Cannabidiol Rescues TNF-α-Inhibited Proliferation, Migration, and Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells

Yu, Lina; Zeng, Liting; Zhang, Zeyu; Zhu, Guanxiong; Xu, Zhijie; Xia, Jie; Weng, Jinlong; Pathak, Janak L.

doi:10.3390/biom13010118

Cited by 19 publications

(15 citation statements)

References 57 publications

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“…The time course of ribosome assembly in cells overexpressing DbpA(R331A) showed a slower rate of the overall 50S subunit assembly than that expressing wild-type DbpA (Gentry et al, 2016). Although they could be measured in the 30S peak of the sucrose gradient at 1 mM Mg 2+ , 27S and 35S were not identi ed from either the front or the latter part of the 30S peak through cryoEM analysis in our previous studies, probably due to their low abundance and small size as early pre50S intermediates (Gentry et al, 2016;Yu et al, 2023). Unlike 27S and 35S, both the 45S peak at 1 mM Mg 2+ and pre50S peak at 10 mM Mg 2+ showed clear pre50S particles with varies conformation in this study (Fig.…”

Section: Discussionmentioning

confidence: 79%

“…Our recent studies on the structural distortion of the 30S subunit at different Mg 2+ concentrations also revealed that mature ribosomes exhibit structural instability at low Mg 2+ concentrations, mainly manifested in increased exibility of h17, h44, and rRNA helices and ribosomal proteins in decoding center (Yu et al, 2023). These ndings suggested that special attention must be paid to maintaining Mg 2+ concentration when studying ribosomes, especially ribosome precursors.…”

Section: Discussionmentioning

confidence: 91%

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PTC Remodeling in Pre50S Intermediates: Insights into the Role of DEAD-box RNA Helicase DbpA

Jiang

et al. 2023

Preprint

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Ribosome biogenesis is a complex and fundamental multi-step process in vivo, requiring numerous assembly factors to facilitate and regulate the subunit assembly. The Escherichia coli DEAD-box RNA helicase, DbpA, is known to promote the assembly of the peptidyl transferase center (PTC) during 50S subunit maturation, specifically through the binding of the H92 hairpin in 23S rRNA. In this study, we employed cryoelectron microscope to characterize a series of pre50S snapshots isolated from an E.coli strain overexpressing a DbpA(R331A) mutant in the background of depleting dbpA gene. Our structural analysis defined a collection of late-stage particles along a progressive assembly pathway, revealing intermediates that were immature in the PTC, with domain V undergoing large conformational changes and progressive folding. In the pre50S intermediates, we observed mismatched base-pairings in H73-H74, which define the possible unwinding substrate of DbpA. Furthermore, the structures identified RsfS and YjgA as participating in 50S maturation with DbpA, and label-free quantitive mass spectrum revealed the involvement of multiple assembly factors and rRNA modification enzymes in these intermediates. In summary, our study provides mechanistic insights into how the DbpA, in concert with other assembly factors, facilitates the maturation of the 50S subunit in prokaryotes.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 91%

PTC Remodeling in Pre50S Intermediates: Insights into the Role of DEAD-box RNA Helicase DbpA

Jiang

et al. 2023

Preprint

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“…In 2023, research was carried out to evaluate the influence of CBD on the migration and differentiation of pulp stem cells and it was observed that CBD attenuated the production of proinflammatory cytokines stimulated by TNF-alpha in pulp stem cells; This information corroborates the hypothesis that CBD is a possible therapeutic agent to treat pulpitis and promote dentin/pulp regeneration in regenerative cell therapy techniques within the field of endodontics, although additional investigations are still needed (Yu et al, 2023). There is even a cannabinoid-Contribuciones a Las Ciencias Sociales, São José dos Pinhais, v.17, n.5, p. 01-13, 2024 based medicine in the patent process that aims to treat and prevent dental pulp diseases (Stahl, 2023).…”

Section: Introductionmentioning

confidence: 71%

Cannabinoids as dental pulp capping agents: a scoping review protocol

Taques,

Borges,

Faraht

et al. 2024

CLCS

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Exposure of the dental pulp and its contamination with bacteria from the oral environment can lead to pulp inflammation, toothache, necrosis and, in immature teeth, interruption of the rhizogenesis process; Therefore, it makes sense to suggest that bioactive materials can lead to the maintenance of pulp viability. Cannabinoids have been investigated in different areas of health and have already been studied in relation to their influence on dental pulp cells and apical papilla stem cells. This work proposes a protocol for the development of a scoping review with the objective of mapping the available literature on the use of cannabinoids as pulp protection agents. This scoping review protocol was prepared following the Peters methodology and the recommendations of the reviewer's manual for scoping reviews of the Joanna Briggs Institute (JBI) and PRISMA-ScR, and was registered in the Open Science Framework (OSF) under the DOI: https://doi.org/10.17605/OSF.IO/HYD28. Searches will be carried out in the Pubmed, Web of Science, Scopus, Cochrane, LILACS and VHL Portal databases, as well as in gray literature repositories. Data analysis will be descriptive and will involve both qualitative and quantitative data, from which a narrative synthesis will be constructed on the use of cannabinoids as protective agents for dental pulp. It is expected that the results obtained in this scoping review will provide support for future debates related to the topic and so that knowledge gaps found during the development of the review can be emphasized.

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“…DPSCs can also migrate to the lesion site to differentiate into target cells to repair the injured tissue. However, studies have shown that MSCs may be transformed in response to cytokines in the inflammatory environment, and thus lose their immunomodulatory ability [187]. The effect of an inflammatory microenvironment on MSCs in autoimmune diseases needs further study.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Mechanism and Potential Application of Dental Pulp-Derived Stem Cells in Immune-Mediated Diseases

Tian

Tang

et al. 2023

IJMS

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) derived from dental pulp tissue, which have high self-renewal ability and multi-lineage differentiation potential. With the discovery of the immunoregulatory ability of stem cells, DPSCs have attracted much attention because they have similar or even better immunomodulatory effects than MSCs from other sources. DPSCs and their exosomes can exert an immunomodulatory ability by acting on target immune cells to regulate cytokines. DPSCs can also migrate to the lesion site to differentiate into target cells to repair the injured tissue, and play an important role in tissue regeneration. The aim of this review is to summarize the molecular mechanism and target cells of the immunomodulatory effects of DPSCs, and the latest advances in preclinical research in the treatment of various immune-mediated diseases, providing new reflections for their clinical application. DPSCs may be a promising source of stem cells for the treatment of immune-mediated diseases.

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Cannabidiol Rescues TNF-α-Inhibited Proliferation, Migration, and Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells

Cited by 19 publications

References 57 publications

PTC Remodeling in Pre50S Intermediates: Insights into the Role of DEAD-box RNA Helicase DbpA

PTC Remodeling in Pre50S Intermediates: Insights into the Role of DEAD-box RNA Helicase DbpA

Cannabinoids as dental pulp capping agents: a scoping review protocol

Immunomodulatory Mechanism and Potential Application of Dental Pulp-Derived Stem Cells in Immune-Mediated Diseases

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