Canine leptospirosis: an Overview of the City of Pelotas, Brazil

Félix, Samuel Rodrigues; Felix, Anelize de Oliveira Campello; Colonetti, Karina; Neto, Amilton Clair Pinto Seixas; Tillmann, Mariana Teixeira; Vasconcellos, Flávia Aleixo; Nobre, Márcia de Oliveira; Dellagostin, Odir A.; Silva, Éverton Fagonde da

doi:10.33448/rsd-v9i10.8830

Cited by 2 publications

(3 citation statements)

References 7 publications

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“…It was high in this study (45.3%), promoting contact situations with urine, such as marking territory, using pee pads, sniffing, licking genitals, or locating a humid area within the dwelling. Those practices facilitate the microorganism spreading quickly [28]. Moreover, the probability of infection could be higher, not only for humans but also for other animals in the same household.…”

Section: Discussionmentioning

confidence: 99%

“…We performed serial dilutions with PBS (1:25) to determine antibody titers, beginning with 1:25. A 1:50 dilution was used as a cutoff for seroreactivity [28]. Tests were read after one-hour incubation using a dark field microscope with a 4× objective without a coverslip; agglutination of 50% of the field was used as a positivity indicator in a sample of 20 µL of the antigen-serum mixture.…”

Section: Microagglutination Test (Mat)mentioning

confidence: 99%

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Canine Leptospirosis in a Northwestern Region of Colombia: Serological, Molecular and Epidemiological Factors

2022

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Canine leptospirosis is a zoonosis of epidemiological importance. Dogs are recognized as primary reservoirs of Leptospira interrogans serogroup Canicola and a source of infection to the environment through urine. This study aimed to determine the presence of antibodies against Leptospira in canines from 49 municipalities in the Department of Antioquia, Colombia. We performed a cross-sectional study of dogs included in a neutering control program. We collected 1335 sera samples, assayed by a microagglutination test (MAT), and performed PCR detection in 21 urine samples. We also surveyed 903 dog owners. We found a seroreactivity of 11.2% (150/1335) in Antioquia with titers ≥1:50. Municipalities with the highest number of cases were Belmira (46.1%), Turbo (34.5%), and Concepción (31.0%). L. santarosai was identified by phylogenetic analysis in one urine sample from the municipality of Granada. The most important factor associated with a positive result was the lack of vaccination against leptospirosis (PR 3.3, p < 0.014). Environmental factors such as water presence and bare soil around the household were also associated with Leptospira seroreactivity in the Department of Antioquia. We reviewed a national epidemiological surveillance database for human cases in those municipalities. We found a correlation between the high number of cases in canines and humans, especially in the Uraba. Serological and molecular results showed the circulation of Leptospira. Future public health efforts in the municipalities with the highest numbers of seroreactivity should be directed towards vaccination to prevent animal disease and decrease the probability of transmission of Leptospira. Dogs actively participate in the Leptospira cycle in Antioquia and encourage the implementation of vaccination protocols and coverage.

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Section: Discussionmentioning

confidence: 99%

Section: Microagglutination Test (Mat)mentioning

confidence: 99%

Canine Leptospirosis in a Northwestern Region of Colombia: Serological, Molecular and Epidemiological Factors

2022

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“…We performed serial dilutions with PBS (1:25) to determine antibody titers beginning with 1:25. A 1:50 dilution was used as a cutoff for Seroreactivity 14 . Tests were read after one-hour incubation using a dark field microscope with a 4X objective without a coverslip; agglutination of 50% of the field was used as a positivity indicator in a sample of 20 L of the antigen-serum mixture.…”

Section: Microagglutination Test (Mat)mentioning

confidence: 99%

Canine Leptospirosis in A Northwestern Region of Colombia: Serological, Molecular and Epidemiological Factors

Pérez-García¹,

Monroy²,

Agudelo‐Flórez³

2022

Preprint

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Canine leptospirosis is a zoonosis of epidemiological importance. Dogs are recognized as primary reservoirs of Leptospira interrogans serogroup Canicola and a source of infection to the environment through urine. This study aimed to determine the presence of antibodies against Leptospira in canines from 49 municipalities in the Department of Antioquia, Colombia. We performed a cross-sectional study of dogs included in a neutering control program. We collected 1335 sera samples, assayed by a microagglutination test (MAT), and performed PCR detection in 21 urine samples. We also surveyed 903 dog owners. We found a seroreactivity of 11.2% (150/1335) in Antioquia with titers 1:50. Municipalities with the highest number of cases were Belmira (46.1%), Turbo (34.5%), and Concepción (31.0%). L. santarosai was identified by phylogenetic analysis in one urine sample from the municipality of Granada. The most important factor associated with a positive result was the lack of vaccination against leptospirosis (PR 3.3, p < 0.014). Environmental factors such as water presence and bare soil around the household were also associated with Leptospira seroreactivity in the Department of Antioquia. We reviewed a national epidemiological surveillance database for human cases in those municipalities. We found a correlation between the high number of cases in canines and humans, especially in the Uraba. Serological and molecular results showed the circulation of Leptospira. Future public health efforts in the municipalities with the highest numbers of seroreactivity should be directed towards vaccination to prevent animal disease and decrease the probability of transmission of Leptospira. Dogs actively participate in the Leptospira cycle in Antioquia and encourage the implementation of vaccination protocols and coverage.

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Canine leptospirosis: an Overview of the City of Pelotas, Brazil

Cited by 2 publications

References 7 publications

Canine Leptospirosis in a Northwestern Region of Colombia: Serological, Molecular and Epidemiological Factors

Canine Leptospirosis in a Northwestern Region of Colombia: Serological, Molecular and Epidemiological Factors

Canine Leptospirosis in A Northwestern Region of Colombia: Serological, Molecular and Epidemiological Factors

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