Canine Parentage Testing Based on Microsatellite Polymorphisms.

Ichikawa, Yasuaki; Takagi, Kaoru; Tsumagari, Shigehisa; Ishihama, K.; Morita, Mitsuo; Kanemaki, Masato T.; Takeishi, Masatoshi; Takahashi, Hideaki

doi:10.1292/jvms.63.1209

Cited by 36 publications

(30 citation statements)

References 9 publications

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“…To have an accurate parentage test of dogs, the canine applied genetics committee of the international society for animal genetics has demanded EP values higher than 0.9995 (Halverson and Edwards, 2000). The combined EP values of the parentage testing system based on this study was higher than the recommended value, and similar or greater compared to values from other studies: Poongsan, 0.9925 for 11 loci (Cho and Cho, 2003); German Shepherd, 0.9999 for 19 loci (Zajc and Sampson, 1999) and 0.9946 for 10 loci (Koskinen and Bredbacka, 2000); Beagle, 0.9999 for 20 loci (Ichikawa et al, 2001); Greyhound, 0.9993 for 19 loci (Zajc and Sampson, 1999). As the markers selected in this study were highly polymorphic with high PIC values, sufficient values of EP could be obtained even though the number of investigated markers was smaller than that of other studies.…”

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confidence: 69%

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Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

Kang

Kim²,

Min³

et al. 2009

Genes Genet. Syst.

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To investigate the population structure of five dog breeds in South Korea and to validate polymorphic microsatellite markers for the parentage test, microsatellite loci analyses were conducted for two Korean native dog breeds, Poongsan and Jindo, and three imported dog breeds, German Shepherd, Beagle and Greyhound. Overall genetic diversity was high across all dog breeds (expected heterozygosity range: 0.71 to 0.85), although breeds differed in deviations from Hardy-Weinberg equilibrium (HWE). Significant reduction of heterozygosity in the Poongsan and Greyhound breeds was caused by non-random mating and population substructure within these breeds (the Wahlund effects). The close relationship and high degree of genetic diversity for two Korean native dog breeds were substantial. The mean polymorphism information content value was highest in Jindos (0.82) and Poongsans (0.81), followed by Beagles (0.74), Greyhounds (0.72), and German Shepherds (0.66). Accumulated exclusion power values, as an indication of marker validity for parentage tests, were varied but very high across breeds, 0.9999 for Jindos, Poongsans, and Beagles, 0.9997 for Greyhounds, and 0.9995 for German Shepherds. Taken together, the microsatellite loci investigated in this study can serve as suitable markers for the parentage test and as individual identification to establish a reliable pedigree verification system of dog breeds in South Korea. This study also stresses that the population subdivision within breeds can become an important cause of deviation from HWE in dog breeds.

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supporting

confidence: 69%

“…Because German Shepherd, Beagle and Greyhound are popular in South Korea and their genetic diversity has been well studied, they were evaluated in this study for the comparison of results with other studies (Zajc et al, 1997;Zajc and Sampson, 1999;Koskinen and Bredbacka, 2000;Ichikawa et al, 2001;Cho, 2005).…”

mentioning

confidence: 99%

Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

Kang

Kim²,

Min³

et al. 2009

Genes Genet. Syst.

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“…Another bitch was inseminated with only the cryopreserved semen on Days 6, 7 and 8. Paternity for the delivered pups was examined using microsatellite markers, as described previously [8]. …”

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confidence: 99%

Artificial Insemination with Canine Spermatozoa Frozen in a Skim Milk/Glucose-Based Extender

Abe

Lee

Sano

et al. 2008

Journal of Reproduction and Development

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Abstract. Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-thawed in the presence of glycerol was examined. There was a tendency for the proportion of motile spermatozoa exposed to SG-based extender for 3 h to be higher than that exposed for 1 h, but the difference was not significant. The motility and other viability parameters of canine spermatozoa after thawing were similar to those obtained with an egg yolk-based extender. When spermatozoa frozen with SG-based extender containing glycerol after 3 h exposure were transcervically inseminated into 2 recipient bitches, a total of 6 pups were obtained. These results suggest that a simple extender composed of skim milk, glucose and glycerol is useful for cryopreservation of canine spermatozoa, which may contribute to improved exchange of genetic material and efficient production of companion and working dogs, such as guide dogs for the blind. Key words: Canine, Cryopreservation, Skim milk/glucose-based extender, Spermatozoa (J. Reprod. Dev. 54: [290][291][292][293][294] 2008) lthough freezing of canine semen and insemination of canine bitches with frozen-thawed semen is not as commonly used as in bovine and equine animals, successful artificial insemination with frozen canine semen has been well documented [1] since the first conception in 1969 [2]. Cryopreservation of canine spermatozoa offers potential exchange of genetic material, and thus may lead to improvement in the breeding management programs used to produce working dogs. In particular, in guide dog colonies, application of transcervical artificial insemination using frozen canine semen is anticipated to assist with meeting the demand for adequate supply of guide dogs for the blind. Egg yolk is the most commonly used compound in canine semen extenders for protection of spermatozoa from cold shock and disruption during the freezing and thawing process [1]. However, due to a recent outbreak of avian influenza and its triggering of growing concern throughout the world, transportation of frozen or chilled semen exposed to egg yolk has become extremely difficult. Several countries have, in fact, prohibited export and import of canine frozen semen that contains egg yolk. Thus, it is an urgent matter to develop a novel semen extender without egg yolk for use in freezing of canine spermatozoa. As an alterative compound to egg yolk, skim milk seems to be especially suitable as a semen extender in canine species, since a skim milk extender is the most commonly used extender for mouse [3] and goat [4] sperm. We report here successful artificial insemination with canine spermatozoa frozen in a solution containing skim milk, glucose and glycerol. Materials and Methods Colle...

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“…In regard to the polymorphism of Japanese dogs, blood proteins [25] and mitochondrial DNA [19,24,27] have been extensively studied. Recently, several studies utilizing microsatellite markers have been performed [10,14,17,23,26]. In these studies, however, evidence of the Hardy-Weinberg equilibrium (HWE) has not been presented.…”

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confidence: 99%

Microsatellite Polymorphism in Japanese Mongrel Dogs

Oishi

Maeda

Makimura

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. The genetic variability of 182 unrelated mongrel dogs living in various areas of Japan (from Hokkaido to Okinawa) was studied by collecting their blood. Ten microsatellite loci were chosen from different autosomal chromosomes. After combining a few rare adjoining alleles to allelic classes, it was confirmed that the Hardy-Weinberg equilibrium was attained in each locus. The polymorphic information contents (PICs) of the loci, Ren37A11, Ren48E01, AHTk253, ZuBeCa30, Ren277K09, Ren42N13, AHT130, PEZ03, PEZ12, and AHT121, were 0.58, 0.63, 0.67, 0.67, 0.68, 0.71, 0.79, 0.80, 0.80, and 0.80, and the power of discriminations (PDs) were 0. 80, 0.85, 0.87, 0.88, 0.88, 0.89, 0.94, 0.94, 0.94, and 0.94, respectively. The combined mean exclusion chance (MEC) was 0.9995, indicating that these microsatellite loci are useful for kinship testing of Japanese dogs. KEY WORDS: kinship test, microsatellite, polymorphism.

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Canine Parentage Testing Based on Microsatellite Polymorphisms.

Cited by 36 publications

References 9 publications

Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

Artificial Insemination with Canine Spermatozoa Frozen in a Skim Milk/Glucose-Based Extender

Microsatellite Polymorphism in Japanese Mongrel Dogs

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