Canine leishmaniosis. Modulation of macrophage/lymphocyte interactions by L. infantum

Díaz, Sofía González; Fonseca, Isabel Pereira da; Rodrigues, A.; Martins, Catarina; Cartaxeiro, Clara; Silva, Maria Jesus; Brito, Teresa; Alexandre‐Pires, Graça; Santos‐Gomes, Gabriela

doi:10.1016/j.vetpar.2012.05.004

Cited by 10 publications

(15 citation statements)

References 32 publications

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“…Recent researches describe monocyte differentiation into macrophages phenotypes, as well as macrophages activation and polarization under stimulus of different immunomodulators (Gordon and Martinez, 2010;Diaz et al, 2012;Melo et al, 2014;Murray et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

In vitro infection by Leishmania infantum in the peripheral blood mononuclear cell-derived macrophages from crab-eating foxes ( Cerdocyon thous )

Tuñon

Moura

Jesus

et al. 2015

Veterinary Parasitology

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Section: Discussionmentioning

confidence: 99%

In vitro infection by Leishmania infantum in the peripheral blood mononuclear cell-derived macrophages from crab-eating foxes ( Cerdocyon thous )

Tuñon

Moura

Jesus

et al. 2015

Veterinary Parasitology

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“…The following FITC-conjugated monoclonal antibodies were used: anti-CD3, canine (5 L/mL; Serotec, Kidlington, OX, UK), anti-CD21 canine (15 L/mL; Exbio, Czech Republic) anti-F4/80 mouse (5 L/mL; Abcam, CA, UK) (Diaz et al, 2012) and isotype controls (0.05 mL; BD Biosciences, San Diego, CA, USA). The following monoclonal antibodies conjugated to PE were also used: Anti-human CD279 (PD-1, the PD1 protein shows high degree of homology between the canine and human species (Folkl et al, 2010)), antihuman CD274 (PD-L1), anti-human CD273 (PD-L2) (15 L/mL, BD Biosciences, San Diego, CA, USA) and isotype controls.…”

Section: Antibodiesmentioning

confidence: 99%

“…The costimulatory molecules involved in the response to Leishmania belong to the B7-CD28 family, which transmit activating (CD28) or inhibitory (CTLA-4) signals to T cells (Greenwald et al, 2005). In canine macrophages infected with Leishmania spp., failure occurs in the antigen presentation process, and analysis of the expression of costimulatory molecules indicates decreased expression of B7, which reduces the proliferation of T lymphocytes (Pinelli et al, 1999) and decreases nitric oxide production (Diaz et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

PD-1 function in apoptosis of T lymphocytes in canine visceral leishmaniasis

et al. 2016

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Dogs infected with Leishmania infantum have a reduced number of T lymphocytes. PD-1 (Programmed cell death 1) a new member of the B7-CD28 family that is expressed by immune cells, and its binding to PD-L1 (CD274) or PD-L2 (CD273) induces the deactivation or apoptosis of T cells. This study aimed to evaluate the expression of PD-1 and its ligands, as well as blocking in the induction of apoptosis in T lymphocytes, TNF-α, IL-4 and nitric oxide production by leucokocytes from PBMC and spleen and the parasite load in dogs with visceral leishmaniasis (VL). Our results showed that the expression of PD1 and its ligands was increased in CD3(+) T cells and CD21(+) B lymphocytes within the peripheral blood and splenic mononuclear cells of dogs with VL. In peripheral blood monocytes, only PD-1 ligands exhibited increased expression; however, in spleen macrophages, increased expression of both PD-1 and its ligands was observed. Levels of apoptosis in peripheral blood and splenic T lymphocytes were higher in dogs with VL compared to healthy dogs. Blocking monoclonal antibodies to PD-1 and its ligands in the culture of mononuclear cells from the peripheral blood and spleen decreased the amount of CD3(+) T lymphocyte apoptosis. The concentration of nitric oxide, TNF-α and IL-4 increased in the culture supernatants of peripheral blood mononuclear cells treated with a blocking monoclonal antibody against PD-1. The TNF-α concentration increased in the culture supernatants of splenic cells following all treatments with antibodies blocking PD-1 and its ligands; however, the amount of IL-4 increased only in the presence of a PD-1 blocking agent. Treatment with a PD-1 blocking monoclonal antibody in the mononuclear peripheral blood of dogs with VL reduced the parasite burden while increased TNF-α. We conclude that in canine visceral leishmaniasis, PD-1 and its ligands are involved in the induction of T lymphocyte apoptosis and in regulating the production of nitric oxide, TNF-α, and IL-4, as well as the parasitic load.

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“…A leishmaniose visceral é uma zoonose altamente preocupante em virtude dos sinais clínicos e gravidade variável que pode provocar, além do grande número de indivíduos suscetíveis (Gontijo e Melo, 2004;Diaz et al, 2012). Muitas estratégias têm sido adotadas a fim de se produzir imunobiológicos com capacidade de estimular respostas eficientes e duradouras frente ao parasito causador da doença (Thakur et al, 2015;Darabi et al, 2015;Martins et al, 2016).…”

Section: Introductionunclassified

“…No entanto, as complexas interações estabelecidas entre Leishmania spp. e seus hospedeiros, sobretudo os mecanismos desenvolvidos por esses protozoários para se evadir da resposta imune, representam grandes entraves para a elaboração de vacinas (Diaz et al, 2012;Gradoni et al, 2015;Jain e Jain, 2015).…”

Section: Introductionunclassified

O probiótico Saccharomyces boulardii modula a resposta de IgG2a em camundongos expostos aos antígenos de Leishmania infantum

Lima

Sampaio²,

Silva³

et al. 2019

Revista Brasileira De Ciência Veterinária

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O objetivo do presente estudo foi avaliar os efeitos do probiótico Saccharomyces boulardii na modulação da resposta imune humoral de animais expostos a antígenos de Leishmania infantum. Para isso, 16 camundongos BALB/c foram imunizados com antígeno particulado de Leishmania infantum e divididos em dois grupos experimentais, um composto por animais suplementados e outro por animais não suplementados com o probiótico. Amostras de sangue dos animais foram colhidas semanalmente durante o período experimental e submetidas ao Ensaio da Imunoabsorbância Ligado à Enzima indireto para avaliação dos títulos de IgG totais e o perfil dos isotipos de IgG produzidos (IgG1 e IgG2a). A suplementação com o probiótico não exacerbou a produção de IgG total em comparação ao grupo controle, não havendo diferenças significativas entre os dois grupos. Porém, as soroconversões de IgG2a foram mais elevadas no grupo suplementado, no qual registrou-se um aumento de 1,46 vezes no final do experimento. Assim, a suplementação com S. boulardii foi capaz de modular a resposta de IgG2a/IgG1 nos animais expostos aos antígenos de Leishmania infantum.

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Canine leishmaniosis. Modulation of macrophage/lymphocyte interactions by L. infantum

Cited by 10 publications

References 32 publications

In vitro infection by Leishmania infantum in the peripheral blood mononuclear cell-derived macrophages from crab-eating foxes ( Cerdocyon thous )

In vitro infection by Leishmania infantum in the peripheral blood mononuclear cell-derived macrophages from crab-eating foxes ( Cerdocyon thous )

PD-1 function in apoptosis of T lymphocytes in canine visceral leishmaniasis

O probiótico Saccharomyces boulardii modula a resposta de IgG2a em camundongos expostos aos antígenos de Leishmania infantum

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