Canine neosporosis: clinical and pathological findings and first isolation of Neospora caninum in Germany

Peters, Martin C.; Wagner, F; Schares, Gereon

doi:10.1007/pl00008499

Cited by 54 publications

(26 citation statements)

References 24 publications

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“…Np21 + /6 + primers were used for lanes 1-7 and Np7/6 + for lanes [8][9][10][11][12][13][14]. A N. caninum-specific DNA fragment was observed in lane 10. organism was not found directly by light microscopy in the CSF [14,22,24]. Neither protozoa in the CSF by light microscopy, nor IFAT antibodies in the CSF, were detected in our study, however, N. caninum-specific DNA was detected using semi-nested PCR.…”

contrasting

confidence: 77%

Detection of <i>Nesopora caninum</i>-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

Ishigaki

Noya

Kagawa³

et al. 2012

The Journal of Veterinary Medical Science

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contrasting

confidence: 77%

Detection of <i>Nesopora caninum</i>-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

Ishigaki

Noya

Kagawa³

et al. 2012

The Journal of Veterinary Medical Science

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“…To isolate protozoan parasites, intermediate host tissues were treated essentially as described by Peters et al (2000). All solutions (buer, medium) used for the isolation were supplemented with penicillin (1,000 units/ml) and streptomycin (1,000 lg/ml) to prevent bacterial growth.…”

Section: Serological Examinations and Pcrmentioning

confidence: 99%

Hammondia heydorni -like oocysts shed by a naturally infected dog and Neospora caninum NC-1 cannot be distinguished

Schares¹,

Heydorn

Cüppers

et al. 2001

Parasitology Research

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This study describes transmission experiments using Hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog. The isolate was designated as H. heydorni-Berlin-1996. Examination of sera from infected intermediate hosts showed immunoblot reactions that resembled patterns observed after Neospora caninum NC-1 infection. Furthermore, N. caninum DNA could be demonstrated in tissue samples (e.g. heart, brain) of experimentally infected intermediate hosts and in oocyst preparations from H. heydorni-Berlin-1996. The isolated oocysts did not induce any detectable disease in any of the inoculated adult intermediate hosts (goats, sheep, gerbils, guinea pigs, multimammate rats, BALB/c mice, SCID mice), even upon immunosuppression. Furthermore, neither histological lesions nor parasite stages could be identified in the tissues of all fetuses recovered from two multimammate rats that had been infected prior to pregnancy. An experiment with one dog fed a second time on infected intermediate host tissue indicated that immunity may prevent repeated oocyst shedding in N. caninum-infected dogs. In addition, the study clearly demonstrates that N. caninum can be readily transmitted by dogs that have ingested exclusively skeletal muscles of infected intermediate hosts. Therefore, the study has consequences for the recommendations for farmers to prevent postnatal transmission of N. caninum to cattle. It indicates that feeding of any tissues of potential intermediate hosts (including sheep, goats, rodents) to final hosts may induce the shedding of oocysts in these hosts and thus pose a risk for post-natal infection of cattle. With respect to oocyst morphology and the infectivity of muscle tissues for final hosts, no differences were seen in comparison with observations made in the past on Isospora bigemina/I. heydorni/H. heydorni. Therefore, earlier studies made on I. bigemina/I. heydorni/H. heydorni have to be re-evaluated critically to determine whether they may have included N. caninum or other protozoan parasites that use dogs as final hosts and have an oocyst morphology resembling that of I. bigemina/I. heydorni/H. heydorni.

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“…For two dogs that were positive for the 152 kDa antigen, only sera were available that had been taken 35 days and later after feeding with infected tissues. Serum collected from a dog congenitally infected with N. caninum (Peters et al 2000) did not recognise the 152 kDa antigen (Fig. 1b, lane 3).…”

Section: Resultsmentioning

confidence: 99%

“…Sera were diluted 1:50 and the conjugate used at a dilution of 1:500. For comparison, a serum from a congenitally N. caninuminfected dog was used (Peters et al 2000).…”

Section: Serological Examinationsmentioning

confidence: 99%

Cyclic transmission of Neospora caninum : serological findings in dogs shedding oocysts

Schares¹,

Heydorn

Cüppers³

et al. 2001

Parasitology Research

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In a previous paper we demonstrated that Hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog could not be distinguished from the isolate Neospora caninum NC-1. The isolate, designated as H. heydorni-Berlin-1996, was cyclically transmitted using dogs as the ®nal hosts. The present study provides information on the antibody responses of the dogs used for the cyclical transmission of this isolate. The majority of dogs that had shed oocysts showed no sero-conversion with respect to N. caninum tachyzoite surface or immunodominant antigens, either in the indirect¯uorescent antibody test or in two Western-blotbased tests. In addition to the examination of responses to immunodominant antigens, we also analysed the antibody reactions of dogs to a high-molecular-weight antigen (152 kDa) in the tachyzoite antigen preparation. The antibodies against this antigen appeared after the dogs had been fed infected intermediate host tissues and shed oocysts. The reaction was observed in dogs between day 35 and day 447 after feeding of intermediate host tissues. Therefore, our study provides initial information on a 152 kDa tachyzoite antigen, which might be a suitable candidate to identify dogs with a history of shedding N. caninum oocysts.

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Canine neosporosis: clinical and pathological findings and first isolation of Neospora caninum in Germany

Cited by 54 publications

References 24 publications

Detection of <i>Nesopora caninum</i>-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

Detection of <i>Nesopora caninum</i>-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

Hammondia heydorni -like oocysts shed by a naturally infected dog and Neospora caninum NC-1 cannot be distinguished

Cyclic transmission of Neospora caninum : serological findings in dogs shedding oocysts

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