Canine heat shock protein 27 promotes proliferation, migration, and doxorubicin resistance in the canine cell line DTK-F

Lin, An-Ci; Liao, Chien Chang; Huang, Chien-Yi; Liou, Chian-Jiun; Lai, Yu‐Sheng

doi:10.1016/j.tvjl.2015.02.023

Cited by 4 publications

(2 citation statements)

References 66 publications

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“…Over-expression of Hsp27 has also been associated with poor prognosis of various types of cancers in human beings including breast and prostate (Vahid et al 2016;Kang et al 2008;Ciocca and Calderwood 2005). Hsp27 over-expression has also been reported to promote proliferation, migration, and increased doxorubicin resistance in a CMT cell line, DTK-F (Lin et al 2015), suggesting that Hsp27 induces doxorubicin resistance in CMT. A high correlation between Hsp27 expression and tumor invasiveness in association with reduced overall survival has also been reported in malignant canine mammary neoplasia (Romanucci et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

et al. 2019

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In the current study, we have synthesized canine recombinant Hsp27 in E. coli and raised hyperimmune serum against the protein in mice. Using the hyperimmune serum, an indirect ELISA was developed to estimate circulating levels of Hsp27 in dogs with various types of mammary neoplasia and to compare their levels with those of tumor-free apparently healthy dogs. The developed assay had a high specificity (83.3%) and sensitivity (96.6%) for a cut-off value of 0.45 with respect to histopathological examination in discriminating healthy dogs from those bearing mammary tumors. Serum Hsp27 levels were found to be significantly elevated in tumor subjects (20.33 ± 1.23 ng/ml) as compared to healthy controls (6.56 ± 0.51 ng/ml) and the increase in the Hsp27 levels was irrespective of mammary tumor histotypes. However, dogs with grade-II tumors had higher Hsp27 levels as compared to grade-I types. Therefore, Hsp27 can be exploited as one of the 'neoplastic signatures' of canine mammary tumors.

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Section: Discussionmentioning

confidence: 99%

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

et al. 2019

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“…Besides UCPs, also amount and efficiency of mitochondria to produce heat affects body temperature. PGC-1α plays a key role in mitochondrial biogenesis whereas cytochrome c (cyt c) is a crucial component of the electron transport chain in mitochondria and hence these proteins are essential for muscle oxidative capacity (Huttemann et al, 2011 ; Lin et al, 2015 ). Endurance exercise is known to increase PGC-1α levels and mitochondrial biogenesis in muscle, although UCP levels are found to be reduced (Nozu et al, 1992 ; Freyssenet et al, 1996 ; Pilegaard et al, 2000 ; Jones et al, 2003 ; Russell et al, 2003 ; Holloszy, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Voluntary Running Aids to Maintain High Body Temperature in Rats Bred for High Aerobic Capacity

Karvinen

Silvennoinen

et al. 2016

Front. Physiol.

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The production of heat, i.e., thermogenesis, is a significant component of the metabolic rate, which in turn affects weight gain and health. Thermogenesis is linked to physical activity (PA) level. However, it is not known whether intrinsic exercise capacity, aging, and long-term voluntary running affect core body temperature. Here we use rat models selectively bred to differ in maximal treadmill endurance running capacity (Low capacity runners, LCR and High capacity Runners, HCR), that as adults are divergent for aerobic exercise capacity, aging, and metabolic disease risk to study the connection between PA and body temperature. Ten high capacity runner (HCR) and ten low capacity runner (LCR) female rats were studied between 9 and 21 months of age. Rectal body temperature of HCR and LCR rats was measured before and after 1-year voluntary running/control intervention to explore the effects of aging and PA. Also, we determined whether injected glucose and spontaneous activity affect the body temperature differently between LCR and HCR rats at 9 vs. 21 months of age. HCRs had on average 1.3 • C higher body temperature than LCRs (p < 0.001). Aging decreased the body temperature level of HCRs to similar levels with LCRs. The opportunity to run voluntarily had a significant impact on the body temperature of HCRs (p < 0.001) allowing them to maintain body temperature at a similar level as when at younger age. Compared to LCRs, HCRs were spontaneously more active, had higher relative gastrocnemius muscle mass and higher UCP2, PGC-1α, cyt c, and OXPHOS levels in the skeletal muscle (p < 0.050). These results suggest that higher PA level together with greater relative muscle mass and higher mitochondrial content/function contribute to the accumulation of heat in the HCRs. Interestingly, neither aging nor voluntary training had a significant impact on core body temperature of LCRs. However, glucose injection resulted in a lowering of the body temperature of LCRs (p < 0.050), but not that of HCRs. In conclusion, rats born with high intrinsic capacity for aerobic exercise and better health have higher body temperature compared to rats born with low exercise capacity and disease risk. Voluntary running allowed HCRs to maintain high body temperature during aging, which suggests that high PA level was crucial in maintaining the high body temperature of HCRs.

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Heat Shock Protein Expression and Implications in Spontaneous Animal Tumors: Veterinary and Comparative Aspects

Romanucci

Salda

2017

Heat Shock Proteins

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Canine heat shock protein 27 promotes proliferation, migration, and doxorubicin resistance in the canine cell line DTK-F

Cited by 4 publications

References 66 publications

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

Circulating level of heat shock protein 27 is elevated in dogs with mammary tumors

Voluntary Running Aids to Maintain High Body Temperature in Rats Bred for High Aerobic Capacity

Heat Shock Protein Expression and Implications in Spontaneous Animal Tumors: Veterinary and Comparative Aspects

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