciences

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Skin barrier dysfunction is important in atopic dermatitis and can be secondary to inflammation. Observation of keratinocytes in culture may show intrinsic differences. TransEpithelial Electrical Resistance (TEER) measures epithelial permeability. We cultured normal and atopic keratinocytes and found that TEER of atopic keratinocytes was significantly lower (p < 0.0001) than that of normals. Atopic keratinocytes grew upwards, first creating isolated dome-like structures and later horizontally into a monolayer. At time of confluence (D0), atopic keratinocytes were more differentiated, with higher filaggrin gene expression than normals. No differences existed between groups for TJ proteins (claudin, occludin, and Zonula Occludens-1) on D0 and D6. On D6, claudin and occludin were higher than D0, in normal (p = 0.0296 and p = 0.0011) and atopic keratinocytes (p = 0.0348 and 0.0491). Immunofluorescent staining showed nuclear location of filaggrin on D0 and cytoplasmic on D6. ANOVA showed increased cell size from D0 to D6 in both groups (effect of time, p = 0.0076) but no differences between groups. Significant subject effect (p = 0.0022) was found, indicating that cell size was subject-dependent but not disease-dependent. No difference for continuity for TJ protein existed between groups. These observations suggest that decreased TEER in atopics is not linked to TJ differences but is possibly linked to different growth behavior.

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“…Although CPEKs have been largely used in veterinary dermatological research as a more convenient alternative to skin biopsies and primary keratinocytes, concerns have been raised about using CPEKs in vitro as a representative model of canine atopic skin. Recently, Marsella et al (2022) compared skin barrier integrity and permeability using CPEKs and healthy canine keratinocytes. In this study, the authors used transepithelial electric resistance (TEER) and immunofluorescence as methods to assess epithelial permeability.…”

Section: Canine Progenitor Epidermal Keratinocytesmentioning

confidence: 99%

“…Recently, Marsella et al. (2022) compared skin barrier integrity and permeability using CPEKs and healthy canine keratinocytes. In this study, the authors used transepithelial electric resistance (TEER) and immunofluorescence as methods to assess epithelial permeability.…”

Section: In Vitro Inflammatory Skin Disease Modelsmentioning

confidence: 99%

Canine Models of Inflammatory Skin Diseases and Their Application in Pharmacological Research

Gil,

Santoro

2023

Current Protocols

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The purpose of this article is to provide an overview of existing pharmacological models of canine dermatitis. Canine models of dermatitis have contributed significantly to our current understanding of the pathology of dermatitis and to the development of corresponding pharmacological interventions. Specifically, canine atopic dermatitis (AD) is reviewed here, as it is one of the most common inflammatory skin diseases in dogs. Canine AD also shares clinicopathological features with human AD, making the dog a natural and optimal model for human disease. Thus, pharmacological models of canine AD may be uniquely applicable to human pharmacological research. In this article, particular attention is dedicated to relevant in vivo, in vitro, and ex vivo models of canine AD, skin barrier defect models, pruritus models, and skin immunology models. Additionally, models of superficial pyoderma and food allergy are also discussed. With understanding of findings from canine models, researchers can select the most salient features for future pharmacological drug development. © 2023 Wiley Periodicals LLC.

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Canine Epidermal Keratinocytes (CPEK) Grown in Monolayer Are Not Representative of Normal Canine Keratinocytes for Permeability Studies: Pilot Studies

Cited by 3 publications

References 26 publications

Update on the role of cytokines and chemokines in canine atopic dermatitis

Update on the role of cytokines and chemokines in canine atopic dermatitis

Differences in Behavior between Normal and Atopic Keratinocytes in Culture: Pilot Studies

Canine Models of Inflammatory Skin Diseases and Their Application in Pharmacological Research

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