Canine synovial fluid biomarkers for early detection and monitoring of osteoarthritis

Bakker, Evelien de; Stroobants, Veerle; Vandael, Femke; Ryssen, Bernadette Van; Meyer, Evelyne

doi:10.1136/vr.103982

Cited by 16 publications

(11 citation statements)

References 56 publications

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“…The correlation of arthroscopic, surgical and advanced imaging data with stage-specific changes in tissue samples taken from dogs with specific OA phenotypes would also be possible, for example, correlating analyses of synovial fluid and serum samples with clinical joint scores in dogs with CCL rupture. Similar correlations have previously been performed in experimental models of OA but could also be evaluated in spontaneous dog OA with appropriate staging and radiographic scoring 111,[159][160][161][162] . Dog joints enable longitudinal study using modern imaging techniques (such as CT, MRI and arthroscopy), serial force plate analysis and tissue sampling and have the potential to reveal additional insights into OA at early and late stages compared with mouse models of OA, in which such evaluations are not technically feasible.…”

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confidence: 95%

Spontaneous dog osteoarthritis — a One Medicine vision

et al. 2019

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| Osteoarthritis (OA) is a global disease that, despite extensive research, has limited treatment options. Pet dogs share both an environment and lifestyle attributes with their owners, and a growing awareness is developing in the public and among researchers that One Medicine, the mutual co-study of animals and humans, could be beneficial for both humans and dogs. To that end, this Review highlights research opportunities afforded by studying dogs with spontaneous OA , with a view to sharing this active area of veterinary research with new audiences. Similarities and differences between dog and human OA are examined, and the proposition is made that suitably aligned studies of spontaneous OA in dogs and humans, in particular hip and knee OA , could highlight new avenues of discovery. Developing cross-species collaborations will provide a wealth of research material and knowledge that is relevant to human OA and that cannot currently be obtained from rodent models or experimentally induced dog models of OA. Ultimately , this Review aims to raise awareness of spontaneous dog OA and to stimulate discussion regarding its exploration under the One Medicine initiative to improve the health and well-being of both species.

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confidence: 95%

Spontaneous dog osteoarthritis — a One Medicine vision

et al. 2019

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“…The SF biomarkers can be detected at an early stage of OA, before the presence of radiographic signs ( 23 ). These biomarkers can forecast disease, monitor the response to treatment, and can be used to assess the degree of OA ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Changes in synovial fluid biomarkers after experimental equine osteoarthritis

Wang

et al. 2017

Journal of Veterinary Research

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IntroductionThe study aimed to clarify the changes in the concentration of inflammatory mediators, proteases, and cartilage degradation biomarkers in the synovial fluid of joints in an equine osteoarthritis model.Material and MethodsOsteoarthritis was induced in eight Mongolian horses by a sterile intra-articular injection of amphotericin B, which was injected into the left carpal joint in a dose of 2 mL (25 mg/mL). The control group comprised five horses which were injected with an equal dose of sterile physiological saline into the left carpal joint. Synovial fluid was obtained at baseline and every week after injection. Test methods were based on ELISA.ResultsIn the course of the osteoarthritis, the concentration of biomarkers in joint synovial fluid showed an increasing trend. IL-1, IL-6, MMP-9, MMP-13, ADAMTS-5, CS846, GAG, HA, CTX-II, and COMP concentrations sharply increased before the onset of significant symptoms of lameness, whereas TNF-α, MMP-2, and MMP-3 concentrations rose sharply after the occurrence of such symptoms.ConclusionThe results obtained confirm that the concentrations of IL-1, IL-6, MMP-9, MMP-13, ADAMTS-5, CS846, GAG, HA, CTX-II and COMP increase substantially in equine osteoarthritis, which provides a theoretical basis for the rapid diagnosis of the disease.

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“…Synovial fluid contains an abundance of protein; therefore, α-1 antitripsin (A1T1) was used as the loading control, since it has previously been shown to have consistent levels between Control and OA synovial fluid samples [32]. Matrix metalloproteinase-2 (MMP2) was used as a positive control marker of inflammation, as it has been shown to be elevated in canine synovial fluid samples with OA [33][34][35][36].…”

Section: Biomarker Analysismentioning

confidence: 99%

Evidence of acrolein in synovial fluid of dogs with osteoarthritis as a potential inflammatory biomarker

Herr

Malek

Rochat

et al. 2021

BMC Musculoskelet Disord

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Background Acrolein is a known pro-inflammatory toxic aldehyde, propagating cellular damage and tissue inflammation in humans and animal models of various diseases. Osteoarthritis (OA) has a significant inflammatory component; however, presence of acrolein in synovial fluid of joints with OA has not been previously reported. The first aim of this study was to evaluate evidence of acrolein in the synovial fluid of dogs with OA as well as in Control joints. The second aim was to determine if evidence of acrolein can be detected in synovial fluid samples that have been in a frozen state for long periods of time. Methods In this pilot clinical study, synovial fluid samples were prospectively collected (i.e., New samples) from a single joint of both clinically healthy (New Control, n = 5) and dogs with OA (New OA, n = 16) and frozen until the time of analysis. Additionally, frozen synovial fluid samples from a biobank (i.e., Old samples) were used to evaluate ability to detect evidence of acrolein in long-term stored samples (median of 4.89 years) in Old Control (n = 5) and Old OA (n = 5) samples. Measurements of acrolein in all synovial fluid samples was based on detection of its major protein adduct, N ε - (3-formyl-3, 4-dehydropiperidino)lysine (FDP-lysine), using the western blot method. Synovial fluid matrix metalloproteinase 2 (MMP2) was measured in all samples using the western blot method as a positive control of OA inflammation. Results Acrolein-lysine adduct was detected in both Control (n = 10) and OA (n = 21) groups in both Old and New samples. Acrolein-lysine adduct and MMP2 were detectable at a lower level in the Old compared to New synovial fluid samples; however, the differences were not statistically significant (p > 0.1). The measured MMP2 levels were significantly higher in the OA compared to Control group samples (p = 0.033), but not for acrolein-lysine adduct (p = 0.30). Conclusions This study confirmed evidence of acrolein in canine synovial fluid of both OA and Control groups. Freezing of synovial fluid for up to 5 years does not appear to significantly affect the ability to detect acrolein-lysine adduct and MMP2 in these samples.

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Canine synovial fluid biomarkers for early detection and monitoring of osteoarthritis

Cited by 16 publications

References 56 publications

Spontaneous dog osteoarthritis — a One Medicine vision

Spontaneous dog osteoarthritis — a One Medicine vision

Changes in synovial fluid biomarkers after experimental equine osteoarthritis

Evidence of acrolein in synovial fluid of dogs with osteoarthritis as a potential inflammatory biomarker

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