Cannabinoid CB2 receptors: Immunohistochemical localization in rat brain

Gong, Jian Ping; Onaivi, Emmanuel S.; Ishiguro, Hiroki; Liu, Qingrong; Tagliaferro, Patricia; Brusco, Alicia; Uhl, George R.

doi:10.1016/j.brainres.2005.11.035

Cited by 720 publications

(602 citation statements)

References 35 publications

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“…Involvement of CB 2 receptors in the effect of WIN55212-2 in these experiments is unlikely, because the density of CB 2 receptors is generally very low in the brain, and CB 2 receptors are mostly localized on microglial cells and astrocytes (Munro et al, 1993;Fernandez-Ruiz et al, 2007). However, it must be noted that neuronal CB 2 receptors at low densities were observed in some brain regions recently (van Sickle et al, 2005;Gong et al, 2006;Brusco et al, 2008;Suarez et al, 2008;Lanciego et al, 2011).…”

Section: Discussionmentioning

confidence: 82%

Exogenous and Endogenous Cannabinoids Suppress Inhibitory Neurotransmission in the Human Neocortex

Kovacs

Knop

Urbanski

et al. 2011

Neuropsychopharmacol

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Activation of CB 1 receptors on axon terminals by exogenous cannabinoids (eg, D 9 -tetrahydrocannabinol) and by endogenous cannabinoids (endocannabinoids) released by postsynaptic neurons leads to presynaptic inhibition of neurotransmission. The aim of this study was to characterize the effect of cannabinoids on GABAergic synaptic transmission in the human neocortex. Brain slices were prepared from neocortical tissues surgically removed to eliminate epileptogenic foci. Spontaneous GABAergic inhibitory postsynaptic currents (sIPSCs) were recorded in putative pyramidal neurons using patch-clamp techniques. To enhance the activity of cannabinoidsensitive presynaptic axons, muscarinic receptors were continuously stimulated by carbachol. The synthetic cannabinoid receptor agonist WIN55212-2 decreased the cumulative amplitude of sIPSCs. The CB 1 antagonist rimonabant prevented this effect, verifying the involvement of CB 1 receptors. WIN55212-2 decreased the frequency of miniature IPSCs (mIPSCs) recorded in the presence of tetrodotoxin, but did not change their amplitude, indicating that the neurotransmission was inhibited presynaptically. Depolarization of postsynaptic pyramidal neurons induced a suppression of sIPSCs. As rimonabant prevented this suppression, it is very likely that it was due to endocannabinods acting on CB 1 receptors. This is the first demonstration that an exogenous cannabinoid inhibits synaptic transmission in the human neocortex and that endocannabinoids released by postsynaptic neurons suppress synaptic transmission in the human brain. Interferences of cannabinoid agonists and antagonists with synaptic transmission in the cortex may explain the cognitive and memory deficits elicited by these drugs.

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Section: Discussionmentioning

confidence: 82%

Exogenous and Endogenous Cannabinoids Suppress Inhibitory Neurotransmission in the Human Neocortex

Kovacs

Knop

Urbanski

et al. 2011

Neuropsychopharmacol

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“…CNR2 has been observed in the brainstem, 21 cerebellum 22 and several other regions of the brain. 23 We also found that expression of Cnr2 is altered in response to cocaine and heroin (manuscript in preparation). In view of our findings that CNR2 is expressed in the mammalian brain, and that this expression is altered in response to addictive drugs, we hypothesized that genetic variants of CNR2 may have a significant effect on alcohol dependency.…”

Section: Introductionmentioning

confidence: 71%

Involvement of cannabinoid CB2 receptor in alcohol preference in mice and alcoholism in humans

et al. 2006

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We tested if cannabinoid type 2 receptor (CB2) in the central nervous system plays a role in alcohol abuse/dependence in animal model and then examined an association between the CB2 gene polymorphism and alcoholism in human. Mice experiencing more alcohol preference by drinking showed reduced Cb2 gene expression, whereas mice with little preference showed no changes of it in ventral midbrain. Alcohol preference in conjunction with chronic mild stress were enhanced in mice treated with CB2 agonist JWH015 when subjected to chronic stress, whereas antagonist AM630 prevented development of alcohol preference. There is an association between the Q63R polymorphism of the CB2 gene and alcoholism in a Japanese population (P ¼ 0.007; odds ratio 1.25, 95% CI, (1.06-1.47)). CB2 under such environment is associated with the physiologic effects of alcohol and CB2 antagonists may have potential as therapies for alcoholism.

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“…The location of CB2 receptor is more restricted, mainly at the peripheral level (the spleen, pharyngeal amygdales, B lymphocytes and to a lesser extent, monocytes and T lymphocytes) [61] . Recently, CB2 expression has been demonstrated in activated microglia and also in neurons, but its level is lower than that of CB1 receptor [62][63][64][65] .…”

Section: Target Receptorsmentioning

confidence: 99%

“…A number of investigations showed that activation of CB2 receptor has anti-inflammatory therapeutic potential in central nervous system diseases, such as multiple sclerosis, traumatic brain injury and Alzheimer's disease [145][146][147] . Recently, it has been shown that CB2 receptor is also detected in resident inflammatory cells within the brain such as microglia [62][63][64][65]148] , and that hypoxia-ischemia induces its expression in the brain [144,149] .…”

Section: Therapeutic Potential Of Cannabinoid After Hypoxia-ischemiamentioning

confidence: 99%

Cannabinoid as a neuroprotective strategy in perinatal hypoxic-ischemic injury

2011

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Perinatal hypoxia-ischemia remains the single most important cause of brain injury in the newborn, leading to death or lifelong sequelae. Because of the fact that there is still no specific treatment for perinatal brain lesions due to the complexity of neonatal hypoxic-ischemic pathophysiology, the search of new neuroprotective therapies is of great interest.In this regard, therapeutic possibilities of the endocannabinoid system have grown lately. The endocannabinoid system modulates a wide range of physiological processes in mammals and has demonstrated neuroprotective effects in different paradigms of acute brain injury, acting as a natural neuroprotectant. Concerning perinatal asphyxia, the neuroprotective role of this endogenous system is emerging these years. The present review mainly focused on the current knowledge of the cannabinoids as a new neuroprotective strategy against perinatal hypoxic-ischemic brain injury.

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Cannabinoid CB2 receptors: Immunohistochemical localization in rat brain

Cited by 720 publications

References 35 publications

Exogenous and Endogenous Cannabinoids Suppress Inhibitory Neurotransmission in the Human Neocortex

Exogenous and Endogenous Cannabinoids Suppress Inhibitory Neurotransmission in the Human Neocortex

Involvement of cannabinoid CB2 receptor in alcohol preference in mice and alcoholism in humans

Cannabinoid as a neuroprotective strategy in perinatal hypoxic-ischemic injury

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