Canine MDCK cell lines are refractory to infection with human and mouse prions

Conservation of a Glycine-rich Region in the Prion Protein Is Required for Uptake of Prion Infectivity

“…Codon 129 homozygotes show increased susceptibility to sporadic Creutzfeldt-Jakob disease (62) and an earlier age of onset for the orally transmitted prion disease kuru (63), and almost all cases of variant Creutzfeldt-Jakob disease are methionine homozygotes (64). Dogs, one of the few mammalian species with discrepancies in the hydrophobic region, have never been reported as being susceptible to prion infection nor can canine-derived Madin-Darby canine kidney cells support prion infection (65). Finally, recent genotyping studies of members of the Fore linguistic region in Papua, New Guinea, have demonstrated a novel polymorphism that provides complete protection against kuru (66).…”

Prion infection of differentiated neurospheres

“…Prion disease is characterized by neuronal loss, astrogliosis, vacuolation and PrP Sc accumulation (Budka, 2003;Jeffrey et al, 2003). Although PrP C expression is an essential condition to enable prion propagation, it alone is not enough to assure replication (Enari et al, 2001;Polymenidou et al, 2008). Differential PrP C expression does not always correlate with prion susceptibility (Vorberg et al, 2004).…”

Cell culture-based influenza vaccines: A necessary and indispensable investment for the future

“…97 Fourth, the use of MDCK cells may be significantly more advantageous for the production of some influenza B virus vaccines. 93 Finally, MDCK cells are refractory to human and mouse prions, 98 and in vitro data suggests that MDCK cell derived components are not allergenic. 99,100 Extensive literature exists on the adaptation of MDCK cells for scaling up and influenza vaccine production.…”